Mutations of X-linked gene Methyl CpG binding protein 2 (MECP2) are the major causes of Rett syndrome (RTT), a severe neurodevelopmental disorder. Duplications of MECP2-containing genomic segments lead to severe autistic symptoms in human. MECP2-coding protein methyl-CpG-binding protein 2 (MeCP2) is involved in transcription regulation, microRNA processing and mRNA splicing. However, molecular mechanisms underlying the involvement of MeCP2 in mRNA splicing in neurons remain largely elusive. In this work we found that the majority of MeCP2-associated proteins are involved in mRNA splicing using mass spectrometry analysis with multiple samples from Mecp2-null rat brain, mouse primary neuron and human cell lines. We further showed that Mecp2 knockdown in cultured cortical neurons led to widespread alternations of mRNA alternative splicing. Analysis of ChIP-seq datasets indicated that MeCP2-regulated exons display specific epigenetic signatures, with DNA modification 5-hydroxymethylcytosine (5hmC) and histone modification H3K4me3 are enriched in down-regulated exons, while the H3K36me3 signature is enriched in exons up-regulated in Mecp2-knockdown neurons comparing to un-affected neurons. Functional analysis reveals that genes containing MeCP2-regulated exons are mainly involved in synaptic functions and mRNA splicing. These results suggested that MeCP2 regulated mRNA splicing through interacting with 5hmC and epigenetic changes in histone markers, and provide functional insights of MeCP2-mediated mRNA splicing in the nervous system.
Mutations in the X-linked gene MECP2, which encodes methyl-binding protein 2 (MeCP2), are the major causes of neurodevelopmental disorder Rett syndrome (RTT) occurred mainly in girls1. RTT patients show severe neurological phenotypes such as mental retardation, functional loss in language, autistic features and motor defects2. Several mouse models with Mecp2 depletion have been established and used as powerful tools to explore the relationship between MeCP2 and Rett syndrome, as Mecp2-null mice display phenotypes similar to RTT patients3,4. Previous studies have shown that pathogenesis of RTT mainly attributed to MeCP2 dysfunctions in the nervous system, which highlights the essential role of MeCP2 for neuronal development and functions5,6,7,8. Recently, plenty of studies have investigated the critical roles of MeCP2 in different types of neurons and different cell types of the brain6,9,10,11,12,13; however, the molecular mechanisms for MeCP2 to exert its functions in different cell types remain largely unknown.
MeCP2 was initially identified as a protein showing high affinity to methylated CpG islands14,15 and further studies revealed that it served as a transcriptional repressor by binding to highly methylated genomic regions16. It was also shown that MeCP2 could interact with several transcriptional repressor complexes such as HDAC-mSin3A and NCoR-SMRT complex to repress gene transcription16,17,18. Although it has been confirmed that MeCP2 could repress gene expression in neurons by many studies19, studies in Mecp2-null mice showed that about 70% genes were down-regulated and this may be due to MeCP2 interaction with CREB to stimulate gene expression20. What’s more, it was revealed that MeCP2 could bind to DNA regions containing high level of 5-hydroxymethylcytosine (5 hmC) to promote gene expression21. Therefore MeCP2 was considered as a transcriptional modulator to regulate gene expression bi-directionally. In addition, studies also showed that MeCP2 was involved in the regulation of microRNA expression10,22,23, and MeCP2 could interact with Drosha/DGCR8 complex and other factors to modulate microRNA processing24,25. Subsequent studies demonstrated that MeCP2 could also interact with splicing factors such as Y-box-binding protein 1 (YB-1) to regulate RNA splicing process26,27. And aberrant RNA splicing events were detected in Mecp2308/Y mice that express a truncated MeCP2 protein and recapitulate multiple clinical features of RTT patients26. Recent studies in non-neuronal cell lines IMR90 and HCT116 confirmed the involvement of MeCP2 in regulating alternative splicing, by showing that MeCP2 was enriched in included alternative splicing exons (ASEs) with highly methylated status, and MeCP2 knockdown led to dysregulated alternative splicing in these cell lines28.
Alternative splicing is a critical post-transcriptional process, which produces divergent mRNA isoforms from a single primary transcript to generate proteins with distinct functions or to modulate protein expression levels via the nonsense-mediated mRNA decay pathway (NMD)29,30. High-throughput studies showed that alternative splicing process was particularly more common and conserved in the central nervous system as compared to other tissues, and the precise regulation of alternative splicing was critical for divergent aspects of neuronal development via modulation of protein diversity and abundance31,32,33,34,35.
It was well-established that splicing factors were mainly RNA-binding proteins which regulated alternative splicing at RNA level36. Mutations in RNA splicing factors and aberrant alternative splicing have been implicated in various neurological diseases such as amyotrophic lateral sclerosis (ALS), autistic spectrum disorders (ASD) and Rett syndrome26,35,37,38,39,40. Recently, accumulated evidences indicated that chromatin structures and epigenetic modifications such as DNA methylation, hydroxymethylation and histone modifications were also highly involved in mRNA splicing41,42,43,44,45,46. However, it is still unclear how modifications at DNA and chromatin level lead to splicing changes at mRNA level.
