Whereas Huntington’s disease (HD) is unequivocally a neurological disorder, a critical mass of emerging studies highlights the occurrence of peripheral pathology like cardiovascular defects in both animal models and humans. The overt impairment in cardiac function is normally expected to be associated with peripheral vascular dysfunction, however whether this assumption is reasonable or not in HD is still unknown. In this study we functionally characterized the vascular system in R6/2 mouse model (line 160 CAG), which recapitulates several features of human pathology including cardiac disease. Vascular reactivity in different arterial districts was determined by wire myography in symptomatic R6/2 mice and age-matched wild type (WT) littermates. Disease stage was assessed by using well-validated behavioural tests like rotarod and horizontal ladder task. Surprisingly, no signs of vascular dysfunction were detectable in symptomatic mice and no link with motor phenotype was found.
Huntington’s disease (HD) is one of the most common non-curable rare diseases characterized primarily by a progressive loss of cognitive and motor function leading to severe disability and death in affected patients1. Expansion of the trinucleotide repeat (CAG) within the huntingtin (HTT) gene is recognized as the major cause2 and its length is known to profoundly influence age at onset and disease phenotype3,4. Whereas HD is unequivocally a neurological disorder, there is a critical mass of emerging studies suggesting peripheral pathology as an important factor that might significantly contribute to the overall presentation and progression of the disease.
Interestingly, multiple epidemiological studies report evidence of heart pathology in HD and describe cardiac failure as one of the more common causes of death among disease patients5,6. Subtle abnormalities of autonomic control of the cardiovascular system in HD have already been reported at pre-symptomatic and early stage7,8 and described to gradually progress in range and magnitude as the disease advances9. Similar dysfunctional cardiac phenotype has been observed also in pre-clinical HD settings10,11,12, however still much is needed to fully understand whether there exists any direct association with peripheral vascular function. Despite the available studies examining this possible correlation in HD, there is currently insufficient evidence to make a definitive conclusion.
While alterations in the structure of vascular network have clearly been implicated in brain pathology in HD13, definitive evidence of vascular homeostasis is still lacking.
Aside from few studies reporting only partial evidence of deranged peripheral vascular function in some of the available HD models14,15, no comprehensive study that systematically investigated the functional vascular reactivity has been conducted so far.
To this regard, here we sought to provide a more complete profile of vascular function and contractile properties of either resistance or capacitance vessels in both central and peripheral districts in R6/2 mouse model (160 CAG), the better characterized and the most used model when studying cardiac function in HD11,12,13,15, which recapitulates several features of motor and behavioural phenotype of early human pathology16,17.
In this study, our thorough investigation of symptomatic 12 week old R6/2 mice has led to the first full characterization of vascular function in this model, in which we could not detect any vascular dysfunction or molecular defects in possible related signalling pathways like the one involving the synthesis of Nitric Oxide (NO), whose dysfunction has been previously hypothesized to pre-date the disease manifestation in HD models15.
Transgenic HD R6/2 line, expressing exon 1 of the human huntingtin gene carrying approximately 160 +/− 5 GAC repeat expansions, was originally purchased from Jackson Laboratories (Bar Harbor, ME, USA) and the colony was maintained by breeding heterozygous R6/2 males with wild-type (WT) females from their background strain (B6CBA-Tg(HDexon)62oGpb/J) in the animal facility at IRCCS Neuromed. Genotyping was confirmed by PCR and performed at 3 weeks of age to determine study groups. Mixed gender F1 mouse generation was used in this study. Animals were housed in polycarbonate cages (15 × 23 × 17 cm) provided with a mouse house and aspen bedding and maintained under temperature (22–24 °C) and humidity-controlled (55%) conditions. Food and water were provided ad libitum.
All efforts were made to minimize the number of animals used and their suffering. All the experiments reported in this study were performed on the same animal groups. All animal procedures were conformed to the guidelines for the Care and Use of Laboratory Animals published from Directive 2010/63/EU of the European Parliament and approved by the IRCCS Neuromed Animal Care Review Board and by “Istituto Superiore di Sanità” (permit number: 1163/2015- PR).
Assessment of motor function and disease progression in R6/2 mice
Fine-motor skills and coordination were performed using well-validated motor tests according to the standard recommendations. All tests took place during the light phase of the light–dark cycle. Six mice per experimental group were used in each test. All mice received training for 2 consecutive days on each instrument and task before performing motor behavior measurements. Before training and testing, mice underwent a period of habituation to the testing room and equipment. Motor coordination and balance were tested on the rotarod apparatus as previously described18. Briefly, mice were tested at fixed speed (20 rpm) on the rotarod (Ugo Basile) for 1 min. Each mouse was tested in three consecutive trials of 1 min each, with 1 min rest between trials. The time spent on the rotarod in each of the three trials was averaged to give the overall time for each mouse.
