Microfluidic cell isolation technology for drug testing of single tumor cells and their clusters

Drug assays with patient-derived cells such as circulating tumor cells requires manipulating small sample volumes without loss of rare disease-causing cells. Here, we report an effective technology for isolating and analyzing individual tumor cells and their clusters from minute sample volumes using an optimized microfluidic device integrated with pipettes. The method involves using hand pipetting to create an array of cell-laden nanoliter-sized droplets immobilized in a microfluidic device without loss of tumor cells during the pipetting process. Using this technology, we demonstrate single-cell analysis of tumor cell response to the chemotherapy drug doxorubicin. We find that even though individual tumor cells display diverse uptake profiles of the drug, the onset of apoptosis is determined by accumulation of a critical intracellular concentration of doxorubicin. Experiments with clusters of tumor cells compartmentalized in microfluidic drops reveal that cells within a cluster have higher viability than their single-cell counterparts when exposed to doxorubicin. This result suggests that circulating tumor cell clusters might be able to better survive chemotherapy drug treatment. Our technology is a promising tool for understanding tumor cell-drug interactions in patient-derived samples including rare cells.


Drug assays with patient-derived cells such as circulating tumor cells requires manipulating small sample volumes without loss of rare disease-causing cells. Here, we report an effective technology for isolating and analyzing individual tumor cells and their clusters from minute sample volumes using an optimized microfluidic device integrated with pipettes. The method involves using hand pipetting to create an array of cell-laden nanoliter-sized droplets immobilized in a microfluidic device without loss of tumor cells during the pipetting process. Using this technology, we demonstrate single-cell analysis of tumor cell response to the chemotherapy drug doxorubicin. We find that even though individual tumor cells display diverse uptake profiles of the drug, the onset of apoptosis is determined by accumulation of a critical intracellular concentration of doxorubicin. Experiments with clusters of tumor cells compartmentalized in microfluidic drops reveal that cells within a cluster have higher viability than their single-cell counterparts when exposed to doxorubicin. This result suggests that circulating tumor cell clusters might be able to better survive chemotherapy drug treatment. Our technology is a promising tool for understanding tumor cell-drug interactions in patient-derived samples including rare cells.
Understanding interactions between tumor cells and drugs is important for discovery of new oncogenic targets 1-3 , development of cancer drug candidates 4 and generating insights into the mechanisms of chemotherapy drug resistance 5,6 . Despite significant advances in understanding mechanisms of tumor development and progression 7,8 , the current clinical success rate of lead cancer drug candidates remains below 5%, significantly lower than that of cardiovascular (~20%) and infectious diseases (~17%) therapies 2 . Likewise, chemotherapy drug resistance is believed to be responsible for treatment failure in more than 90% patients with metastatic disease 9 , motivating the need to better understand in a patient-specific manner how chemotherapy drugs interact with cancer cells so that personalized treatments can be designed.
Identifying new drug targets or compounds and the molecular mechanisms of chemotherapy resistance requires preclinical models that adequately capture the complexities of cancer. Established tissue culture cell lines are often used as an in vitro model of cancer [10][11][12] , but these cell lines display amplified proliferation, transformed sensitivity to chemotherapy, and reduced cellular heterogeneity [13][14][15] . As a result, there has been a growing interest in conducting drug studies with patient-derived cells including human tissues and biofluids as a superior model of the in vivo situation 10,13,16 . Patient-derived cells are expected to better predict patient outcomes as they have been found to be more heterogeneous, with reduced proliferation rates and enhanced resistance to chemotherapy compared to established cell culture lines 17 .