In previous studies, MeCP2 was linked to alternative splicing by both interacting with splicing factors and binding to alternative splicing exons with high methylated status. Here we examined the MeCP2-interacting proteins in 293 T cells, cultured mouse cortical neurons, cortical tissues of Mecp2 null and wild type rat by mass spectrometry and found that the majority were proteins involved in RNA splicing. Furthermore, we analyzed the impact of Mecp2 knockdown on alternative splicing processes in cultured mouse cortical neurons by RNA sequencing (RNA-Seq) analysis and identified widespread alternative splicing changes upon Mecp2 deletion. Protein binding status such as MeCP2 binding and RNA polymerase II (Pol II) binding sites, and epigenetic signatures such as 5-methylcytosine (5 mC), 5 hmC and histone modifications in these MeCP2-regulated exons were examined through ChIP-Seq data analysis. It was shown that MeCP2 binding was significantly correlated with Pol II distribution and epigenetic markers including 5 hmC, H3K4me3 and H3K36me3 display specific signatures in MeCP2-regulated exons. Furthermore, functional analysis of genes containing these MeCP2-regulated exons showed that MeCP2-mediated alternative splicing are important for neuronal functions such as synaptic organization, intracellular transport and gene expression regulation and RNA processing. Taken together, These results suggested that in the central nervous system, MeCP2 may serve as a scaffold protein for epigenetic modifications and splicing factors to regulate alternative splicing, and such regulation was critical for normal neuronal functions.
MeCP2-associated proteins mainly involved in RNA splicing process
Mecp2-null rat was generated using TALEN-based gene targeting technology (Fig. 1a). Mecp2 disruption with 10 bp deletion was confirmed by PCR and sanger sequencing (Fig. 1b). Furthermore, depletion of MeCP2 protein was confirmed by Western blot using protein lysates collected from Mecp2-null (KO) and wild-type (WT) littermate rats (Fig. 1c). To identify MeCP2-associated proteins in vivo, we took advantage of the tandem Histidine residues within the MeCP2 protein (a.a. 366–372), suggesting that Ni-NTA resin could be directly used for purifying MeCP2 and its associated proteins from cortical lysates of Mecp2-null (KO) and wild-type (WT) littermate rats. We thus performed immunoprecipitation using Ni-NTA resin in protein lysates from Mecp2-null and WT rat cortex. Candidate MeCP2-associated proteins found in WT, but not KO lysates, were identified with mass spectrometry analysis (Fig. 1d,e).
To identify MeCP2-binding proteins more accurately, we further performed Ni-NTA purification in 293 T cells expressing His-MeCP2 to identify proteins showing tightly binding affinity to MeCP2. Moreover, we performed immunoprecipitation with anti-MeCP2 antibody in mouse cortical neurons in order to purify endogenous MeCP2-associated proteins in neurons (Fig. 1d). Overall, we identified 131 proteins in 293 T cells while 488 proteins in mouse cortical neurons and 406 proteins in rat cortex, showing high or low affinities to MeCP2 (Fig. 1e). We then performed GO analysis for proteins identified in either mouse cortical neurons or rat cortex, and found that these proteins were mainly enriched in posttranscriptional regulation such as RNA splicing and mRNA processing (Fig. 1f). We further performed protein-protein interaction network analysis for these 58 MeCP2-binding proteins identified in both mouse cortical neurons and rat cortex and revealed that these proteins were tightly connected with splicing factors as the core node (Fig. 1g). Overlap analysis among MeCP2-associated proteins identified in these three experiments showed that 37 MeCP2-binding proteins identified in 293 T cells (excluding Keratin and ribosomal proteins) were also observed in either mouse cortical neurons or in rat cortex and were considered as high-confidence MeCP2-binding proteins. In consistent with above GO analysis, 17/37 high-confidence proteins were splicing factors, indicating that MeCP2 was an important regulator of RNA splicing. Then protein-protein interaction network analysis was performed for these 37 proteins and it was shown that these proteins were closely associated, suggesting that their functions were tightly connected (Fig. 1h).
Widespread exon usage changes in neurons with MeCP2 knockdown
Mass spectra and protein interaction studies revealed that MeCP2-binding proteins were primarily enriched in RNA splicing/processing functions, so we examined the impact of Mecp2 knockdown on alternative splicing process in mouse cortical neurons by RNA-seq. Mouse cortical neurons were cultured in vitro and infected at DIV2 with lentivirus expressing shRNA targeting either mouse Mecp2 or scrambled sequence. Mecp2 knockdown efficiency in these neurons was confirmed by both real-time PCR and western blot (Supplementary Fig. 1a,b). RNA deep sequencing was then performed and DEXseq software package was used to analyze exon usage differences between Mecp2-knockdown neurons and control neurons. Our analysis identified 1225 exons up-regulated in Mecp2-knockdown neurons and 608 exons down-regulated in Mecp2-knockdown neurons (Padjust < 0.05) (Fig. 2a). We further analyzed transcriptional levels of genes contained down-regulated or up-regulated exons. It was shown that the expression of 231/482 genes containing down-regulated exons was deregulated (103 were downregulated and 128 were upregulated) while the expression of 477/912 genes containing up-regulated exons was deregulated (185 were downregulated and 292 were upregulated) in Mecp2-knockdown neurons. We then selected 8 identified exons whose expression was either increased or decreased in Mecp2-knockdown neurons for real-time PCR verification. And our real-time PCR results confirmed the reliability of DEXseq analysis (Fig. 2b). In addition to RNA splicing analysis in Mecp2-knockdown neurons, we further analyzed RNA splicing changes in Mecp2-null rat hippocampus and observed 1130 exon usage changes as compared to wild-type rat hippocampus (P < 0.01, Supplementary Table 1). In addition to exon usage analysis, we further performed RNA splicing analysis using software ASD described previously47 to classify different modes of alternative splicing events. Currently eight modes of alternative splicing events were defined including cassette exon, alternative 5′ splice site, alternative 3′ splice site, retained intron, alternative first exon, alternative last exon, multiple cassette exons and mutually exclusive exon (Fig. 2c). With ASD software, a total of 45112 splicing events were identified and 536 events showed significantly changes in mouse Mecp2-knockdown neurons (Supplementary Table 2, Fig. 2d) while in Mecp2-null rat hippocampus, a total number of 22179 alternative splicing events were detected and 2637 events changed significantly as compared to wild-type rat hippocampus (Supplementary Table 3, Fig. 2e).
