Figure 1 : Odorant-evoked glomerular response maps contain a greater number of glomeruli receiving synaptic input in unmanipulated females than in unmanipulated males.

From: Differences in peripheral sensory input to the olfactory bulb between male and female mice

Figure 1

(A,B) Resting light intensity (RLI) images through the cranial window and pseudocolored difference maps showing the peak responses evoked by 3 concentrations of MV from representative male (A), M01) and female (B), F01) subjects. The circled callouts indicate the example glomeruli in (D,E). (C) Mean ± SEM number of odorant-evoked glomerular responses plotted as a function of odorant concentration. The inset shows the main effect of group pooled across concentrations and is scaled to the same y-axis as (C). (D,E) Individual male (D), M01) and female (E), F01) glomeruli showing sample odorant response selectivity patterns. The pseudocolored difference maps that were evoked by MV, BA, 2HEX, and 2M2B are shown (top) with all 4 corresponding fluorescent records superimposed per glomerulus (bottom). Individual traces represent 4-trial averages per odorant and the boxed portion of the traces indicates the frames that were used to generate the response maps in (A,B and D,E) and the analyses in (C and F). (F) The mean ± SEM number of odorants that evoked a measurable response in each glomerulus are plotted for male and female glomerular populations. Ns indicate the number of glomeruli per group. The y-axis ranges from 1–4 because each individual glomerulus was categorized as responding to 1–4 odorants in the panel. (G,H) Glomerular response maps that were evoked by 15 au 2HEX in a representative male mouse (G), M06) and a representative female mouse (H, F10). (I) Fluorescent records that correspond to the glomerular callouts in (G,H). The boxed portion of the fluorescent records indicates the frames that were used to generate the peak response maps in (G,H) and the analyses that are summarized in (J). Solid lines ± shaded regions represent the mean ± SEM fluorescent record across 4 repeated trials for each glomerulus. Each trace is scaled relative to its individual maximum. (J) Mean ± SEM odorant-evoked change in fluorescence (ΔF) plotted as a function of odorant concentration. The inset shows the main effect of group pooled across concentrations and is scaled to the same y-axis as J.