Gene flow is an important component in evolutionary biology; however, the role of gene flow in dispersal of herbicide-resistant alleles among weed populations is poorly understood. Field experiments were conducted at the University of Nebraska-Lincoln to quantify pollen-mediated gene flow (PMGF) from glyphosate-resistant (GR) to -susceptible (GS) common waterhemp using a concentric donor-receptor design. More than 130,000 common waterhemp plants were screened and 26,199 plants were confirmed resistant to glyphosate. Frequency of gene flow from all distances, directions, and years was estimated with a double exponential decay model using Generalized Nonlinear Model (package gnm) in R. PMGF declined by 50% at <3 m distance from the pollen source, whereas 90% reduction was found at 88 m (maximum) depending on the direction of the pollen-receptor blocks. Amplification of the target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), was identified as the mechanism of glyphosate resistance in parent biotype. The EPSPS gene amplification was heritable in common waterhemp and can be transferred via PMGF, and also correlated with glyphosate resistance in pseudo-F2 progeny. This is the first report of PMGF in GR common waterhemp and the results are critical in explaining the rapid dispersal of GR common waterhemp in Midwestern United States.
Gene flow refers to both the movement and introduction of genes and gene complexes into ‘allochthonous (distant) gene pools’1. Along with gene mutation and natural selection, gene flow is an evolutionary force affecting plant species2,3. Andrews4 demonstrated that natural selection, genetic drift, and gene flow do not work in isolation, and that the presence of one or more of these factors in a population leads to violation of the Hardy-Weinberg assumptions, causing evolution to take place. Spatial restrictions on gene dispersal lead to non-random mating that can result in the transformation of a population-subdivision into genetic neighborhoods, whereas extensive gene flow at the landscape scale promotes homogeneity among plant populations5,6.
The evolution of herbicide-resistant weeds is a growing threat to modern agriculture and global food security7. Gene mutations, initial frequency of resistant alleles, inheritance of resistance genes, fitness, and gene flow are the key factors contributing to the evolution and spread of herbicide-resistant weeds, with the rate of gene flow higher than the rate of mutation in nature8. Pollen-mediated gene flow (PMGF) has been occurring since the existence of higher plants and has greatly influenced genetic diversity and the adaptation of populations over time9; however, the recent scientific attention to this topic has resulted from concerns regarding transgene movement from genetically modified to non-genetically modified (conventional) crops, as well as their wild relatives10,11,12. Several studies were conducted as part of the risk assessment and environmental biosafety for landscape-level cultivation of herbicide-tolerant crops13,14; however, limited scientific literature is available on PMGF among different populations of weed species. For example, PMGF has been reported in only a few weed species, including barnyardgrass (Echinochloa crus-galli [L.] Beauv.)15, common lambsquarters (Chenopodium album L.)16, kochia (Kochia scoparia [L.] Schrad.)17, Palmer amaranth (Amaranthus palmeri S. Wats.)18, rigid ryegrass (Lolium rigidum Gaud.)19, weedy beet (Beta vulgaris L.)20, and wild oat (Avena fatua L.)21.
Common waterhemp (Amaranthus rudis Sauer), a summer annual broadleaf weed, is the most commonly encountered weed species in the Midwestern United States22. It is a dioecious species belonging to the subgenus Acnida (L.) Aellen ex K. R. Robertson of the genus Amaranthus23. The invasion of common waterhemp in row crop production systems is one of the most drastic events facing agriculture in the Midwest24. From the time of its first report in Oklahoma in 1830, common waterhemp has been continuously moving toward the northern and eastern parts of the United States, transforming from a rare wetland weed to a major problem weed25,26. Over the last several years, common waterhemp has adapted to a wide range of climatic gradients including severe dry conditions27, and can now be found from the arid regions of Texas to the humid/sub-humid regions of Maine28.
Glyphosate-resistant (GR) common waterhemp was first reported in Missouri29, and has now been confirmed in 18 states in the United States30, including Nebraska31. Its dioecy and anemophilous nature are believed to promote the rapid spread of the herbicide-resistant alleles in an agricultural landscape via pollen migration32. Common waterhemp pollen grains are spherical-shaped, polypantoporate (pollen grain with apertures spread over the surface in a regular pattern), and very small in size (18.5 μm diameter), a formation believed to favor their long-distance dispersal33,34. The rough surface of the pollen grain also creates a boundary layer of turbulence on its exterior, reducing the resistance created when the pollen travels through the air35. The lower pollen settling velocity of common waterhemp (0.0185 to 0.021 m s−1) compared to other major wind-pollinated crops (e.g., corn, which has a pollen settling velocity of 0.33 m s−1 and a pollen diameter of 90 to 100 μm) helps pollen grains to travel longer distances under pollen competition36,37.
An overproduction of the enzyme EPSPS (5-enolpyruvylshikimate-3-phosphate synthase, the biological target of herbicide glyphosate) through additional EPSPS gene copies was responsible for glyphosate resistance in common waterhemp biotypes collected from several Midwestern states38,39. In a GR common waterhemp biotype from Kansas, Dillon40 reported that the amplified EPSPS copies were located on a pair of homologous chromosomes, and that the EPSPS copies clustered near the centromeres of those chromosomes. EPSPS gene amplification was also reported in a GR biotype of Palmer amaranth collected from Georgia, but fluorescence in situ hybridization (FISH) analysis suggested that the amplified genes were dispersed throughout the genome41.
Because the majority of herbicide-resistant traits are nuclear-inherited and can spread via PMGF8, herbicide resistance can be used as an excellent marker to estimate PMGF. The objective of this study was to quantify the frequency of PMGF under field conditions in common waterhemp using glyphosate resistance as a selective marker. This study was conducted using a five-step approach: (i) reviewing literature and constructing hypothesis, (ii) conducting field experiments, (iii) screening for resistance (in greenhouse and laboratory), (iv) fitting statistical models, and (v) predicting gene flow frequency. The null hypothesis for this study was “Gene flow will not occur from glyphosate-resistant to -susceptible (GS) common waterhemp,” which was unrealistic. The hypothesis was constructed to increase the likelihood of detection of the minimum frequency of gene flow and to reduce the type II error.