We further compared the exons identified by DEXseq with exons identified by ASD software. It was shown that 32 differentially expressed exons revealed by both softwares and at least 17 exons were located in genes critical for neuronal development and synaptic functions (Fig. S1c).
Distribution of MeCP2 and Pol II proteins in MeCP2-regulated exons
We further examined the distribution of MeCP2 and RNA polymerase II (Pol II) proteins in MeCP2-regulated exons identified in Mecp2-knockdown neurons. ChIP-seq data for MeCP2 and Pol II binding status in neurons under similar conditions was selected and re-analyzed for up-regulated, down-regulated and unchanged exons of MeCP2-knockdown neurons respectively. Our analysis revealed that both MeCP2 and Pol II were highly enriched in down-regulated exons (Fig. 3a,b, Supplementary Fig. 2a,b), indicating that MeCP2 and Pol II were critical for exon inclusion in normal conditions. Intriguingly, though the distribution of Pol II protein in up-regulated exons was similar to unchanged exons, the distribution of MeCP2 was significantly higher in splice acceptor site of up-regulated exons as compared to unchanged exons (Fig. 3a,b, Supplementary Fig. 2b), which suggested that MeCP2 may modulate exon exclusion in normal conditions via mechanisms independent of Pol II-mediated transcriptional elongation.
Distribution of epigenetic markers in MeCP2-regulated exons
MeCP2 was a methyl/hydroxymethyl-DNA binding protein and closely associated with nucleosome. As DNA methylation status and histone modifications were involved in alternative splicing process, we further examined whether epigenetic markers such as 5 mC, 5 hmC and histone methylation such as H3K4me3 and H3K36me3 distributed differentially in MeCP2-regulated exons. ChIP-seq data for 5 mC, 5 hmC, H3K4me3 and H3K36me3 in similar conditions as ours was described previously and chosen to determine their distribution in up-regulated, down-regulated and unchanged exons of MeCP2-knockdown neurons. It was shown that DNA methylation level in either down-regulated or up-regulated exons were lower than that in unchanged exons (Fig. 3c). However, DNA hydroxymethylation level was significantly higher in down-regulated exons as compared to up-regulated or unchanged exons (Fig. 3d). These results indicated that MeCP2 binding to down-regulated exons in neurons might be dependent on DNA hydroxymethylation but not DNA methylation.
In addition to DNA methylation status, distribution of histone modifications such as H3K4me3 and H3K36me3 were also examined and it was shown that H3K4me3 was highly enriched in down-regulated exons while H3K36me3 was enriched in up-regulated exons (Fig. 3e,f). It has been reported previously that H3K4me3 was involved in exon inclusion while H3K36me3 was involved in exon exclusion48, so our results suggested that MeCP2 might be critical for the H3K4me3- and H3K36me3-mediated alternative splicing.
Functional analysis of genes containing MeCP2-regulated exons
It has been reported that loss-of-function of MeCP2 in cultured neurons or mouse models impaired neuronal functions in different aspects including dendritic growth, axon transport and synaptic functions6,49,50. Here we further performed functional enrichment analysis to investigate the functional importance of genes containing MeCP2-regulated exons in neurons. It was shown that genes containing MeCP2-regulated exons were highly enriched in axon cargo transport and synaptic organization (Fig. 4a,b). In addition, GO analysis was also performed for genes containing MeCP2-regulated exons identified in rat cortex and revealed that axon cargo transport was also the highly enriched GO terms (Fig. S3a). We further compared genes containing MeCP2-regulated exons to curated database of genes encoding synaptic proteins or candidate gene lists of autism spectrum disorders. Significant overlap was observed with genes encoding synaptic proteins including synaptome, postsynaptic proteome, presynaptic proteome and several complexes such as NMDAR and mGluR5 (Supplementary Table 4). Overlapping with candidate gene sets of autism spectrum disorders (SFARI Autism database) was also significant (>10%), in consistent with the implication of MeCP2 in autism spectrum disorders (Supplementary Table 4). In addition, significant functional enrichment was also observed in intracellular transport, RNA processing, chromatin organization (Fig. 4b). These results suggested that MeCP2-mediated alternative splicing was important for normal neuronal functions and likely involved in epigenetic or post-transcriptionally regulation.
Exons were further annotated according to protein-coding ability of related transcripts and divided into coding exons which was in the transcripts of protein-coding mRNAs or non-coding exons only in non-coding transcripts including non-mediated decay or processed transcripts. It was speculated that changes of coding exons usage may influence protein diversity while non-coding exons might be involved in the regulation of translation and protein expression, so MeCP2-regulated exons were further classified into these two classes for functional analysis. It was shown that exons down-regulated in MeCP2-knockdown neurons were mainly coding exons (559/608) while a large fraction of non-coding exons were up-regulated (413/462) (Fig. 4c). GO functional analysis showed that non-coding exons were mainly enriched in functions associated with chromatin and RNA regulation such as RNA processing, chromatin modification and chromatin organization; while coding exons were enriched in neuronal physiological functions such as intracellular transport and synapse organization (Fig. 4d). These results indicated that MeCP2-mediated RNA splicing had divergent functional consequences, further confirming the multifaceted functions of MeCP2.