Among the patient-derived cells, circulating tumor cells (CTCs) isolated from the blood of cancer patients offer a rich test bed for drug development and chemoresistance assays because (i) CTCs and their clusters (of typically 2-50 cells [18][19][20] ) provide a compelling mechanism for metastasis 19 , with clusters having significantly more metastatic potential 19 , (ii) molecular profiling of CTCs shows they are very heterogeneous, similar to cells in a primary tumor, and share some common genetic mutations 21,22 , (iii) blood samples are less invasive compared to tissue biopsies and are easier to procure, and (iv) they can be sampled longitudinally for identifying drug resistance. Thus, CTCs are an attractive candidate for drug discovery and probing mechanisms of chemoresistance.
The promise of CTCs for drug investigations has been complemented by an explosion in the number of available microfluidic technologies available for isolating CTCs, even though they are present in low counts, typically 1-100 cells per mL of blood 23 . A number of microfluidic techniques are capable of antibody-based capture and release of CTCs [24][25][26] . In addition to these immunocapture methods, several label-free methods based on size and deformability also exist to separate CTCs 25,[27][28][29] . More recently, clusters of CTCs have also been isolated using microfluidic approaches 30 .
The advent of numerous technologies for efficiently isolating CTCs opens unique opportunities for using CTCs for drug discovery and probing drug resistance. However, technical hurdles still exist for conducting drug investigations using CTCs. First, even though microfluidic technologies are available for efficiently isolating and collecting CTCs, conducting drug assays downstream can be challenging due to potential loss of the rare cells while handling them using pipettes and multiwell plates. Second, although ex vivo culture methods are beginning to emerge to culture CTCs for drug assays [31][32][33] , the molecular heterogeneity of individual CTCs and clusters is often lost during the bulk expansion process making it difficult to identify drug resistant cells.
In this study, we present a pipette-based microfluidic cell isolation (MCI) technology that is capable of conducting single cell resolution drug assays with a small number of tumor cells or their clusters present in small sample volumes (e.g. 10-100 cells in 10 μ L). The method is based on digitizing the sample volume containing tumor cells into an array of nanoliter-scale droplets by simply using a pipette and a microfluidic device. The sample digitization occurs in the device in such a way that an array of static droplets is created in which tumor cells and their clusters are isolated. This approach also allows automated imaging of tumor cells stored in the droplets.
To establish proof-of-principle of our pipette-based MCI method for CTC research, we use breast cancer cells (MCF-7) and a chemotherapy drug, doxorubicin. Doxorubicin is an FDA approved cytotoxic drug used widely in cancer chemotherapy 34,35 and this was chosen in this study as it is the most active single agent available for the treatment of breast cancer 36 . Using this system, we demonstrate that (i) individual MCF-7 cells can be isolated without any loss during the pipetting and digitization steps (ii) our method can isolate clustered tumor cells containing 2-22 cells per cluster (iii) the uptake profile of doxorubicin is heterogeneous in individual tumor cells and that a critical amount of intracellular uptake of doxorubicin determines the onset of apoptosis (iv) clustered tumor cells show a higher viability than their single-cell counterparts when exposed to doxorubicin suggesting that being in a cluster can alter a cancer cell's response to chemotherapy drugs. The overall viability of clusters might be improved due to the coordinated resistivity provided by the group of cells. Overall, our MCI technology is a promising tool for chemotherapy drug testing on CTCs and their clusters.

Results and Discussion
Basic description of the MCI technology. In recent years, drop-based microfluidics has emerged as a powerful technology to compartmentalize cells in volumes down to picoliters [37][38][39] . The standard practice has been to encapsulate cells within trains of individual droplets produced in T-junction or flow-focusing devices, using syringe pumps. Subsequently, these droplets are stored in a variety of ways including on-chip incubation chambers/traps 40,41 and off-chip incubation 39 . Although such methods have been shown to efficiently isolate individual cells for single-cell assays 39 , these techniques are incompatible with the needs of rare cell handling and manipulation, where only 10-100 cells are present in about 10-100 μ L of sample volume. Specific reasons for the incompatibility include (i) establishing a stable flow and consistent drop production by syringe pumps requires stabilization time, which necessitates the use of more sample volume, typically > 0.1 mL (ii) the inevitable presence of dead volumes resulting in not all of the sample being digitized into droplets and (iii) finally, there is a chance of losing cells in the syringe and tubing due to sedimentation and non-specific adhesion. Thus, the standard practice of using syringe pumps for droplet generation is ill suited for compartmentalizing rare cell samples.
