Figure 1

From: Environmental DNA in subterranean biology: range extension and taxonomic implications for Proteus

Figure 1

Evaluation of Proteus eDNA presence in a sample from the observed outcome of (a) SYBR qPCR assay and (b) TaqMan qPCR assay. Two mitochondrial DNA (mtDNA) regions (“genes”) were searched for in (a) and one mtDNA region was searched for in (b). The first run in both (a) and (b) included two concentrations (“dilutions”) of the template (data were pooled in b). All assays were performed in three parallel reactions (“wells”). See also the Methods section.