Flowering was initiated on July 1, 2013 and July 12, 2014, with maximum flowering occurred 2 weeks after flower initiation (Table S1). The common waterhemp plants beyond 5 m distance from the pollen-donor block began flowering a few days later than those in the pollen-donor block or in the proximity of the pollen source. It was believed that the common waterhemp plants in both the pollen-donor block and the closer distances were growing in a higher density compared to the plants at the farthest distances, thus causing a brief gap (<5 days) in the initiation of flowering. The total flowering period lasted about 5 and 4 weeks in 2013 and 2014, respectively. Therefore, a season-flowering synchrony of >85% was ensured (Table S2).
Early and late-season temperature was slightly lower than the temperature during flowering period and mean daily temperature ranged from 17.0 to 30.5 °C in July and early-Aug in 2013 and 2014 (Fig. S1). Pearson correlation coefficients (r) showed a significant correlation between frequency of gene flow and wind parameters [wind speed, wind frequency (frequency of time/hours during which the wind blows toward a certain direction), and wind run (calculated by multiplying the average wind speed by the wind frequency for a certain direction42)]; however, the correlation (r ≥ 0.29) was significant up to 15 m (from the pollen source) for wind speed and 25 m for wind run (Table 1). Correlation between wind parameters and frequency of gene flow at the closer distances (within 5 m) was higher (≥0.48) than at the farthest distances. No negative correlation was observed, suggesting that the wind had a great influence on PMGF in common waterhemp.
Wind flow patterns were similar in 2013 and 2014, though the wind speed and wind frequency differed. Wind during both years blew mostly from the S and SE directions (Fig. 1). The average wind speed during the flowering period in 2014 was lower (1.4 m s−1) than the average wind speed in 2013 (2.1 m s−1) (Fig. S2).
Modeling PMGF and Prediction of Distances
Determining the sample size a priori allows a researcher to reduce the sampling cost and time without losing statistical precision to quantify gene flow43. In 2013 and 2014, more than 130,000 common waterhemp seedlings were screened in the greenhouse to detect PMGF and a minimum power of 0.95 (α = 0.05) was ensured (Tables 2 and 3). Frequency of gene flow was highest at the closest distance (0.1 m) and declined at increasing distances from the pollen source. Averaged across eight directions (N, S, E, W, NE, NW, SE, and SW), gene flow frequency was 0.54 and 0.38 at 0.1 m from the pollen source in 2013 and 2014, respectively (Tables 2 and 3). Mean PMGF frequency was recorded ≤0.10 at a distance ≥35 m.
PMGF was not similar in different directional blocks of pollen-receptors or between years. The Akaike’s Information Criterion (AIC)-based model selection procedure [equation (5)] revealed that the inclusion of directions in the final model provided a better estimation for gene flow than the hourly measurements of wind parameters. The ΔAIC (difference in AICs) values between the top model and rest of the models were high (≥398.0), showing the efficacy of the top model for predicting PMGF in common waterhemp (Table S3). Gene flow in common waterhemp declined sharply with increasing distances from the pollen source (Figs 2 and 3), following a double exponential decay model [equation (6)] where the intercepts (β2) and decay rates (γ2) for the second instance varied with different directions and years (see coefficient estimations in Table 4). Pearson’s chi-square test showed a good fit of the model for all directions and years, where the null hypothesis (H0, the observed and predicted frequency of gene flow are the same) cannot be rejected due to a P-value ≥ 0.05.
The gene flow frequencies at 0 m distance were 0.77 and 0.69 in 2013 and 2014, respectively. Gene flow data from 0 m (GS plants planted inside the pollen-donor block) did not include any directions; therefore, could not be included in modeling. However, the final model [equation (6)] has predicted the PMGF frequency at 0 m for each direction and year, which was plotted later along with the original (observed) gene flow frequencies (Fig. 4). In 2013, the model-predicted frequencies (at 0 m) in the N and NW directions were similar to the observed frequency, and in 2014, the gene flow frequency in N direction corresponded with the observed frequency at 0 m.
The predicted distances where gene flow declined by 50% (O50) ranged from 0.6 to 2.4 m in 2013 and from 0.8 to 1.6 m in 2014 (Table 5) depending on the direction being investigated. The maximum distance at which 90% reduction (O90) in the frequency of gene flow was predicted, was 58.3 m in the S direction in 2013 and 87.6 m in the NW direction in 2014. The variability in gene flow increased with increasing distances from the pollen source; therefore, a broad range of O90 values were observed along with wider confidence intervals compared to the O50 values.
Mechanism and Inheritance of Glyphosate Resistance
To determine the mechanism and inheritance of glyphosate resistance, genomic DNA was extracted from young leaf tissues collected from GR and GS parents, F1, and an open-pollinated pseudo-F2 population. EPSPS gene copy number analysis revealed that gene amplification (and the assumed overproduction of EPSPS protein39) was the mechanism of glyphosate resistance in the GR common waterhemp parent used in the PMGF study. The mean relative copy number in the GR biotype was 5.3 (±2.2, standard deviation) (Fig. 5). As expected, the GS parent biotype had, on average, one EPSPS gene copy (mean = 1.0 ± 0.2). Plants with relative EPSPS gene copy number of >1.4 (the threshold copy number) were considered to be GR individuals44.
Seeds of F1 progeny (GS × GR) were directly taken from the field study (gene flow) and individuals surviving glyphosate application were selected for genomic DNA analysis. Similar to the mechanism of glyphosate resistance in the GR parent, EPSPS gene amplification was observed in 92% of the surviving individuals in the F1 progeny. For the GR parent, the maximum number of individuals was observed near the median value (5.1) of the relative EPSPS copy number, whereas the outcrossing between GS and GR parents resulted in a greater number of plants in F1 with a 2.0 to 4.0 EPSPS copy number (Fig. 5). EPSPS gene amplification was also observed in the pseudo-F2 population of common waterhemp with a mean relative EPSPS gene copy number of 6.6 ± 2.6 (Fig. 5). It is concluded that the EPSPS gene amplification is heritable in common waterhemp and can be transferred via pollen movement under field conditions. The pseudo-F2 population of common waterhemp has segregated for both relative EPSPS gene copy number and glyphosate resistance; however, the Chi-square test showed that the segregation did not agree with the null hypothesis (Mendelian pattern of segregation for a single gene or locus), which means that F2 individuals did not segregate in the 3GR:1GS pattern (Table 6).
