Figure 6 : Flow cytometric analysis of surface activation markers on human monocytes stimulated with masked and non-masked LPS.

From: Biological Activity of Masked Endotoxin

Figure 6

1 × 105 monocytes were stimulated as indicated. After 24 h of incubation, cells were stained for the expression of CD40, CD80, CD83, and CD86 and subsequently analysed by flow cytometry. Median fluorescence intensity values of 1 × 104 cells were recorded for each sample. Mean and SD of 5 independent experiments are shown. For statistical analysis within each group, ANOVA with a Dunnett’s post-test was performed. For comparisons between different groups, ANOVA with a Tukey post-test was performed. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s. not significant.