Several studies confirmed the involvement of MeCP2 in regulating alternative splicing and provided insights into the underlying mechanisms, by showing that MeCP2 may modulate alternative splicing through its interaction with both splicing factors and epigenetic markers such as 5 mC26,28,51. However, comprehensive analysis for the role of MeCP2 in alternative splicing in neurons remained elusive. Here we explored the potential mechanisms underlying MeCP2-mediated alternative splicing at protein, RNA and DNA level in neurons using mass spectrum, RNA-Seq and ChIP-Seq data re-analysis. Our results showed that MeCP2 depletion in cultured cortical neurons led to significant splicing changes by RNA-Seq, confirming the critical role of MeCP2 in alternative splicing regulation. Mass spectrum for MeCP2-binding proteins showed that MeCP2 could interact with transcriptional regulators, splicing factors and chromatin-related modifiers (Supplementary Fig. 3), which suggested that MeCP2 might serve as a bridge for chromatin-related modifiers and splicing factors to modulate alternative splicing. Moreover, ChIP-Seq data re-analysis for MeCP2, 5 mC and 5 hmC revealed that MeCP2 distribution in down-regulated exons of Mecp2-knockdown neurons was positively correlated with 5 hmC but not 5 mC, which is not in consistent with studies in non-neuronal cell lines IMR90 and HCT11628. This inconsistency might reflect that MeCP2 functions varied depending on cell types, and is consistent with previous studies showing that the role of 5 hmC in alternative splicing regulation was tissue specific46. We further analyzed ChIP-Seq data for Pol II and confirmed that MeCP2-mediated alternative splicing might be coupled to Pol II-regulated transcriptional elongation28. Pol II distribution analysis in this study also confirmed the reliability of our ChIP-data re-analysis. We then further examined the ChIP-Seq data for H3K4me3 and H3K36me3, which has been implicated in the regulation of exon inclusion and exon skipping respectively, and revealed that H3K4me3 was highly enriched in down-regulated exons while H3K36me3 was enriched in up-regulated exons of Mecp2-knockdown neurons. As MeCP2 was enriched in down-regulated exons and MeCP2 could interact with CHD1 and PARP1 (Supplementary Fig. 3), which were H3K4me3-related proteins, it is possible that MeCP2 might be involved in H3K4me3-mediated exon inclusion. Taken together, MeCP2 might serve as a scaffold protein for alternative splicing, by “translating” epigenetic modifications into signals to guide splicing factors for splicing regulation.
In addition to investigations about mechanisms underlying MeCP2-mediated alternative splicing, we also examined the functional enrichment of genes containing MeCP2-regulated exons in cultured cortical neurons. It was shown that MeCP2-mediated alternative splicing was highly enriched in two major functional classes: one is associated with neuronal physiological functions such as intracellular transport, axon cargo transport and synaptic organization while another is closely related to chromatin and RNA regulation such as chromatin structure, RNA processing and splicing. We noticed that according to the protein-coding ability, exons could be further divided into coding exons and non-coding exons, and functional enrichment reanalysis for coding and non-coding exons regulated by MeCP2 showed that coding exons mainly enriched in neuronal physiological functions while non-coding exons mainly enriched in chromatin and RNA regulatory functions.
Moreover, we further found that most of the MeCP2-regulated non-coding exons were up-regulated in MeCP2-knockdown neurons (413/462). As non-coding exons might inhibit gene expression via protein translational regulation, it is possible that in normal neurons, MeCP2 inhibited the inclusion of non-coding exons to promote gene expression while Mecp2 knockdown led to repression of gene expression at protein level. Our analysis thus indicated that MeCP2-mediated alternative splicing might influence neuronal functions via two different strategies: one is to regulate protein diversity by coding exons, and another is to regulate protein expression by non-coding exons. Our studies not only systematically explore the mechanisms underlying MeCP2-mediated alternative splicing, but also provide insights into the roles of MeCP2-mediated alternative splicing, which could influence both protein diversity and protein expression level in neurons.
Materials and Methods
Ethical approval and informed consent
The experimental protocol and procedures followed the guidelines and regulations issued by SIBS, CAS and were approved by Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.
Mecp2 knockdown in neurons
The shRNA specifically against mouse Mecp2 and related lentivirus preparation has been described previously24. Briefly, embryonic day 15–16 mouse cortical cells were isolated and cultured in 6-well plates. Lentivirus expressing either shRNA against mouse Mecp2 or control shRNA was added at day in vitro (DIV) 2, and neurons were cultured for another 3–5 days. Total RNAs/proteins were collected for further analysis.
Generation of Mecp2-null rat
Mecp2-null rat was generated by transcription activator-like effector nucleases (TALENs)-mediated gene targeting. In brief, TALENs mRNAs specifically targeting against exon2 of the rat Mecp2 gene were designed, tested and prepared by the Beijing Biocytogen Co. Ltd. Rat zygotes were injected with Mecp2 TALENs mRNAs and were then implanted into surrogate rat mothers. Mecp2 locus was determined in F0 rats by PCR and sanger sequencing and rats carrying disrupted Mecp2 genome locus were chosen for F1 preparation. MeCP2 expression in F1 rats was determined by western blot. Mecp2-null rats and wild-type (WT) littermates were used for subsequent experiments.
Purification of MeCP2 protein complexes
MeCP2 protein complexes were purified as described previously52. Briefly, His-MeCP2 expression plasmid was transfected into 293 T cells and purified by Ni-NTA His binding resin (Millipore, Bedford, MA, USA) was used to purify overexpressed His-MeCP2 in 293-T cells, and after washing extensively for four times with Urea buffer, mass spectrometry was performed to identify the proteins closely associated with MeCP2.
Immunoprecipitation in cultured mouse cortical neurons and in rat cortex
As described previously52, cultured mouse cortical neurons were collected and lysed in RIPA buffer and MeCP2 antibody (Cell signaling technology, Beverly, MA, USA) was used for protein immunoprecipitation and normal rabbit IgG (Santa Cruz, CA, USA) was used as negative control.