Combining EPSPS Gene Amplification with the PMGF from Phenotypic Assessment
Mean relative EPSPS copy numbers (4.9 ± 2.2) from F1 progeny were combined with the gene flow frequency (Tables 2 and 3) to predict an average EPSPS gene copy number at any given distance from the pollen source. It was assumed that all GS individuals had a similar EPSPS copy number (1.0 ± 0.2). A double exponential decay model was fit to the average copy number data at different distances from the pollen source. Average relative EPSPS gene copy number was predicted as 3.9 and 1.9 at the 0 and 10 m distances, respectively, using the equation:
where x is the distance from the pollen source. The prediction plot (Fig. 6) for the relative EPSPS gene copy number shows the mean relative copy number in the individuals (assumed to be the F1 progeny) sampled randomly at a given distance from the pollen source.
The PMGF estimated in this study represents hybridization (or, gene flow frequency) as a function of distance from the pollen source (i.e., actual gene flow), rather than measuring the deposition of pollen from the source to a particular distance (i.e., potential gene flow). Beckie and Hall45 noted that it is more relevant to construct a model that predicts the actual gene flow rather than the potential gene flow, especially to understand the transfer of herbicide-resistant traits via pollen movement. PMGF depends on the mating system and reproductive biology of the plant species: for example, in highly outcrossing species such as rigid ryegrass, long-distance (up to 3 km) PMGF has been reported19, whereas in self-pollinating species such as barnyardgrass and common lambsquarters, the PMGF was very low (<10%), even at closer distances from the pollen source15,16. In this study PMGF has been estimated in ideal conditions with no physical or geographic barriers, other vegetation, and no GS male plants in the pollen-receptor blocks, which may have provided more opportunities for gene flow to occur.
The indeterminate growth habit and pulsed flowering pattern of common waterhemp aided in a prolonged flowering period, which ensured a complete flowering synchrony in this study. Wu and Owen46 explained that the extended flowering period of common waterhemp is an ecological adaptation to avoid harsh weather conditions and increase the chances of reproductive success. Moreover, long-distance pollen dispersal requires longer pollen viability47 and it has been reported that the viability of common waterhemp pollen is about 120 h32 with possibility of pollen shed throughout the day48,49.
Gene flow declined exponentially with distance, which was due to the weaker wind near the leeward edge of the donor block, resulting maximum pollen deposition near the edge of the pollen-donor block. Du et al.50 suggested that the wind direction near the leeward edge could also be the reverse in some cases. Results of this study also indicated that the average gene flow frequency at 50 m (the farthest distance tested in this study) was ≥0.05 (or ≥5% outcrossing) and the exponential decay model predicted that 90% reduction in gene flow occurred between 10.0 m to 87.6 m from the pollen source, depending on the direction of the pollen-receptor block. Bagavathiannan and Norsworthy15 also reported that the average gene flow in barnyardgrass was highly variable among directions and years; therefore, it was important to develop a statistical model that incorporates the effects of direction and year in a single model. A novel statistical approach adopted in this study fulfilled our goal for modeling the PMGF in common waterhemp and can be adopted in future studies to detect PMGF from crops and herbicide-resistant weeds.
The frequency of gene flow never reached 1.00 (i.e., 100% outcrossing) in this study, though the localized GS pollen sources were removed. The presence of GS-F1 progeny collected from the pollen-receptor blocks might have occurred for several reasons. Apomictic seed production and the presence of partially monoecious plants have been reported in common waterhemp51, and we have observed apomictic seed production in female GS plants grown in the greenhouse, where a few plants produced a small number of seeds without any pollen source and a partially monoecious GS plant was detected during flowering. The GR common waterhemp parent used in this study were derived from a field-collected segregating population and <5% GS male plants were observed in this population; these plants may have contributed pollen to this study, explaining the presence of GS-F1 progeny collected from pollen-receptor blocks. Glyphosate resistance by enhanced EPSPS copy number seems to be a dominant trait, and therefore susceptible alleles persist in phenotypically resistant plants, which would also result in GS plants in F1 progeny. Finally, GS Amaranthus species including common waterhemp were present at a distant location from the experimental site, which could have transferred some pollen to the pollen-receptor blocks.
Mallory-Smith et al.52 outlined the step-by-step approach for conducting a PMGF study under field conditions, and noted that the phenotypic data of herbicide resistance can be considered as an excellent marker, though it must be supplemented with molecular or morphological markers to confirm gene flow. Relative copy number analysis showed that the most GR-F1 individuals had increased EPSPS gene copy number; however, 4 out of 48 F1 individuals surviving glyphosate application possessed a relative EPSPS gene copy number of <1.4, which suggested that an additional mechanism could contribute to glyphosate resistance in this biotype. In a common waterhemp biotype collected from Mississippi, Nandula et al.53 reported both reduced glyphosate translocation and Pro106Ser point mutation in the EPSPS gene as potential glyphosate resistance mechanisms. It is possible that the parent GR population used in this study included a small proportion of individuals carrying additional, unknown mechanism(s) of resistance that might have been inherited in the F1 progeny, though all the GR individuals selected randomly for genomic DNA analysis showed an increase (>2.0) in EPSPS gene copy number. As reported in Palmer amaranth41, the results from this study suggested that EPSPS gene amplification is also heritable in common waterhemp, though inheritance of EPSPS gene amplification did not follow the 3:1 segregation ratio in pseudo-F2 progeny. This can be explained by detecting the location of resistant allele(s) on the genome, which was not performed in this study. Recombination within the block of tandem duplication may cause GS individuals to become less frequent than expected, as a large number of individuals would inherit extra gene copies and therefore be GR. If the extra gene copies are at different locations around the genome, the probability of an F2 individual inheriting the susceptible genotype (only 1 copy) is less than 25%, which would result in fewer GS individuals than expected.
Estimation of EPSPS gene amplification along with the prediction of gene flow provided strong evidence for the pollen-mediated transfer of the glyphosate resistant trait in common waterhemp. Here, the assessment of elevated EPSPS copy number was used not only to determine the mechanism of glyphosate resistance but also to confirm inheritance in subsequent progenies. However, EPSPS gene amplification could also be used as a molecular marker (instead of growing plants and applying herbicides for screening herbicide resistance) to detect gene flow in other important weed species (such as Italian ryegrass [Lolium multiflorum Lam.], kochia Palmer amaranth, ripgut brome [Bromus diandrus Roth.], etc.) where increased EPSPS copy number has been reported to confer glyphosate resistance54.
