Figure 2: Effects of VCP modulators on retinal cells in an ischemic retinal injury model. | Scientific Reports

Figure 2: Effects of VCP modulators on retinal cells in an ischemic retinal injury model.

From: KUS121, a VCP modulator, attenuates ischemic retinal cell death via suppressing endoplasmic reticulum stress

Figure 2

(a,b) RGC numbers in the ischemic retina using SLO imaging. (a) Representative images from animals treated with KUS121 or not 28 days after ischemia. Bar = 500 μm. (b) Longitudinal changes in RGCs counted within the red boxes in a in control (n = 11, triangles) and KUS121-treated (n = 11, circles) groups. Error bars indicate SD. **P < 0.01 and ***P < 0.005 vs. control (Student’s t test). (c and d) GFP-positive RGC numbers in flat-mounted retinas treated with KUS121 or not 14 days after ischemia. (c) Representative images. (d) GFP-positive RGC numbers counted within the red boxes in c in control (n = 8) and KUS121-treated (n = 10) groups. Error bars indicate SE. *P < 0.05 vs. control (Student’s t test). (eg) Retinal section analysis 14 days after ischemic retinal injury. (e) Immunohistochemical images of retinal sections stained with HPC-1 and TOTO-3. Bar = 50 μm. (f) Inner nuclear layer thickness (each group, n = 8). *P < 0.05 vs. control (Student’s t test). (g) Numbers of HPC-1–positive cells in the inner nuclear layer (corresponding to amacrine cells) (each group, n = 8). *P < 0.05 vs. control (Aspin-Welch t test).

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