Immunoprecipitation in rat cortex was performed as following: Mecp2-null rat cortex and WT littermate cortex were collected and lysed with RIPA buffer, and were purified by Ni-NTA His binding resin (Millipore, Bedford, MA, USA) due to the tandem Histidine residues in the MeCP2 protein.
The purified protein was suspended and reduced in 50 μl lysis buffer (7 M urea, 2 M thiourea, 10 mM HEPES, 1 mM sodium orthoranadate, 5 mM sodium fluoride, 5 mM β-glycerophosphate, 10 mM DTT, pH 8.0, then alkylated with 50 mM iodoacetamide. After reducing urea concentration to 2 M, the samples were digested with trypsin (Promega, USA) at 37 °C (1:100 w/w) overnight. The digested peptide mixtures were acidified with trifluoroacetic acid (TFA) at 1% final concentration and desalted by reversed-phase C18 Sep-Pak cartridge (Millipore, USA).
Mass spectrometry analysis
Peptides were loaded and separated on an EASY-nLC1000 LC (Thermo Scientific) coupled on line to the Q-Exactive Orbitrap mass spectrometer (Thermo Scientific). The microcapillary trap column was constructed in a 25 mm × 75 silica capillary, packed with 5-μm Luna C18 stationary phase (Phenomenex). The analytical column was constructed in a 150 mm × 75 μm silica capillary tip pulled with a column puller and packed with 3-μm Luna C18 stationary phase. The organic gradient was over 120 min using buffers A and B. Buffer A contained 2% acetonitrile with 0.1% acetic acid. Buffer B contained 98% acetonitrile with 0.1% acetic acid. The separation was performed in 90 min at a flow rate of 300 nl/min, with a gradient of 2% to 6% buffer B in 1 min, followed by 6% to 24% in 61 min, 24% to 100% in 14 min, then 100% buffer B for 5 min and 100% to 0% in 1 min and finally 0% buffer B in 8 min. Full MS were acquired at a resolution of 70,000 with an AGC target value of 3 × 106 and a maximum injection time of 100 ms. Target value for the MS/MS scans was 1 × 105 charges with a resolution of 17,500 and a maximum injection time of 50 ms. The raw files were analyzed by MaxQuant software (version 220.127.116.11) against the latest release of the human, mouse or rat Uniprot database respectively (http://www.uniprot.org/proteomes/).
Hippocampal tissue from Mecp2-null rats or wild-type rats at postnatal 30 days and neurons infected with lentivirus expressing either shRNA against mouse Mecp2 or control shRNA were prepared in biological triplicate and total RNA was isolated by TRIzol reagent (Invitrogen). Then RNase-free DNaseI was used to remove potential genomic DNA contamination and polyA + mRNA was isolated using Dynabeads oligo(dT) for cDNA preparation. Superscript II reverse transcriptase (Invitrogen) and random hexamer primers were used for double-stranded cDNA synthesis. Then mRNA-seq libraries for mouse neurons were established according to the standard Illumina protocol. RNA-seq was performed using Illumina Hiseq 2000 > 45 million 2 × 100 reads per sample was produced and alignment was performed using mouse genome database GRCm38 version 67. Raw data for RNA-seq was submitted to NCBI GEO database #79993. Complementary DNA (cDNA) libraries of rat hippocampus for single-end sequencing were prepared using Ion Total RNA-Seq Kit v2.0 (Life Technologies) according to the manufacturer’s instructions. The cDNA libraries were then processed for the Proton Sequencing process according to the commercially available protocols. Samples were diluted and mixed, the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) for preparing the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles of samples was loaded on to 1 P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers according to Ion PI Sequencing 200 Kit v2.0 (Life Technologies).
Quantitative Real-Time PCR
Quantitative real-time PCR assay was performed as described previously. Briefly, total RNA was isolated by TRIzol reagent (Invitrogen) and then converted to cDNA using 5XPrimeScript RT Master Mix (TAKARA). SYBR Premix from Toyobo was used with Rotor-Gene Q machine (QIANGEN). Data was analyzed with comparative CT method. Primers used in this study were as follows:
Gria1-005 for: ACATTGAGCAACGCAAGC;
Gria1-005 rev: CCCTGCTCGTTCAGTTTTAAC;
Gria1 for (internal control): GTCCGCCCTGAGAAATCCAG;
Gria1 rev (internal control): CTCGCCCTTGTCGTACCAC;
Nrxn1 for (internal control): CGGAGACCCCAGTCCTATGG;
Nrxn1 rev (internal control): AACATCATCGGATGCAAATGGA;
Nrxn1-024 for: AGGCATTGGACACGCTATG;
Nrxn1-024 rev: TTACTGACTGGTGACCCTGG;
Nrxn1-027 for: TGCCAAAACTGGTCCATGC;
Nrxn1-027 rev: GCAAGTCAGCTTTCATCAATGC;
Gabrg2-027 for: CTTTGGTGGAGTATGGCAC;
Gabrg2-027 rev: AAGGAAAACATCCGAAGAAGAG;
Gabrg2 for (internal control): CAGACTTACATTCCCTGCAC;
Gabrg2 rev (internal control): CTTGGGCAGAGATTTTCTGG;
Camta1 for (internal control): CTATGTCCATTCCTCCATCATAC;
Camta1 rev (internal control): TAGCCCACTCCTTCTTGTC;
Camta1-013 for: CCGAAAAAGCTGCTTGAATGTC;
Camta1-013 rev: AAGGTAATGCAGGTCAGGGG;
Camta1-001 for: AGCGTTTCCCAAAGTGTATTC;
Camta1-001 rev: ACTTGAACATTTCGGCAGAC;
Rcan1-201 for: ACAATTTTAGCTCCCTGATTGC;
Rcan1-201 rev: GCTCTTAAAATACTGGAAGGTGG;
Rcan1 for (internal control): AAACTTCAGCAACCCCTTATC;
Rcan1 rev (internal control): CAGGTGTGAACTTCCTATGTG;
Top3b-intron for: GAAAGGAACACCGTACAGC;
Top3b-intron rev: TCACAACTAGGAATAGGATAGGG;
Top3b for (internal control): GTGCTCATGGTAGCAGAAAAG;
Top3b rev (internal control): GCAAAGGTTCCTGTGTACTTG.