Results of this study indicated that the glyphosate-resistant trait in common waterhemp is highly mobile and its dispersal is possible via pollen movement depending on the distance from the resistant plants, the wind speed, and the wind direction. Therefore, the mobility of resistant alleles across farm enterprises should be addressed for effective management of this problem weed. Management strategies widely adapted by growers are mostly focused on preventing or delaying resistance evolution over a small area rather than preventing the large-scale movement of herbicide-resistance traits55. Field scouting in summer is essential for identifying patches of escaped common waterhemp plants and their management is necessary to prevent PMGF and the spread of resistant alleles. Before pollination, it is essential to identify the male and female common waterhemp plants, so that the removal of female common waterhemp plants, whether mechanically or physically, will reduce the chances of both PMGF and the spreading of herbicide resistance via seed dispersal.
This is the first report describing PMGF from glyphosate-resistant to -susceptible common waterhemp under field conditions. The results of this study addressed the rapid spread of GR common waterhemp in the Midwestern United States and encourage the re-evaluation of management strategies for this troublesome weed species. Being a small-seeded broadleaf weed species, seed-mediated gene flow could play an important role in the dispersal of herbicide-resistant alleles in common waterhemp, as its seeds can also be dispersed by wind, water streams, or by the movement of tillage or harvesting implements. Contaminated crop seeds and manure can also play an important role in seed dispersal. Thus, future research should evaluate the potential for seed-mediated gene flow in common waterhemp. Modeling approach considered in this study to predict the PMGF can also be used for the risk assessment of various transgenic traits in different commercial crops.
The known GR and GS common waterhemp biotypes were collected from two eastern Nebraska counties (Lancaster and Clay County, respectively); and their sensitivity to glyphosate has been evaluated. The level of glyphosate resistance was estimated 32-fold in the GR common waterhemp biotype compared to the GS biotype31.
Seeds from GR and GS biotypes were germinated in the greenhouse using the procedure described by Sarangi et al.31. The seedlings were transplanted at the cotyledon stage into 72-celled plastic germination trays containing potting mix (Berger BM1 All-Purpose Mix, Berger Peat Moss Ltd., Saint-Modeste, Quebec, Canada) and one seedling per cell was maintained. Plants were kept in the greenhouse and maintained at a constant 28/24 °C day/night temperature, and supplemental light was provided by metal halide lamps at an intensity of 600 μmol photon m−2 s−1 to maintain a 16 hour photoperiod. Plants were supplied with adequate water and nutrients (Miracle-Gro Water Soluble All Purpose Plant Food, Scotts Miracle-Gro Products Inc., 14111 Scottslawn Road, Marysville, OH 43041). Common waterhemp plants were then transplanted into the field at 8 to 10 cm height.
Field experiments were conducted in 2013 and 2014 at South Central Agricultural Laboratory (40.58°N, 98.14°W) at the University of Nebraska-Lincoln. The soil texture at the experimental site was Crete silt loam (montmorillonitic, mesic, Pachic Argiustolls) consisting of 17% sand, 58% silt, 25% clay, 3% organic matter, and a pH of 6.5. The experimental field was under a center-pivot irrigation system and was irrigated when needed. Based on the soil test report, starter fertilizers were applied following the University of Nebraska-Lincoln’s recommendations for soybean56. Previous observations listed common lambsquarters, common waterhemp, green foxtail (Setaria viridis (L.) Beauv.), Palmer amaranth, and velvetleaf (Abutilon theophrasti Medicus) as the primary weed species present at the experimental site; however, there was no report or suspicion of glyphosate resistance in any of these weed species. Field preparations were begun in mid-May by implementing tillage using a tandem disk harrow followed by the application of micro-encapsulated acetochlor [Warrant®, 359 g active ingredient (ai) L−1, Monsanto Company, 800 N. Lindbergh Blvd., St. Louis, MO 63167] tank-mixed with glyphosate [Roundup PowerMax®, 540 g acid equivalent (ae) L−1, Monsanto Company, 800 N. Lindbergh Blvd., St. Louis, MO 63167] to control the early-season emergence of weeds, specifically Amaranthus sp.57. The experimental area and its surroundings (up to 50 m) were either hand-weeded or cultivated later in the season to keep the area free of any Amaranthus species or other weeds. The experiments were conducted under a non-crop situation and there were no physical barriers to obstruct gene flow.
Field experiments were conducted using a concentric donor-receptor design (i.e., a Nelder wheel) where the pollen-donors were surrounded by the pollen-receptors [as described by Jhala et al.43]. The common waterhemp biotype resistant to glyphosate served as the pollen-donor in this study and the GS plants served as the pollen-receptors. The experimental area was 80 m × 80 m, which also comprised a center circle (80 square m; 10 m diameter) for the pollen-donor block (Fig. 7). Approximately 550 GR common waterhemp plants were transplanted to the pollen-donor block in East-West and North-South directions in a crisscross pattern with a 0.3 m plant-to-plant distance. The transplanting was performed on the first week of June to simulate the typical growing period of common waterhemp in the Midwest for maximum growth and seed production under field conditions46.
The receptor area was divided into eight directional blocks (cardinal: N, S, E, and W; ordinal: NE, NW, SE, and SW) and twelve GS common waterhemp plants were transplanted at each of the thirteen specific distances (0.1, 0.5, 1, 3, 4, 5, 10, 15, 20, 25, 35 m for all cardinal and ordinal directions; and additional 45, 50 m only for the ordinal directions) from the pollen-donor block (Fig. 7). Twenty GS plants were also transplanted inside the pollen-donor area (considered as 0 m from the source) to simulate the worst-case scenario of GS plants surrounded by a dense population of GR common waterhemp. GS plants inside the pollen-donor area were marked carefully with flags and plastic tags for easy identification.
