A, B) Resting light intensity (RLI) images through the cranial window and pseudocolored difference maps showing the peak responses evoked by 3 concentrations of MV from representative male ( A), M01) and female ( B), F01) subjects. The circled callouts indicate the example glomeruli in ( D, E). ( C) Mean ± SEM number of odorant-evoked glomerular responses plotted as a function of odorant concentration. The inset shows the main effect of group pooled across concentrations and is scaled to the same y-axis as ( C). ( D, E) Individual male ( D), M01) and female ( E), F01) glomeruli showing sample odorant response selectivity patterns. The pseudocolored difference maps that were evoked by MV, BA, 2HEX, and 2M2B are shown (top) with all 4 corresponding fluorescent records superimposed per glomerulus (bottom). Individual traces represent 4-trial averages per odorant and the boxed portion of the traces indicates the frames that were used to generate the response maps in ( A,B and D,E) and the analyses in ( C and F). ( F) The mean ± SEM number of odorants that evoked a measurable response in each glomerulus are plotted for male and female glomerular populations. Ns indicate the number of glomeruli per group. The y-axis ranges from 1–4 because each individual glomerulus was categorized as responding to 1–4 odorants in the panel. ( G, H) Glomerular response maps that were evoked by 15 au 2HEX in a representative male mouse ( G), M06) and a representative female mouse ( H, F10). ( I) Fluorescent records that correspond to the glomerular callouts in ( G, H). The boxed portion of the fluorescent records indicates the frames that were used to generate the peak response maps in ( G, H) and the analyses that are summarized in ( J). Solid lines ± shaded regions represent the mean ± SEM fluorescent record across 4 repeated trials for each glomerulus. Each trace is scaled relative to its individual maximum. ( J) Mean ± SEM odorant-evoked change in fluorescence (ΔF) plotted as a function of odorant concentration. The inset shows the main effect of group pooled across concentrations and is scaled to the same y-axis as J.