We describe a simple method that resolves the above issues making it particularly suited for assaying rare cells. As shown in Fig. 1, our MCI technology involves using a pipette as a fluid-handling tool 42 in conjunction with a microfluidic device that is capable of storing or parking arrays of nanoliter droplets. This approach is much more simplified than techniques presented in our previous work, where we used syringe pumps to create droplet arrays 43,44 . The current technique involves a highly robust method of creating an off-chip oil-sample cartridge in a pipette tip by sequentially aspirating oil and sample, followed by a single step dispensing into the microfluidic device that has been prefilled with oil.
The mechanism of sample digitization is dictated by the geometry of the microfluidic parking network [43][44][45] . As shown in Fig. 1b, the device contains several interconnected parking loops, with each loop containing a trap chamber and a bypass channel. As the plug travels through the microfluidic device, the long aqueous plug breaks at the junctions of the parking loop, leaving remnants of the plug parked in the trap chambers. The manual pipetting does not introduce large error in droplet volumes since the trapped volume is preset by the size of the trap. This process creates an array of sixty drops of uniform volumes (30 nL in Fig. 1b, with a polydispersity of < 5%) in less than 30 sec, using only 2 μ L sample. Thus, our MCI approach enables the production of static droplet arrays (SDAs) with 90% of the sample being digitized and stored on the device, by simply using a pipette and avoiding the complications of syringe pumps.

Isolation of single tumor cells (Sl-TC).
Given that we can efficiently digitize the sample volume, we tested our approach as to whether (i) cells can be stored in the device without loss during sample manipulation, and (ii) single cells can be efficiently isolated in individual drops. This testing is important to ensure that rare CTCs can be isolated individually without cell loss. As a proof-of-principle, we used the human breast cancer cell line MCF-7. Experimental protocols used to test the efficacy of isolating MCF-7 cells are schematically illustrated in Fig. 2a. As shown in Fig. 2b, the data obtained from the two methods are in very good agreement. For example, the MCI method has 0% loss for the sample with 250 cells/10 μ l, 5% loss for the sample with 100 cells/10 μ l and maximum 15% loss for the sample with 500 cells/10 μ l. Thus, the MCI technology showed 85-100% effectiveness for loss-less separation of cells from small sample volumes. Our results suggest that there is little or no adhesion of cells to pipette tips. However, a recent study suggests that nonspecific cell adhesion can occur due to the hydrophobic nature of most commercially available pipette tips 46 . We believe that non-specific adhesion of cells to pipette tips is mitigated in the MCI approach due to the presence of a thin biocompatible oil film in the pipette tip, which prevents cell loss during pipette-based fluid handling.
We also tested whether our method for single cell isolation produces Poisson statistics. Previously, single particle encapsulation in microfluidic droplets using T-junction and flow-focusing devices 40 have shown that the encapsulation process follows a Poison distribution: x where x is the number of cells present in the droplet and λ is the expected average number of cells per droplet, which is adjusted by controlling the stock cell density.
In microfluidic droplet generation methods such as T and cross-junction, cells are encapsulated at sub-second time scales, however, in our case the timescale for encapsulating cells in droplets is tens of seconds (see Fig. 1b) causing potential cell sedimentation issues 44 , which might alter the cell encapsulation statistics from a Poisson distribution. To test the applicability of Poisson statistics for the MCI method, we used three different cell samples (500, 250, 100 cells/10 μ l). For these cell concentrations, the expected average numbers of cells per droplet (λ ) are 1.5, 0.75, and 0.3. The probability distribution P(x) was calculated by counting the number of cells per droplet (x) from a total of 60 drops for each cell sample. Using Eqn. (1), we also estimated the theoretical Poisson distribution considering the number of cells/per trap (x = 0, 1, 2, 3, 4… ..) for each expected average number of cells per droplet (λ ). Figure 2c shows the statistics of the isolation process from the experiment (symbols) and it's comparison to theoretical prediction (dashed lines). We find that the experimental cell distribution closely follows Poisson statistics and about 25-35% drops in the microfluidic device contain single cells.