Exon usage analysis
Alternative spliced exons RNA-Seq reads were aligned to the mouse genome by the company, and bam files were obtained. We used the DEXSeq package53 in Bioconductor to identify significantly differentially regulated exons in MeCP2 knockdown neurons based on these bam files, according to Version 67 of the ensemble annotation GTF file for mouse. Exons with a p-value less than or equal to 0.05 after multi-test correction were considered significantly differentially regulated. Significantly downregulated exons were considered as “inclusive” exons, while significantly up-regulated exons were “exclusive” exons.
Alternative Splicing Detection
We selected the Alternative Splicing Detector (ASD, available on http://www.novelbio.com/asd/ASD.html) as the tool to detect the differentially alternative splicing cases based on the bam file after mapping according to the P-Value threshold (P-Value < 0.05)47.
ChIP-Seq data analysis
ChIP-Seq data in BigWig format for MeCP2 binding sites in cultured embryonic mouse cortical neurons were downloaded from the GEO database (GSE3185154). ChIP-Seq data in BigWig format for PolII binding and histone markers in embryonic mouse cortical neurons were downloaded from the GEO database (GSE2116155). DNA methylation data (5mc and 5hmc) and ChIP-Seq data for histone markers in BigWig format in embryonic mouse cortex neurons were downloaded from the GEO database (GSE3811856). The BigWig files were then transformed to bedgraph format, and narrow peaks were called by using MACS57. The generated peak regions for PolII, histone markers and DNA methylation were then mapped to the regions of the exclusive exons and the inclusive exons. Each exon region was extended as the length of the exon region to both the upstream and the downstream of the exon, and were also mapped with the generated peak regions. Results for all the exclusive exons and all the inclusive exons were combined together to draw figured to display the epigenetic dynamics in and around these exons. A sliding window of 10 bp was used to draw each point. The same was applied to all exons used in this analysis as the background. KS test and T test were used to examine whether there was a significant different among the distributions of each histone marker/Methylation type/Pol II binding in exclusive exons, inclusive exons and all background exons used in this analysis.
Functional enrichment analysis
Genes containing exclusive exons and inclusive exons were obtained. Functional enrichment analysis was applied to these genes by using the online server DAVID (https://david.ncifcrf.gov).
Exons were annotated according to ensemble annotation file using DEXSeq. Then these exons were compared with protein-coding exons annotated in ensemble database. Those showing no overlap with protein-coding exons were classified as non-coding exons.
Comparison between genes containing MeCP2-regulated exons and functional gene list
The overlap of genes containing MeCP2-regulated exons with different functional gene lists was analyzed as described previously58. In brief, gene lists of postsynaptic proteome, NMDAR complex, mGluR5 complex, AMPAR complex and synaptome were downloaded from http://www.genes2cognition.org/, the pre-synaptic proteome has been described in Croning et al., Table S459. Gene list for autism spectrum disorders was from Simons SFARI Gene database60. Statistical analysis for overlap between genes containing MeCP2-regulated exons and different gene lists was determined by Fisher’s exact test.
How to cite this article: Cheng, T.-L. et al. Regulation of mRNA splicing by MeCP2 via epigenetic modifications in the brain. Sci. Rep. 7, 42790; doi: 10.1038/srep42790 (2017).
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Amir, R. E. et al. Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet 23, 185–188, doi: 10.1038/13810 (1999).
Chahrour, M. & Zoghbi, H. Y. The story of Rett syndrome: From clinic to neurobiology. Neuron 56, 422–437, doi: 10.1016/j.neuron.2007.10.001 (2007).
Chen, R. Z., Akbarian, S., Tudor, M. & Jaenisch, R. Deficiency of methyl-CpG binding protein-2 in CNS neurons results in a Rett-like phenotype in mice. Nat Genet 27, 327–331, doi: 10.1038/85906 (2001).
Guy, J., Hendrich, B., Holmes, M., Martin, J. E. & Bird, A. A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome. Nat Genet 27, 322–326, doi: 10.1038/85899 (2001).
Gemelli, T. et al. Postnatal loss of methyl-CpG binding protein 2 in the forebrain is sufficient to mediate behavioral aspects of Rett syndrome in mice. Biol Psychiatry 59, 468–476, doi: S0006-3223(05)00914-510.1016/j.biopsych.2005.07.025 (2006).
Chao, H. T., Zoghbi, H. Y. & Rosenmund, C. MeCP2 controls excitatory synaptic strength by regulating glutamatergic synapse number. Neuron 56, 58–65, doi: 10.1016/j.neuron.2007.08.018 (2007).
Guy, J., Gan, J., Selfridge, J., Cobb, S. & Bird, A. Reversal of neurological defects in a mouse model of Rett syndrome. Science 315, 1143–1147, doi: 10.1126/science.1138389 (2007).
Guy, J., Cheval, H., Selfridge, J. & Bird, A. The role of MeCP2 in the brain. Annu Rev Cell Dev Biol 27, 631–652, doi: 10.1146/annurev-cellbio-092910-154121 (2011).
