Figure 6 : In human GC-induced osteonecrosis of the femoral head, trabecular bone matrix is hypermineralized and OCYs are viable.

From: Glucocorticoid suppression of osteocyte perilacunar remodeling is associated with subchondral bone degeneration in osteonecrosis

Figure 6

Three-dimensional reconstructions of a HR-pQCT scan of a cadaveric human femoral head from a donor without joint disease exhibits a smooth surface (A), organized trabecular microarchitecture and an intact cortical shell (B), white inset box signifies ROI in (G and J). Human osteonecrotic femoral head displaying fragmented surface (D), disorganized trabecular microarchitecture, and a thin cortical shell (E), red inset box signifies ROI in (H,K and N). Blue inset box signifies ROI in (I,L and M). Gross differences were apparent between Heidenhain’s trichrome-stained histologic sections of cadaveric (C) and osteonecrotic (F) femoral heads (Scale bar = 10 mm for (A–F). Hematoxylin and eosin-stained sections of cadaveric trabecular bone (G,J) and trabecular bone near (H,K) and far from (I,L) the osteonecrotic lesion shows lacunae occupied with OCYs in each condition (Scale bar = 200 μm in (G,H,I) Scale bar = 20 μm in (J,K,L). Quantitative (M) and qualitative (N,O) analysis of mineral density shows that trabeculae in the sclerotic zone of osteonecrotic lesions (N) are hypermineralized, relative to those distant from the lesion (O). In M, mineralization is normalized to percentage of bone volume within normal and osteonecrotic regions of interest (n = 3, see methods for details).