Figure 1 : Thyroid disrupting activity of individual chemicals assessed with XETA.

From: Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos

Figure 1

Screening of thyroid disrupting activity of molecules measured in humans with the Xenopus Embryonic Thyroid Assay (XETA), based on the quantification of fluorescence-using the transgenic TH/bZip-eGFP e.g. [(Tg(thibz:eGFP)] line. Fifteen compounds were tested at different concentrations in presence of T3 5 × 10−9 M for 72 h. Scattered plots are shown with mean +/− SD of three to five independent experiments pooled (normalised on T3 to 100%). The GFP fluorescence in whole tadpoles (mainly heads) was measured and quantified after 72 h exposure. (a) Phenolic compounds: BPA, Triclosan and Benzophenone-3. (b) Phthalates: DBP and DEHP. (c) Organochlorine pesticides: HCB and 4′4-DDE. (d) Perfluorinated compounds: PFOA and PFOS. (e) Polyaromatic hydrocarbon: 2-Naphtol. (f) Halogenated compounds: Sodium perchlorate, PCB-153 and BDE-209. (g) Metals: Methylmercury and Lead chloride. Red arrowheads indicate concentrations of chemicals used in mix 1x (Table S1). Statistics were done with non-parametric Kruskal-Wallis test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Hashes (###) represent p < 0.001, T3 vs Control using column to column comparison (non parametric Mann Whitney).