Overall, the MCI technology is capable of efficient isolation of individual cells from minute samples that contain a small number of cells, without much cell loss. The MCI approach addresses the current issues associated with handling rare cells using pump-based droplet microfluidics. Likewise, it outperforms traditional pipette and multiwell plate fluid handing method that have difficulty in handling nanoliter volumes and isolating single cells. Thus, the MCI technology has significant potential for handling small number of cells in minute samples that is typical for assays with isolated CTCs or primary human cells.

Influence of microfluidic drop environment on tumor cell viability.
A potential concern of the MCI technology for drug testing is that the drop environment might adversely affect the viability of tumor cell suspension. For example, MCF-7 cells that are cultured in the laboratory in the adherent state, may undergo anoikis in the microfluidic drop environment, and therefore potentially introduce bias in our drug testing data. To address this concern, we conducted microfluidic experiments with CCRF-CEM leukemia cells whose natural as well as laboratory culture environment is liquid media. This allowed us to compare the level of anoikis of these CEM cells with that of MCF-7 cells incubated in microfluidic droplets. As shown in Fig. 3a, both the MCF-7 and CEM cells showed greater than 90% viability in droplets across 24 hours. Statistical analysis reveals that the percentage viability between the two cells lines is indistinguishable, suggesting that the level of anoikis is not very different between MCF-7 and CEM.
We also conducted long-term viability studies for up to 7 days, with MCF-7 cells in the microfluidic drops (see Fig. 3b). We observed that more than 90% cells are viable up to 72 hrs. On days 4, 5, 6 and 7, we found that cell viability is 75, 62, 43 and 21% respectively. We also recorded changes in drop volume due to evaporation, and find that the change in volume in 72 hrs is less than 10%, after which the drop volume remains almost unchanged (Fig. 3c). Thus, significant reduction in cell viability after day 3 is potentially due to lack of nutrients and anoikis. These results suggest that cells can be cultured successfully for drug testing up to 72 hrs, using the MCI technology.
Comparison of performance of MCI technology against multiwell plates. To assess the performance of our MCI technology vis-à-vis conventional method, we conducted a dose response assay using multiwell plates and the MCI technique, and compared the two methods. Muliwell plates are the most commonly used approach for conducting cell-based drug assays, Fig. 4 shows the comparison between the two approaches. We find that the dose-response curve obtained from the MCI technique is statistically indistinguishable from that of the 96-well plate data. This result suggests that the performance of our approach is comparable to that of multiwell plates, but with marked advantages of (i) isolating single cells without much cell loss and (ii) conducting dose-response assays with ~O(10 2 ) cells, rather than the O(10 4 ) cells that are typically used in multi-well plates.

Kinetics of Dox uptake and its relationship to tumor cell apoptosis. Given that the MCI technology
has the capacity to isolate single cells and the microfluidic drop environment does not adversely affect cell viability, we studied the kinetics of uptake of doxorubicin by individual breast cancer cells. Doxorubicin can induce cancer cell death through different mechanisms: (i) inhibition of topoisomerase II 47 ; (ii) intercalation to base pairs of the DNA double helix, blocking the synthesis of DNA and RNA, and DNA strand scission 48 ; (iii) binding to cellular membranes to alter fluidity and ion transport; and (iv) generation of semiquinone free radicals and oxygen free radicals through an enzyme-mediated reductive process 49 .