Maezawa, I., Swanberg, S., Harvey, D., LaSalle, J. M. & Jin, L. W. Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions. J Neurosci 29, 5051–5061, doi: 10.1523/JNEUROSCI.0324-09.2009 (2009).
Szulwach, K. E. et al. Cross talk between microRNA and epigenetic regulation in adult neurogenesis. J Cell Biol 189, 127–U181, doi: 10.1083/jcb.200908151 (2010).
Chao, H. T. et al. Dysfunction in GABA signalling mediates autism-like stereotypies and Rett syndrome phenotypes. Nature 468, 263–269, doi: 10.1038/nature09582 (2010).
Okabe, Y. et al. Alterations of gene expression and glutamate clearance in astrocytes derived from an MeCP2-null mouse model of Rett syndrome. PLoS One 7, e35354, doi: 10.1371/journal.pone.0035354 (2012).
Derecki, N. C. et al. Wild-type microglia arrest pathology in a mouse model of Rett syndrome. Nature 484, 105–109, doi: 10.1038/nature10907 (2012).
Lewis, J. D. et al. Purification, Sequence, and Cellular-Localization of a Novel Chromosomal Protein That Binds to Methylated DNA. Cell 69, 905–914 (1992).
Nan, X. S., Meehan, R. R. & Bird, A. Dissection of the Methyl-Cpg Binding Domain from the Chromosomal Protein Mecp2. Nucleic Acids Research 21, 4886–4892 (1993).
Nan, X. S. et al. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 393, 386–389 (1998).
Lyst, M. J. et al. Rett syndrome mutations abolish the interaction of MeCP2 with the NCoR/SMRT co-repressor. Nature neuroscience 16, 898–902, doi: 10.1038/nn.3434 (2013).
Ebert, D. H. et al. Activity-dependent phosphorylation of MeCP2 threonine 308 regulates interaction with NCoR. Nature 499, 341–345, doi: 10.1038/nature12348 (2013).
Chen, W. G. et al. Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. Science 302, 885–889, doi: 10.1126/science.1086446302/5646/885 (2003).
Chahrour, M. et al. MeCP2, a key contributor to neurological disease, activates and represses transcription. Science 320, 1224–1229, doi: 10.1126/science.1153252 (2008).
Mellen, M., Ayata, P., Dewell, S., Kriaucionis, S. & Heintz, N. MeCP2 binds to 5 hmC enriched within active genes and accessible chromatin in the nervous system. Cell 151, 1417–1430, doi: 10.1016/j.cell.2012.11.022 (2012).
Urdinguio, R. G. et al. Disrupted microRNA expression caused by Mecp2 loss in a mouse model of Rett syndrome. Epigenetics-Us 5, 656–663, doi: 10.4161/epi.5.7.13055 (2010).
Wu, H. et al. Genome-wide analysis reveals methyl-CpG-binding protein 2-dependent regulation of microRNAs in a mouse model of Rett syndrome. Proc Natl Acad Sci USA 107, 18161–18166, doi: 1005595 10710.1073/pnas.1005595107 (2010).
Cheng, T. L. et al. MeCP2 Suppresses Nuclear MicroRNA Processing and Dendritic Growth by Regulating the DGCR8/Drosha Complex. Dev Cell 28, 547–560, doi: 10.1016/j.devcel.2014.01.032 (2014).
Tsujimura, K. et al. miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes. Cell Rep 12, 1887–1901, doi: 10.1016/j.celrep.2015.08.028 (2015).
Young, J. I. et al. Regulation of RNA splicing by the methylation-dependent transcriptional repressor methyl-CpG binding protein 2. Proc Natl Acad Sci USA 102, 17551–17558, doi: 0507856102 10.1073/pnas.0507856102 (2005).
Maxwell, S. S., Pelka, G. J., Tam, P. P. & El-Osta, A. Chromatin context and ncRNA highlight targets of MeCP2 in brain. RNA biology 10, 1741–1757, doi: 10.4161/rna.26921 (2013).
Maunakea, A. K., Chepelev, I., Cui, K. & Zhao, K. Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition. Cell research 23, 1256–1269, doi: 10.1038/cr.2013.110 (2013).
Kornblihtt, A. R. et al. Alternative splicing: a pivotal step between eukaryotic transcription and translation. Nat Rev Mol Cell Bio 14, 153–165 (2013).
Barash, Y. et al. Deciphering the splicing code. Nature 465, 53–59, doi: 10.1038/nature09000 (2010).
Li, Q., Lee, J. A. & Black, D. L. Neuronal regulation of alternative pre-mRNA splicing. Nature reviews. Neuroscience 8, 819–831, doi: 10.1038/nrn2237 (2007).
Zheng, S. & Black, D. L. Alternative pre-mRNA splicing in neurons: growing up and extending its reach. Trends Genet 29, 442–448, doi: 10.1016/j.tig.2013.04.003 (2013).
Fagnani, M. et al. Functional coordination of alternative splicing in the mammalian central nervous system. Genome biology 8, R108, doi: 10.1186/gb-2007-8-6-r108 (2007).
Yap, K. & Makeyev, E. V. Regulation of gene expression in mammalian nervous system through alternative pre-mRNA splicing coupled with RNA quality control mechanisms. Molecular and cellular neurosciences 56, 420–428, doi: 10.1016/j.mcn.2013.01.003 (2013).
Raj, B. & Blencowe, B. J. Alternative Splicing in the Mammalian Nervous System: Recent Insights into Mechanisms and Functional Roles. Neuron 87, 14–27, doi: 10.1016/j.neuron.2015.05.004 (2015).