Skilled walking, limb placement and limb coordination were all assessed by the ladder rung walking task as previously described18. All tests were carried out once per week until the 11th week of age. Concomitant with the analysis of motor performance, animal body weight was also measured. All mouse cages were daily examined in order to determine disease progression and the overall wellbeing of mice.
Blood pressure measurements
Blood pressure was measured using the BP-2000 instrument (Visitech systems). The tail cuff method was carried out as described previously19. After four days training period, basal systolic and diastolic blood pressure was daily measured for one week in conscious and unrestrained R6/2 mice at different time points during the symptomatic stage of the disease (7, 10 and 12 week old mice) and in age-matched WT littermates.
Ex vivo vascular reactivity in resistance vessels
Vascular reactivity studies were carried out in second-order branches of the mesenteric arterial tree or in femoral arteries from symptomatic HD mice (12 week old) and age-matched WT littermates.
Briefly, vessels were excissed from mice and adventitial fat was carefully removed under a dissection microscope (Nikon, SMZ645). Arteries were then mounted on pressure myograph (DMT Danish Myosystem) filled with Krebs solution (pH 7.4) maintained at 37 °C as previously described19. After an equilibration period of 60 minutes, vasoconstrictive response was assessed in presence of 80 mM KCl or in presence of increasing doses of phenylephrine (1 × 10−9 to 10−5 M) until a plateau was reached. Vessels were then washed at least three times in order to stabilize the vascular tissue. Endothelium-dependent and -independent relaxations were assessed in phenylephrine pre-constricted vessels by measuring the vasorelaxant response to cumulative concentrations of acetylcholine (1 × 10−9 to 10−5 M) or nitroglycerine (1 × 10−9 to 10−5 M), respectively. Moreover, in order to assess the contribution of NO-signaling to vascular function in our symptomatic HD mice, mesenteric arteries from both WT and R6/2 mice were pre-treated with the direct NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 300 μM, 30 min) before performing the analysis of acetylcholine-induced vasorelaxation.
Ex vivo vascular reactivity in capacitance vessels
To test the vascular response in the capacitance vessels from symptomatic HD mice (12 week old) and age-matched WT littermates, we studied aorta and carotid arteries. In detail, after the excision of vessels from mice, fat tissue was careful removed and vessels cross-sectioned into 2 mm long rings. Two stainless steel wires were inserted into the vascular lumen of aorta, placed in a chamber and connected to a force transducer (WPI). Carotid arteries were mounted in a wire myograph (model 410 A, Danish MyoTechnogy, Aarhus, Denmark) over 25-μm tungsten wires and placed in organ baths filled with aerated Krebs solution connected to a force transducer. After an equilibration period of 60 minutes, vasoconstrictive response was assessed with 80 mM KCl or with increasing doses of phenylephrine (1 × 10−9 to 10−5 M) until a plateau was reached. Vascular response of phenylephrine pre-constricted vessels, to cumulative concentrations of acetylcholine and nitroglycerin was examined to determine the endothelium-dependent and -independent relaxation, respectively.
After isolation, arteries were solubilized in lysis buffer containing 20 mmol/L Tris-HCl, 150 mmol/L NaCl, 20 mmol/L NaF, 2 mmol/L sodium orthovanadate, 1% Nonidet, 100 μg/ml leupeptin, 100 μg/ml aprotinin and 1 mmol/L phenylmethylsulfonyl fluoride. Samples were left on ice for 30 minutes, centrifuged at 10000 g for 15 minutes and supernatants were used to perform Western immunoblot analysis. Total protein levels were determined using the Bradford method. 30 μg proteins were resolved on 7% SDS-PAGE, transferred to a nitrocellulose membrane and immunoblotted with anti-phospho-eNOS S1177 (Cell Signaling, rabbit polyclonal antibody 1:800) and with anti-total-eNOS (Cell Signaling, mouse mAb 1:1000). HRP-conjugated secondary antibodies were used at 1:3000 dilution (Bio-Rad Laboratories). Protein bands were detected by ECL Prime (Amersham Biosciences) and quantitated with Quantity One software (Bio-Rad Laboratories).