Flowering Period and Seed Harvesting
Male GS common waterhemp plants were visually detected prior to pollen shedding and removed from the pollen-receptor blocks to reduce pollen competition within the receptor blocks. Liu et al.32 reported that common waterhemp plants can frequently be pollinated by the localized pollen source, which may reduce the chances for long-distance pollen movement. The number of flowering plants (male plants from the pollen-donor and females from the pollen-receptor blocks) was recorded at 5 d intervals and the flowering synchrony was assessed for each direction using the equation:
where n is the total number of distances in direction i (i = N, S, E, W, NE, NW, SE, or SW), A% is the percentage of plants shedding pollen in the pollen-donor area, and Bj% is the percentage of female flowering plants at the jth observation (distance) in the pollen-receptor blocks at that specific time. When X = 1.0, that means perfect synchrony occurs between pollen-donor and -receptor. X > 1.0 shows that sufficient pollen from GR male plants was present to pollinate GS females, but X values as low as 0.5 were not considered a good synchrony. Season-flowering synchrony (%) was assessed as the number of days pollen-donor and -receptor flowered together (X > 0.8) divided by the total number of flowering days by pollen-receptor female plants, multiplied by 100. Any GS female plants that were late (after 90% of the pollen-donors have completed pollen shedding) in flowering were removed from the receptor blocks.
At maturity, the seedheads of four GS common waterhemp plants from each distance and direction were harvested separately and labeled. Seeds were collected when ≥75% seeds in a seedhead turned into dark-violet or black. The seeds were then cleaned thoroughly and stored separately in airtight polythene bags at 4 °C for 2 months to overcome their limited seed dormancy.
Hourly surface meteorological data were recorded by the Bowen ratio energy balance systems (BREBS) stations of the Nebraska Water and Energy Flux Measurement, Modeling, and Research Network (NEBFLUX) available at the South Central Agricultural Laboratory, Clay Center, NE58. Wind frequency, wind speed, and wind run data were used for modeling PMGF in common waterhemp. Other meteorological data such as temperature, humidity, and precipitation were also recorded, as these factors can affect pollen viability and dispersal59.
A power analysis reported by Jhala et al.43 using binomial probabilities was performed to determine the minimum sample size required to accept an outcome without losing the precision of the statistical tests (see SI materials and methods). Seeds collected from individual common waterhemp plants were germinated separately in the greenhouse and evaluated for glyphosate resistance. Plastic trays (51 cm × 38 cm × 10 cm) containing potting mix were used for growing the plants. A maximum of 200 plants were allowed per tray to ensure sufficient glyphosate coverage on the leaf surface. The putative hybrid plants were sprayed at an 8–10 cm height with 1.5× the recommended rate of glyphosate (Touchdown HiTech®, Syngenta Crop Protection, LLC, P.O. Box 18300, Greensboro, NC 27419-8300), where 1× = 1,050 g ae ha−1. The resistance screening was performed at the 1.5 × rate (1,575 g ae ha−1) of glyphosate to obtain more consistency in the response of common waterhemp plants, to ensure the complete control of any GS plant present, and to assure the survival of any GR plant (as the ED50 value, the effective dose to control 50% plant population, for GR parent plants was higher than the 1.5× rate of glyphosate; Fig. S3). The number of seedlings surviving glyphosate treatment was recorded at 21 days after herbicide application and the frequency of gene flow at each distance/direction was calculated using the equation:
A novel statistical procedure was used in this study for modeling the PMGF. Rapid reduction in PMGF with increasing distances from the pollen source can be explained by the leptokurtic curve (high probability distribution in the tail) rather than by normal distribution52,60. The exponential decay, or the exponential power functions, are widely reported statistical models that describe PMGF better45.
In this study, all statistical analyses were carried out in R Version 3.2.261. Frequency of gene flow data from all distances and directions and from both years were subjected to nonlinear regression using Generalized Nonlinear Models (package gnm). The frequency of gene flow data followed a binomial distribution, as there were two possible outcomes (dead or alive seedlings) from the resistance screening procedure. Unlike the Gaussian distribution, both mean and variance are dependent on the underlying probability, pi, in a binomial distribution. Compared to the nonlinear least square (nls) function used in several studies (e.g., Pluess et al.62), gnm has two notable advantages for modeling the PMGF: (i) responses with non-Gaussian distribution can be fitted, and (ii) it is a more convenient way to represent a model with a large number of parameters by symbolic model specification63.
In binomial distribution, the probability of success (pi) is a function of the covariate x (here, x is the distance from the pollen source) that can take any real value, but the pi should range between 0 and 1 (0 ≤ pi ≤ 1). Therefore, it was important to transform the frequency of gene flow data to remove the range and floor restrictions; the Logit, or log-odds, link function serves this purpose64:
The frequency of gene flow data from both years were described using the exponential decay function in which the independent variables were distance from the pollen source, direction of the pollen-receptor blocks, average wind speed, wind frequency, wind run, and year. Therefore, 62 possible models were constructed and subjected to the test for best fit (Table S3).
The Akaike’s Information Criterion (AIC) was used for the model comparison and selection of the best model using the equation65:
where LL is the log-likelihood function for the models and K is the number of parameters estimated. The model with the lowest AIC value was considered as the “top model” or best candidate model tested66.
A double exponential decay model [equation (6)] where the frequency of gene flow varied with the distance from the pollen source, the direction of the pollen-receptor blocks, and the year provided the best fit to the data (Table S3):
where pi is the frequency of gene flow of the ith observation; β0 is the overall intercept; β1, and β2 are the intercepts for the first and second instances, respectively; and γ1, and γ2 are the decay rates where γ1 > γ2. Here, β2 and γ2 vary with the direction and the year.
The distances where the frequency of gene flow was reduced by 50% (O50) and 90% (O90) of the predicted frequency at 0 m were estimated from the final model [equation (6)].
Model Goodness of Fit
The difference between observed and fitted values for the final model was measured. Model goodness of fit was estimated by Pearson’s chi-squared statistic using the equation67:
where the sum of the squared difference between yi (observed values) and (fitted values for the ith group of observations) was divided by the variance of yi that was μi (ni − μi)/ni (with μi estimated using ) and ni is the sample size for ith group. The degree of freedom for Pearson’s chi-squared statistic was n − k − 1, where n was the total number of groups and k was the number of parameters.
