Tissue annotation by FTIR imaging for lung tumours and thoracal tumours is illustrated. The classifiers for lung tumours and thoracal tumours (shaded in grey) were previously published and reprinted from Großerueschkamp et al. Analyst 201536. In a first classifier, the pathologic regions were identified. In a second classifier, the different tumour classes of the pathologic region are differentiated. Finally in the third classifier, a differential diagnosis of the tumour subtypes is provided. The DMM subtype classifier was trained especially for this study based on newly acquired samples. This is an important step establishing a differential and predictive thoracal tissue classifier.