All data were expressed mean ± standard error of mean (SEM). Data were statistically analyzed by two-way ANOVA followed by Bonferroni post-hoc analysis, using a dedicated software (GraphPad Prism Software, version 5.0).
Motor performance and disease progression in R6/2 mice
Rigorous evaluation of motor coordination to monitor disease progression and to precisely determine the advanced disease stage in our R6/2 line was performed using standardized procedures as previously described18. As expected, HD mice developed a measurable neurological phenotype by 7–8 weeks of age coherently with the original report16 and, displayed a marked and progressive deterioration in motor performance when compared to WT mice (Fig. 1A,B) as the disease progressed. The severity of these abnormalities worsened gradually until 11 weeks of age, when the advanced disease stage-dependent neurological deterioration classically occurring in this model20, irreversibly affected the overall wellbeing of our mice (Two-way ANOVA. Rotarod: interaction, F (4, 40) = 8.796; p < 0.0001. Horizontal Ladder Task: interaction, F (4, 40) = 8.210; p < 0.0001) (Fig. 1 and Supplementary Tables 1 and 2). As expected, body weight was gradually decreased in R6/2 mice as the disease progressed when compared with age-matched WT littermates (Supplementary Figure 1).
Arterial blood pressure in R6/2 mice
With the aim of delineating a clearer picture of cardiovascular homeostasis in our HD model, measurement of arterial blood pressure was performed using tail-cuff technique in symptomatic R6/2 mice (7,10 and 12 weeks of age) and in age-matched WT controls. Average values of both systolic and diastolic arterial blood pressure, ranging from 85 to 105 mmHg, was not significantly different between the two groups across all the stages of the disease (Two-way ANOVA. SBP; F (6,35) = 0,4336; p = 0.8514; DBP; F(6,35) = 1,443; p = 0.2264) (Fig. 2A,B and Supplementary Figure 2A-B).
Arteries vascular function
In order to fully characterize the functional features of the vasculature in HD, vascular reactivity of both resistance and capacitance arteries from either R6/2 or WT mice at 12 weeks of age was systematically assessed.
Analysis of vasodilator function in phenylephrine pre-contracted femoral (Fig. 3A,B) and mesenteric (Fig. 3C,D) arteries as well as in aortic (Fig. 3E,F) and carotid (Fig. 3G,H) arteries in response to the endothelium-dependent agonist, acetylcholine, and to the endothelium-independent agonist, nitroglycerine, showed no difference between the two genotypes and no signs of vascular dysfunction was detectable in any of the districts analyzed (Two-way ANOVA. Femoral arteries; ACh; F (8,128) = 1,698; p = 0.1048; Nitro; F (8,128) = 0,5216; p = 0.8385; Mesenteric arteries; ACh; F(8,128) = 0,3317; p = 0.9524; Nitro; F(8,128) = 0,3037; p = 0.9634; Aorta; ACh; F (8,128) = 1,379; p = 0.2116; Nitro; F (8,128) = 0,3382; p = 9496. Carotid arteries; ACh; F (8,128) = 0,4320, p = 0.9000; Nitro; F (8,128) = 0,8280; p = 0.5794) (Fig. 3). Consistently with the findings of preserved vasodilator function in advanced disease stage, analysis of vasocostrictor function in response to either potassium or to the α1-adrenergic agonist, phenylephrine, consolidated the evidence of “normal” vascular reactivity in all vessels analyzed (Two-way ANOVA. Femoral arteries; KCl; F (1,5) = 0,5177; p = 0.5040; Phenylephrine; F (8,80) = 0,3364; p = 0.9493. Mesenteric arteries; KCl; F (1,5) = 0,6849; p = 0.4456; Phenylephrine; F (8,80) = 0,3482; p = 0,9440. Aorta; KCl; F (1,5) = 1,777; p = 0,2400; Phenylephrine; F(8,80) = 1,16; p = 0,3335. Carotid arteries; KCl; F (1,5) = 0,2959; p = 6098; Phenylephrine; F (8,80) = 1,589; p = 0.1413) (Fig. 4).
eNOS signaling pathway in advanced stages of HD pathology in R6/2 mice
Nitric oxide is the main determinant of the endothelium-mediated vasorelaxant effects and normally synthesized by the endothelial Nitric Oxide Synthase (eNOS)21,22,23, whose phosphorilation at Ser 1177 residue directly enhances enzyme activity23. Despite the normal vascular function in arteries from symptomatic R6/2 mice, here we investigated whether eNOS pathway was indeed perturbed. Coherently with the evidence of unchanged vasorelaxant responses in advanced stage of HD (Fig. 3), no difference in either eNOS phosphorylation state or protein expression was observed between WT and R6/2 mice (Fig. 5). At functional level, the selective inhibition of NOS by L-NAME blunted acetylcholine-induced vasorelaxation to the same extent in both WT and R6/2 mice and further confirmed the lack of significant difference in the NO-dependent vasorelaxation in both animal groups (Two-way (Two-way ANOVA. F (24,160) =36,88; p < 0,0001) (Fig. 5B).