Despite the ability of Dox to induce cell death through several mechanisms, breast cancer cells (MCF-7) can still show drug resistance to Dox after first chemotherapy 50,51 . The uptake rate of a drug by cancer cells is usually controlled by the resistivity of the cell population 5,52 . As a result, there is a need to identify which cells in a population are resistant to Dox uptake and how the uptake kinetics correlates with cellular apoptosis. Such investigations are difficult to pursue with bulk drug assays. Microfluidic approaches are suitable for single cell drug assays 53,54 , however, a survey of literature shows that Dox uptake kinetics has not been correlated to apoptotic susceptibility in MCF-7 cells. Given that we have demonstrated effective isolation of single cells using MCI technology, here we study single-cell kinetics of Dox uptake, identify Dox sensitive and insensitive cells and establish a correlation between accumulation of Dox and apoptotic susceptibility.
To quantify Dox uptake, the amount of auto fluorescence due to presence of Dox in the nucleus of individual cells was measured from droplets that contained only single cells (n = 118) over a period of 9 hrs. Similar to previous studies 53, 55 , we observed significant heterogeneity among MCF-7 cells in terms of Dox uptake and retention. Figure 5a shows representative images of Dox accumulation in the nuclei of individual tumor cells (in red) co-stained with Annexin V (in green) for apoptosis. In general, we observe that cells that uptake high amounts of Dox undergo apoptosis. Importantly, we found eight different Dox uptake and retention profiles depending on how the Dox concentration, [C] Dox , changes between different arbitrary time points of 1, 3, 6 and 9 hrs. As shown in Fig. 5b Given the heterogeneous uptake profiles, we sought to investigate the correlation between Dox uptake and apoptotic susceptibility. We used two-color fluorescence imaging to establish this relationship and representative images are shown in Fig. 5a. We evaluated if there was a critical Dox uptake concentration at which tumor cells undergo apoptosis, irrespective of whether they uptake the drug slowly or rapidly. To address this, in Fig. 5c we plot the [C] Dox distribution at different time points and identified the [C*] Dox ≈ 825-900 at which apoptosis is initiated (vertical lines in Fig. 5c). Interestingly, we find that the critical intracellular Dox concentration needed to trigger apoptosis decreases only slightly with increase in incubation time. However, the percentage of apoptotic cells increases significantly from 35% to 58% at time points of 1 hr and 9 hr respectively. Taken together, these results suggest that a critical intracellular concentration of the chemotherapy drug Doxorubicin is needed to induce cell death in MCF-7 breast tumor cells and cells that do not uptake the critical Dox concentration survive.
The surviving and non-surviving cells show interesting uptake patterns. The non-surviving cells undergoing apoptosis uptake a Dox concentration higher than [C*] Dox at initial time points, but the Dox concentration decreases towards the later time points. The surviving cells mostly follow the d-i-i, d-d-i, i-i-i, and i-d-i patterns, indicating that towards later time points the Dox concentration increases, but still remaining mostly below [C*] Dox .

Isolation and drug response of clustered tumor cells (Cl-TC). Most of the CTCs in patients are
found as single cells. However, a very small fraction (2-5%) of CTCs are found as clusters with the number of cells within a cluster ranging from 2-50 19,30 . These clusters show 50 times more metastatic potential than single CTCs 19,[56][57][58] . A major issue with handling CTC clusters is their sensitivity to rupture under shear. For example, the CTC-cluster chip uses an estimated shear rate of ≈ 700 s −1 to isolate tumor cell clusters from cancer patient blood 30 . The shear rate during the pipetting and loading/trapping process in the MCI technology is about 10-20 s −1 , making it a promising candidate for handling clusters of tumor cells. We therefore generated clusters of MCF-7 tumor cells 30 and tested the capability of the MCI method to efficiently encapsulate these clusters in the droplet array and conduct drug assays. We focused on addressing two questions: (i) What is the probability of generating clusters containing a given number of cells (ii) Do single tumor cells show different drug response than cells present in clusters?
