Figure 3 : GCs repress hallmark outcomes of perilacunar remodeling in cortical bone.

From: Glucocorticoid suppression of osteocyte perilacunar remodeling is associated with subchondral bone degeneration in osteonecrosis

Figure 3

Immunohistochemistry for MMP-13 protein in placebo and prednisolone-treated trabecular (A,B) and cortical (C,D) compartments after 21 days treatment showing diminished staining in cortical bone due to GC treatment (scale bar = 20 μm). (E) Quantification of percent MMP-13-postiive OCYs normalized to total OCYs in cortical bone. For all analyses, 5 high-powered fields per mouse, 4 mice per group (Mean ± SEM, *p-value ≤ 0.05 vs. placebo control). Representative picrosirius-red stained histological sections of the same bones viewed using polarized light microscopy show GC-dependent differences in collagen orientation in trabecular (F,G) and cortical (H,I) compartments (scale bar = 50 μm), shown quantitatively for cortical bone in J. (J) Orientation J was used to determine the range of orientation degree for collagen and graphed as a composite average across multiple specimens per group. Each line represents the average ± SEM, *p-value ≤ 0.05 vs. placebo control peak orientation value.