Mechanism and Inheritance of Glyphosate Resistance
Common waterhemp individuals that survived the resistance screening procedure were considered as hybrids (GS × GR; i.e., F1 progeny). Thirty healthy individuals from the F1 progeny were selected and transplanted into round plastic pots (20 cm diameter and 30 cm height) containing a 3:1 mixture of soil:potting mix and grown in the greenhouses at the University of Nebraska-Lincoln. Transplanted individuals were again sprayed with glyphosate at 790 g ae ha−1 (0.75× the recommended rate) to confirm resistance. Low-level plant injury (chlorosis, stunting, frequent branching, etc.) was observed in less than 50% of the transplanted F1 individuals. No mortality was observed, indicating that no susceptible individuals were present (ED90 for the GS biotype was 659 g ae ha−1; Fig. S3). Ten F1 individuals were selected based on their similarities in morphology and flowering initiation time and kept for open pollination in the greenhouse. Seeds were collected at maturity and designated as pseudo-F2, as the dioecious nature of common waterhemp prohibits self-pollination of F1 plants to form true-F2 progeny.
Seeds from the parent biotypes (GR and GS) and the open-pollinated pseudo-F2 were germinated in 72-celled germination trays containing potting mix. Emerged seedlings were transplanted at 2 cm height into square plastic pots (10 × 10 × 12 cm). A 50 mg sample of young meristematic leaf tissue was collected from each individual (25 individuals each from the GR and GS biotypes, and 44 individuals from the F2 progeny) and were immediately frozen in liquid nitrogen. Individuals were then treated with glyphosate (at the rate of 1,575 g ae ha−1; 1.5× the recommended rate) to compare the genomic EPSPS copy number data with the greenhouse visual control. Leaf tissue samples were also collected from 48 individuals (3 randomly selected distances × 8 directions × 2 years from gene flow study) of the F1 progeny that survived the resistance screening process.
DNA extraction and analysis were performed in the Molecular Weed Science Lab at Colorado State University. Leaf tissue samples were ground to a fine powder using a Qiagen TissueLyser II (Qiagen Inc., Valencia, CA 91355) and a metal bead for 1 min at 30 oscillations per second. Genomic DNA extraction was performed on the leaf tissue using the modified cetyltrimethylammonium bromide (CTAB) method described by Doyle68. The concentration and purity of the DNA was measured using a spectrophotometer (Nano Drop 2000 Spectrophotometer, Thermo Fisher Scientific, Wilmington, DE 19810) and each sample was diluted to 10 ng μL−1.
EPSPS Genomic Copy Number
Samples were tested for EPSPS gene copy number relative to a one-copy reference gene, CPS (which encodes the large subunit of carbamoylphosphate synthase [EC 188.8.131.52] and is not associated with any known herbicide resistance)69. An 81 base pair (bp) fragment of the EPSPS gene was amplified using the forward primer EPSPS_FP1 (5′-GGTTGTGGTGGTCTGTTTCC-3′) and the reverse primer EPSPS_RP1 (5′-CATCGCTGTTCCTGCATTTC-3′). The CPS forward and reverse primers were as follows to amplify a 78 bp fragment: CPS_FP1 (5′-ATTGATGCTGCCGAGGATAG-3′) and CPS_RP1 (5′-GATGCCTCCCTTAGGTTGTTC-3′).
For both CPS and EPSPS, genomic DNA templates (10 ng) were amplified in a 25-μL reaction volume containing forward and reverse primers (400 nM final concentration) and Quanta 2X PerfeCTa qPCR SYBR Green FastMix (Quantabio, Beverly, MA), using a real-time qPCR thermal cycler (Bio-Rad) protocol. The qPCR reactions were heated to 95 °C for 30 s, followed by 40 cycles of 30 s at 95 °C and 30 s at 60 °C. Real-time data for fluorescence of the asymmetrical cyanine dye SYBR Green were recorded at the end of the amplification step in every cycle. A melt curve analysis of the products was performed by heating the PCR products from 60 to 95 °C, in 0.5 °C increments for 5 seconds and reading fluorescence at each step. Melt curve profiles were used to verify a single melting point, indicative of a single PCR product and lack of non-specific products. As a secondary check of amplification specificity, a 5 μL aliquot of the PCR product was run on 1% agarose gel to verify the presence of a single band (Fig. S4).
Relative quantification was conducted using the comparative cycle threshold (CT; ) methods described by Schmittgen and Livak70. Relative quantification of EPSPS for each sample was calculated using the equation:
Relative EPSPS copy number was expressed as . Each individual sample was run in triplicate to calculate the average increase in EPSPS copy number and standard deviation.
How to cite this article: Sarangi, D. et al. Pollen-mediated gene flow from glyphosate-resistant common waterhemp (Amaranthus rudis Sauer): consequences for the dispersal of resistance genes. Sci. Rep. 7, 44913; doi: 10.1038/srep44913 (2017).
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Endler, J. A. Geographic Variation, Speciation, and Clines. 20–29 (Princeton University Press, 1977).
Nei, M. & Nozawa, M. Roles of mutation and selection in speciation: From Hugo de Vries to the modern genomic era. Genome Biol. Evol. 3, 812–829 (2011).
Gressel, J. Dealing with transgene flow of crop protection traits from crops to their relatives. Pest Manag. Sci. 71, 658–667 (2015).
Andrews, C. A. Natural selection, genetic drift, and gene flow do not act in isolation in natural populations. Nat. Educ. Knowl. 3, 5 (2010).
Délye, C., Clément, J. A. J., Pernin, F., Chauvel, B. & Le Corre, V. High gene flow promotes the genetic homogeneity of arable weed populations at the landscape level. Basic Appl. Ecol. 11, 504–512 (2010).
Levin, D. A. Dispersal versus gene flow in plants. Ann. Missouri Bot. Gard. 68, 233–253 (1981).
Délye, C., Jasieniuk, M. & Le Corre, V. Deciphering the evolution of herbicide resistance in weeds. Trends Genet. 29, 649–658 (2013).
Jasieniuk, M., Brûlé-Babel, A. L. & Morrison, I. N. The evolution and genetics of herbicide resistance in weeds. Weed Sci. 44, 176–193 (1996).
Ellstrand, N. C. Gene flow by pollen: implications for plant conservation genetics. Oikos 63, 77–86 (1992).