Matlin, A. J., Clark, F. & Smith, C. W. Understanding alternative splicing: towards a cellular code. Nat Rev Mol Cell Biol 6, 386–398, doi: 10.1038/nrm1645 (2005).
Irimia, M. et al. A Highly Conserved Program of Neuronal Microexons Is Misregulated in Autistic Brains. Cell 159, 1511–1523, doi: 10.1016/j.cell.2014.11.035 (2014).
Xiong, H. Y. et al. The human splicing code reveals new insights into the genetic determinants of disease. Science 347, 144−+, doi: 10.1126/science.1254806 (2015).
Ling, J. P., Pletnikova, O., Troncoso, J. C. & Wong, P. C. TDP-43 repression of nonconserved cryptic exons is compromised in ALS-FTD. Science 349, 650–655, doi: 10.1126/science.aab0983 (2015).
Weyn-Vanhentenryck, S. M. et al. HITS-CLIP and Integrative Modeling Define the Rbfox Splicing-Regulatory Network Linked to Brain Development and Autism. Cell Rep 6, 1139–1152, doi: 10.1016/j.celrep.2014.02.005 (2014).
Luco, R. F., Allo, M., Schor, I. E., Kornblihtt, A. R. & Misteli, T. Epigenetics in alternative pre-mRNA splicing. Cell 144, 16–26, doi: 10.1016/j.cell.2010.11.056 (2011).
Lev Maor, G., Yearim, A. & Ast, G. The alternative role of DNA methylation in splicing regulation. Trends Genet 31, 274–280, doi: 10.1016/j.tig.2015.03.002 (2015).
Chen, M. & Manley, J. L. Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Nat Rev Mol Cell Biol 10, 741–754, doi: 10.1038/nrm2777 (2009).
Zhou, H. L., Luo, G., Wise, J. A. & Lou, H. Regulation of alternative splicing by local histone modifications: potential roles for RNA-guided mechanisms. Nucleic Acids Res 42, 701–713, doi: 10.1093/nar/gkt875 (2014).
Lee, Y. & Rio, D. C. Mechanisms and Regulation of Alternative Pre-mRNA Splicing. Annual review of biochemistry 84, 291–323, doi: 10.1146/annurev-biochem-060614-034316 (2015).
Khare, T. et al. 5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary. Nat Struct Mol Biol 19, 1037–1043, doi: 10.1038/nsmb.2372 (2012).
Zhou, X. et al. Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation. Nucleic Acids Res 42, 4019–4030, doi: 10.1093/nar/gkt1387 (2014).
Luco, R. F. et al. Regulation of alternative splicing by histone modifications. Science 327, 996–1000, doi: 10.1126/science.1184208 (2010).
Roux, J. C. et al. Modification of Mecp2 dosage alters axonal transport through the Huntingtin/Hap1 pathway. Neurobiol Dis 45, 786–795, doi: 10.1016/j.nbd.2011.11.002 (2012).
Zhou, Z. et al. Brain-specific phosphorylation of MeCP2 regulates activity-dependent Bdnf transcription, dendritic growth, and spine maturation. Neuron 52, 255–269, doi: S0896-6273(06)00775-6 10.1016/j.neuron.2006.09.037 (2006).
Long, S. W., Ooi, J. Y., Yau, P. M. & Jones, P. L. A brain-derived MeCP2 complex supports a role for MeCP2 in RNA processing. Bioscience reports 31, 333–343, doi: 10.1042/BSR20100124 (2011).
Cheng, J. et al. SUMOylation of MeCP2 is essential for transcriptional repression and hippocampal synapse development. J Neurochem 128, 798–806, doi: 10.1111/jnc.12523 (2014).
Anders, S., Reyes, A. & Huber, W. Detecting differential usage of exons from RNA-seq data. Genome research 22, 2008–2017, doi: 10.1101/gr.133744.111 (2012).
Cohen, S. et al. Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function. Neuron 72, 72–85, doi: 10.1016/j.neuron.2011.08.022 (2011).
Kim, T. K. et al. Widespread transcription at neuronal activity-regulated enhancers. Nature 465, 182–U165, doi: 10.1038/nature09033 (2010).
Hahn, M. A. et al. Dynamics of 5-Hydroxymethylcytosine and Chromatin Marks in Mammalian Neurogenesis. Cell Reports 3, 291–300, doi: 10.1016/j.celrep.2013.01.011 (2013).
Zhang, Y. et al. Model-based Analysis of ChIP-Seq (MACS). Genome biology 9, doi: Artn R137 10.1186/Gb-2008-9-9-R137 (2008).
Darnell, J. C. et al. FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism. Cell 146, 247–261, doi: 10.1016/j.cell.2011.06.013 (2011).
Croning, M. D., Marshall, M. C., McLaren, P., Armstrong, J. D. & Grant, S. G. G2Cdb: the Genes to Cognition database. Nucleic Acids Res 37, D846–851, doi: 10.1093/nar/gkn700 (2009).
Basu, S. N., Kollu, R. & Banerjee-Basu, S. AutDB: a gene reference resource for autism research. Nucleic Acids Res 37, D832–836, doi: 10.1093/nar/gkn835 (2009).
This work was supported by CAS Strategic Priority Research Program (XDB02050400), NSFC Grants (#91432111, #81527901) to Z.Q. ECNU National 985 Project grant and Shanghai Pujiang Talent Grant. Supported by Shanghai Sailing Program (15YF1414200) and The Knowledge Innovation Program of CAS (2014KIP205) to T.-L.C.
The authors declare no competing financial interests.
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Cheng, TL., Chen, J., Wan, H. et al. Regulation of mRNA splicing by MeCP2 via epigenetic modifications in the brain. Sci Rep 7, 42790 (2017). https://doi.org/10.1038/srep42790
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