It is now well established that HD pathology is not only confined to the central nervous system (CNS), but rather comprises a large group of peripheral defects including cardiac phenotype24. In agreement with the evidence that neurological diseases are positively linked to cardiovascular pathology and that CNS abnormalities have a great impact on the pathogenesis of cardiac dysfunction25, it has been postulated that also HD-related cardiac alterations are likely driven by CNS dysfunctions. However, whether there exists any physiopathological interdependence between the progressive disease phenotype and the vascular function in HD has never been assessed before.
While significant cardiac phenotype has been extensively described to typify HD pathology in both human patients26 and animal models27,28, no definitive characterization of vascular function has been exhaustively performed so far. The only comprehensive study that has partially addressed this issue, has shown a defective contractility of some of the systemic arteries only at very advanced age and disease stages of transgenic R6/1 mice14, a HD mouse model with late age at onset, slow disease progression and high life expectancy16. Similar investigation has been recently performed by Kane et al., in a sub-line of R6/2 mouse model which displays a prolonged disease progression and longer lifespan15 than prototypical parent R6/2 mice16,17,29, the most extensively studied and utilized mouse model of HD. Although a derangement in peripheral vascular reactivity has been described, the study of Kane et al., limited the analysis only to the femoral arteries and partially described the vascular phenotype these mice can display15.
Here, with the aim of providing a more detailed functional characterization in HD, an accurate and systematic investigation into vascular reactivity in both central and peripheral arteries was performed in symptomatic parental R6/2 model16,17,29, which differs from that used by Kane et al., in CAG repeat length (160 ± 5 CAG vs 242 ± 1 CAG) and life expectancy (12–13 weeks of age vs. 24 weeks of age)11,16,30.
Analysis of vascular function in our R6/2 line failed to reveal any difference between HD and control mice in all districts analyzed. Moreover, the unaffected vascular reactivity was also accompanied by regular blood pressure profile and unchanged endothelial-mediated NO vasorelaxation. The lack of any alteration in the endothelial function in our symptomatic R6/2 mice was corroborated by unperturbed eNOS signaling in vessels from the same mice. Curiously, our findings did not found full support on the previous study15. The reason for this discrepancy is not clear, however it could be likely attributable, at least for the vasoconstrictor function, to the different genotypes (CAG length) and the dependent and inverted U-shaped profile of disease progression and life expectancy31. On the other hand, although apparently different, endothelial function showed a comparable vasodilator profile in HD mice in both mouse lines at 12 weeks of age. The endothelial dysfunction described by Kane and collaborators in control mice at similar age, and its improvement with aging was rather unexpected.
In this study, the overall absence of vascular dysfunction in our symptomatic R6/2 mice does not exclude that such a specific phenotype may develop later in the disease, that in the case of this mouse line is unlikely to happen because of the characteristic short lifespan and high rate of early death that can occur before peripheral perturbations appear. This hypothesis is also supported by the evidence of impaired vascular function only in aged diseased animal model14. From our perspective, the perfect overlapping profile of vascular reactivity and blood pressure as well as the lack of any change at molecular levels between WT and HD mice definitively bypasses the relative small sample size that could normally represent a limitation.
To our knowledge, this study presents the first full characterization of vascular function in HD transgenic R6/2 mice (160 ± 5 CAG).
Collectively, the combination of functional and biochemical experiments highlights a normal vascular phenotype in these mice and indicates that motor abnormalities do not depend on vascular dysfunction in our mouse model. Therefore, we can conclude that our findings delineate limitations on the usefulness of this mouse line when studying certain aspect associated with aging during the progression of the disease.
How to cite this article: Di Pardo, A. et al. Motor phenotype is not associated with vascular dysfunction in symptomatic Huntington’s disease transgenic R6/2 (160 CAG) mice. Sci. Rep. 7, 42797; doi: 10.1038/srep42797 (2017).
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