Figure 1: Identification of MeCP2-associated proteins by mass spectrometry analysis and functional annotations. | Scientific Reports

Figure 1: Identification of MeCP2-associated proteins by mass spectrometry analysis and functional annotations.

From: Regulation of mRNA splicing by MeCP2 via epigenetic modifications in the brain

Figure 1

(a) Rat Mecp2 genome locus and TALEN-targeting region design. (b) Sequence of the Mecp2 locus targeted by TALEN-based gene targeting from WT and Mecp2-null rat. Corresponding amino acid sequence was annotated. (c) MeCP2 expression in Mecp2-null rat cortex. Total protein lysates were extracted from Mecp2-null rat cortex and related WT littermate cortex and then analyzed by western blot. (d) Experimental workflow for immunoprecipitation and mass spectrometry analysis of MeCP2-associated proteins in 293 T cells, mouse cortical neurons and rat cortex. (e) Number of MeCP2-associated proteins identified by mass spectrometry in 293 T cells, mouse cortical neurons and rat cortex. (f) Mouse GO analysis for MeCP2-associated proteins identified in both cultured mouse cortical neurons and rat cortex. (g) Protein-protein interaction network analysis for 58 MeCP2-binding proteins identified in both cultured mouse cortical neurons and rat cortex. (h) Protein-protein interaction network analysis for 37 MeCP2-binding proteins identified in 293 T cells which were also detected in either mouse cortical neurons or in rat cortical tissues.

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