Arrigo, N. et al. Gene flow between wheat and wild relatives: empirical evidence from Aegilops geniculate, Ae. neglecta and Ae. triuncialis . Evol. Appl. 4, 685–695 (2011).
Schmidt, M. & Bothma, G. Risk assessment for transgenic sorghum in Africa: Crop-to-crop gene flow in Sorghum bicolor (L.) Moench. Crop Sci. 46, 790–798 (2006).
Watrud, L. S. et al. Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proc. Natl. Acad. Sci. USA 101, 14533–14538 (2004).
Arnaud, J.-F., Viard, F., Delescluse, M. & Cuguen, J. Evidence for gene flow via seed dispersal from crop to wild relatives in Beta vulgaris (Chenopodiaceae): consequences for the release of genetically modified crop species with weedy lineages. Proc. R. Soc. Lond. B 270, 1565–1571 (2003).
Beckie, H. J., Warwick, S. I., Nair, H. & Séguin-Swartz, G. Gene flow in commercial fields of herbicide-resistant canola (Brassica napus). Ecol. Appl. 13, 1276–1294 (2003).
Bagavathiannan, M. V. & Norsworthy, J. K. Pollen-mediated transfer of herbicide resistance in Echinochloa crus-galli . Pest Manag. Sci. 70, 1425–1431 (2014).
Yerka, M. K., de Leon, N. & Stoltenberg, D. E. Pollen-mediated gene flow in common lambsquarters (Chenopodium album). Weed Sci. 60, 600–606 (2012).
Beckie, H. J., Blackshaw, R. E., Hall, L. M. & Johnson, E. N. Pollen- and seed-mediated gene flow in kochia (Kochia scoparia). Weed Sci. 64, 624–633 (2016).
Sosnoskie, L. M. et al. Pollen-mediated dispersal of glyphosate-resistance in Palmer amaranth under field conditions. Weed Sci. 60, 366–373 (2012).
Busi, R., Yu, Q., Barrett-Lennard, R. & Powles, S. Long distance pollen-mediated flow of herbicide resistance genes in Lolium rigidum . Theor. Appl. Genet. 117, 1281–1290 (2008).
Fénart, S., Austerlitz, F., Cuguen, J. & Arnaud, J. F. Long distance pollen-mediated gene flow at a landscape level: The weed beet as a case study. Mol. Ecol. 16, 3801–3813 (2007).
Murray, B. G., Morrison, I. N. & Friesen, L. F. Pollen-mediated gene flow in wild oat. Weed Sci. 50, 321–325 (2002).
Prince, J. M. et al. Benchmark study: I. Introduction, weed population, and management trends from the benchmark survey 2010. Weed Technol. 26, 525–530 (2012).
Mosyakin, S. L. & Robertson, K. R. New infrageneric taxa and combinations in Amaranthus (Amaranthaceae). Ann. Bot. Fenn. 33, 275–281 (1996).
Steckel, L. E. The dioecious Amaranthus spp.: Here to stay. Weed Technol. 21, 567–570 (2007).
Sauer, J. Recent migration and evolution of the dioecious amaranths. Evolution 11, 11–31 (1957).
Trucco, F., Tatum, T., Rayburn, A. L. & Tranel, P. J. Out of the swamp: Unidirectional hybridization with weedy species may explain the prevalence of Amaranthus tuberculatus as a weed. New Phytol. 184, 819–827 (2009).
Sarangi, D., Irmak, S., Lindquist, J. L., Knezevic, S. Z. & Jhala, A. J. Effect of water stress on the growth and fecundity of common waterhemp (Amaranthus rudis). Weed Sci. 64, 42–52 (2016).
Nordby, D., Hartzler, B. & Bradley, K. Biology and Management of Waterhemp. GWC 13, 3 (Purdue Extension, 2007).
Legleiter, T. R. & Bradley, K. W. Glyphosate and multiple herbicide resistance in common waterhemp (Amaranthus rudis) populations from Missouri. Weed Sci. 56, 582–587 (2008).
Heap, I. Herbicide resistant tall waterhemp globally. International Survey of Herbicide Resistant Weeds . (Available at: http://weedscience.org/summary/species.aspx?WeedID=219; Accessed on: 17 September, 2016).
Sarangi, D. et al. Confirmation and control of glyphosate-resistant common waterhemp (Amaranthus rudis) in Nebraska. Weed Technol. 29, 82–92 (2015).
Liu, J., Davis, A. S. & Tranel, P. J. Pollen biology and dispersal dynamics in waterhemp (Amaranthus tuberculatus). Weed Sci. 60, 416–422 (2012).
Borsch, T. Pollen types in the Amaranthaceae: Morphology and evolutionary significance. Grana 37, 129–142 (1998).
Franssen, A. S., Skinner, D. Z., Al-Khatib, K. & Horak, M. J. Pollen morphological differences in Amaranthus species and interspecific hybrids. Weed Sci. 49, 732–737 (2001).
Smits, A. J. & Ogg, S. Aerodynamics of the golf ball. Biomedical Engineering Principles in Sports. (eds Hung, G. K., Pallis, J. M. ) 3–27 (Springer-Science + Business Media, LLC, 2004).
Costea, M., Weaver, S. E. & Tardif, F. J. The biology of invasive alien plants in Canada. 3. Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea & Tardif. Can. J. Pl. Sci. 85, 507–522 (2005).
Raynor, G. S., Ogden, E. C. & Hayes, J. V. Dispersion and deposition of corn pollen from experimental sources. Agron. J. 64, 420–427 (1972).
Chatham, L. A. et al. A multistate study of the association between glyphosate resistance and EPSPS gene amplification in waterhemp (Amaranthus tuberculatus). Weed Sci. 63, 569–577 (2015).
Lorentz, L. et al. Characterization of glyphosate resistance in Amaranthus tuberculatus populations. J. Agric. Food Chem. 62, 8134–8142 (2014).
Dillon, A. J. Relationship between EPSPS Copy Number, Expression, and Level of Resistance to Glyphosate in Common Waterhemp (Amaranthus rudis) from Kansas. 51 (Kansas State University, 2015).
Gaines, T. A. et al. Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc. Natl. Acad. Sci. USA 107, 1029–1034 (2010).
Schmidt, J. J., Pedersen, J. F., Bernards, M. L. & Lindquist, J. L. Rate of shattercane × sorghum hybridization in situ . Crop Sci. 53, 1677–1685 (2013).
