Figure 5 : For the real dataset, detected differential gene expression was unbalanced, specially after using MedianCD and SVCD normalization and for the treatments with more DEGs (Fig. 3a).

From: Variation-preserving normalization unveils blind spots in gene expression profiling

Figure 5

Panels show the balance of differential gene expression, for each treatment compared to the corresponding control, obtained after Median normalization (a), Quantile normalization (b), MedianCD normalization (c), and SVCD normalization (d). Each point represents the balance of differential gene expression, (, same number of over- and under-expressed genes; , all DEGs over-expressed; , 75% DEGs under-expressed). Points are colored by treatment, with the same color code as in Figs 1 and 4. All panels have the same order of treatments as in Figs 3a and 4, i.e. in increasing number of DEGs identified with SVCD normalization (Supplementary Table 2). Dashed horizontal lines indicate references for balanced differential gene expression ().