Figure 2 : GCs induce a defect in osteocyte function independently of apoptosis in cortical bone.

From: Glucocorticoid suppression of osteocyte perilacunar remodeling is associated with subchondral bone degeneration in osteonecrosis

Figure 2

Histological sections of femurs stained for TUNEL activity (cells stained green, white arrows) and counterstained with Hoechst (scale bar = 50 um) in proximal trabecular bone (A–D) and mid-shaft cortical bone (G–J) after 7 and 21 days treatment with placebo and prednisolone. (E) Low magnification image of trabecular bone from Day 7 Prednisolone treated (scale bar = 200 um), and corresponds to section used for high magnification image shown in (B). (F and L), Average TUNEL-positive osteocytes over total osteocytes from 5 high powered fields per specimen, 4 specimens per group (Mean ± SEM, *p-value ≤ 0.05). (K) Positive control trabecular bone section treated with DNase (scale bar = 200 um). MLO-Y4 cells were treated with vehicle (Veh, PBS) or Dexamethasone (Dex) alone or after 30 minute pretreatment with Caspase-3 inhibitor, DEVD (1 μm). mRNA was isolated after 3 hours (M) and 24 hours (N) treatment and MMP13 expression measured by real-time quantitative PCR (n = 3 independent experiments, 3 biological replicates each). All mRNA levels were normalized to GAPDH mRNA levels and vehicle treated expression (*p-value ≤ 0.05).