Jhala, A. J., Bhatt, H., Topinka, K. & Hall, L. M. Pollen-mediated gene flow in flax (Linum usitatissimum L.): Can genetically engineered and organic flax coexist? Heredity 106, 557–566 (2011).
Chatham, L. A. et al. EPSPS gene amplification is present in the majority of glyphosate-resistant Illinois waterhemp (Amaranthus tuberculatus) populations. Weed Technol. 29, 48–55 (2015).
Beckie, H. J. & Hall, L. M. Simple to complex: Modelling crop pollen-mediated gene flow. Plant Sci. 175, 615–628 (2008).
Wu, C. & Owen, M. D. K. When is the best time to emerge: Reproductive phenology and success of natural common waterhemp (Amaranthus rudis) cohorts in the midwest United States? Weed Sci. 62, 107–117 (2014).
Dafni, A. & Firmage, D. Pollen viability and longevity: practical, ecological and evolutionary implications. Pollen and Pollination. (eds Dafni, A., Hesse, M., Pacini, E. ) 113–132 (Springer-Verlag Wien, 22000).
Bell, M. S. & Tranel, P. J. Time requirement from pollination to seed maturity in waterhemp (Amaranthus tuberculatus). Weed Sci. 58, 167–173 (2010).
Rodríguez, A. F. M., Palacios, I. S., Molina, R. T., Corchero, A. M. & Muñoz, J. T. Dispersal of Amaranthaceae and Chenopodiaceae pollen in the atmosphere of Extremadura (SW Spain). Grana 39, 56–62 (2000).
Du, M., Kawashima, Y., Matsuo, K., Yonemura, S. & Inoue, S. Simulation of the effect of a cornfield on wind and on pollen deposition. MODSIM 2001. (ed. Ghassenmi, F. ) 899–903 (The Modelling and Simulation Society of Australia and New Zealand Inc., 2001).
Franssen, A. S., Skinner, D. Z., Al-khatib, K., Horak, M. J. & Kulakow, P. A. Interspecific hybridization and gene flow of ALS resistance in Amaranthus species. Weed Sci. 49, 598–606 (2001).
Mallory-Smith, C., Hall, L. M. & Burgos, N. R. Experimental methods to study gene flow. Weed Sci. 63, 12–22 (2015).
Nandula, V. K., Ray, J. D., Ribeiro, D. N., Pan, Z. & Reddy, K. N. Glyphosate resistance in tall waterhemp (Amaranthus tuberculatus) from Mississippi is due to both altered target-site and nontarget-site mechanisms. Weed Sci. 61, 374–383 (2013).
Sammons, R. D. & Gaines, T. A. Glyphosate resistance: State of knowledge. Pest Manag. Sci. 70, 1367–1377 (2014).
Harker, K. N. & O’Donovan, J. T. Recent Weed Control, Weed Management, and Integrated Weed Management. Weed Technol. 27, 1–11 (2013).
Shaver, T. M., Ferguson, R. B., Penas, E. J. & Stevans, W. B. Soybeans. Nutrient Management for Agronomic Crops in Nebraska. (ed. Shaver, T. M. ) EC 155, 116–120 (The University of Nebraska Institute of Agricultural and Natural Resources, 2014).
Jhala, A. J., Malik, M. S. & Wills, J. B. Weed control and crop tolerance of micro-encapsulated acetochlor applied sequentially in glyphosate-resistant soybean. Can. J. Pl. Sci. 95, 973–981 (2015).
Irmak, S. Nebraska Water and Energy Flux Measurement, Modeling, and Research Network (NEBFLUX). Trans. ASABE 53, 1097–1115 (2010).
Shivanna, K. R., Linskens, H. F. & Cresti, M. Pollen viability and pollen vigor. Theor. Appl. Genet. 81, 38–42 (1991).
Petrovskii, S., Morozov, A. & Li B.-L. On a possible origin of the fat-tailed dispersal in population dynamics. Ecol. Complex 5, 146–150 (2008).
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. (Available at: https://www.R-project.org; Accessed on: February 3, 2016).
Pluess, A. R. et al. Short distance pollen movement in a wind-pollinated tree, Quercus lobata (Fagaceae). For Ecol. Manage. 258, 735–744 (2009).
Turner, H. & Firth, D. Generalized Nonlinear Models in R: An Overview of the gnm. 24 (Department of Statistics, University of Warwick, 2015).
Cramer, J. S. Logit Models from Economics and Other Fields. 9–32 (Cambridge University Press, 2003).
Akaike, H. A new look at the statistical model identification. IEEE T . Automat. Contr. 19, 716–723 (1974).
Collett, D. Texts in Statistical Science: Modelling Survival Data in Medical Research. 2nd edn, 81 (Chapman & Hall/CRC, 2003).
Rodríguez, G. Logit Models for Binary Data. Princeton University. (Available at: http://data.princeton.edu/wws509/notes; Accessed on: 25 January, 2016).
Doyle, J. DNA protocols for plants. Molecular Techniques in Taxonomy. (eds Hewitt, G. M., Johnston, A. W. B., Young, J. P. W. ) 283–293 (Springer-Verlag, 1991).
Ma, R. et al. Distinct detoxification mechanisms confers resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol. 163, 363–377 (2013).
Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative CT method. Nat. Protoc. 3, 1101–1108 (2008).
We thank the Indian Council of Agricultural Research (ICAR), New Delhi, India for partial financial support to Debalin Sarangi for this study. We also appreciate the help of Bradley Meusch, Ian Rogers, Irvin Schleufer, Katy McIntyre, Mason Adams, Parminder Chahal, Simranpreet Kaur, and Zahoor Ganie in field, greenhouse, and laboratory experiments. We appreciate partial financial support of USDA-NIFA Hatch project # NEB 22-365 and USDA-NIFA-Nebraska Extension Implementation Program project # NEBN-09-504.
The authors declare no competing financial interests.
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Sarangi, D., Tyre, A., Patterson, E. et al. Pollen-mediated gene flow from glyphosate-resistant common waterhemp (Amaranthus rudis Sauer): consequences for the dispersal of resistance genes. Sci Rep 7, 44913 (2017). https://doi.org/10.1038/srep44913
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