Figure 1

From: Three-dimensional mouse brain cytoarchitecture revealed by laboratory-based x-ray phase-contrast tomography

Figure 1

Laboratory setup (a) Schematic of the setup, in which X-rays are generated by a liquid-metal jet microfocus source. Depending on the ratio between the source-to-object and object-to-detector distances, it is either implemented in a cone-beam geometry or an inverse geometry imaging mode. (b) Image of an absorbing test pattern with 800 nm lines and spaces, together with the corresponding line profile along the structure (width: 50 px). (c) Close-up of a detached (mouse) cerebellar vermis and mounting of the sample in a pipette tip. (d) Typical empty-beam corrected projection of the sample in inverse geometry, with a line profile (width: 5 px) along an edge to illustrate the edge-enhancement effect. For a better signal-to-noise ratio, the projection has been resampled by a factor 2. (e) Reconstruction of the intensity distribution using the BAC algorithm. The line profile shows the advantage of the phase retrieval as the edge-enhancement is removed while simultaneously the signal-to-noise ratio increases. Scalebars: (c) 1 mm and (d,e) 250 μm.