Size distribution of tumor cell clusters. To generate clusters, we chose a stock cell density such that the average number of cells per droplet, λ = 10. The detailed protocol for preparing clusters is discussed in the Experimental section. Using this protocol, we trapped both clustered tumor cells (Cl-TC) and single tumor cells   (Sl-TC) in drops (Fig. 6a, top). As shown in the images of Fig. 6a, we obtain clusters with N c = 2-22 cells, where N c is the number of tumor cells in a cluster. To characterize the probability distribution of obtaining a cluster with a given N c , we interrogated 360 drops (from 6 microfluidic devices) and the data is shown in Fig. 6b. We find that clusters with N c = 2 and 3 are the most predominant with probabilities of 30% and 26% respectively. Tumor cell clusters with N c ≥ 4 have a lower probability of occurrence, about 1-12%. In addition, we note that the cluster size probability distribution depends on how cells detach during trypsinization and the extent of pipette-induced mixing, since the cluster distribution obtained from each of the 6 devices were not the same (data not shown). Figure 7a shows representative images of clusters of different sizes that have been exposed to Dox. In general, we find that a significant number of clusters contain live cells even after 20 hrs of drug exposure. To investigate whether isolated cells show a different drug response than cells present in clusters, we tested the response of Dox on two different batches as We assessed cell viability at 0, 10, 20 and 24 hrs in both batches. To test whether the clustered tumor cells show different drug response than single cells, we performed standard unpaired t-test for time points 10, 20 and 24 hrs. At these three time points, clustered tumor cells showed statistically higher viability than that of the independently generated single cell population as well as the co-existing single cell population. We checked for anoikis with clusters, and we did find it to contribute significantly to cell death (see Fig. S1). Thus, even though the single and the clustered cells were from the same population, the number of cells surviving drug exposure depended on whether they were present as individuals or clusters.

Drug response of single tumor cell (Sl-TC) vs. clustered tumor cell (Cl-TC).
The Dox response of tumor cell clusters is different from that of single cells probably because the uptake kinetics of the drug could be different for a group of cells than individuals, especially when they are confined in microfluidic droplets. It is also possible that the overall drug resistivity of clustered tumor cells might be different compared to the resistivity of individual cells. From a cancer metastasis perspective, our result suggests that a CTC cluster has a better chance of surviving chemotherapy drug treatment than a single CTC.

Conclusions
Patient-derived cells from human tissues and biofluids are increasingly being used to predict patient outcomes as they are a superior model of the in vivo situation 10,13,16 . For example, recent advances to separate CTCs from blood have opened up opportunities for downstream assays of rare CTCs. However, to harness the full benefits of these downstream assays of rare CTCs requires manipulating a small number of cells without their loss during handling. Even loss of one diseased CTC from a pool of cells, might incorrectly predict the patient outcome. In this context, our simple MCI method offers benefits of i) reproducible sample discretization, ii) control over distribution of minute amount of cells/clusters without much loss of cells, iii) assessing heterogeneity at single cell level, iv) easy identification of drug response and, v) long time observation of intracellular events while preserving identity of each cell.
To demonstrate the power of the method, we have conducted proof-of-principle drug studies and provided useful insights into the response of single isolated tumor cells and clusters of tumor cells. Importantly, we have shown that individual cancer cells show a rich variety of drug uptake patterns and that clusters are more resistant to drug treatment. These results obtained from in vitro tumor cell lines need to be validated with actual CTCs from patient samples. The capabilities of the MCI technology can be further expanded to replace media from the droplets 44 for long term drug studies and also characterize the influence of drugs on co-culture of cells (e.g. different proportions of tumor and stromal fibroblast cells 59 .

Methods
Fabrication of microfluidic devices. The design of the devices used in this study is identical to that used in our previous works 43,44 . Briefly, the device contains a microfluidic network of 60 loops (Fig. 1). Each loop has a bypass channel and a lower branch containing a trap that can park a droplet of volume 30 nL (Fig. 1a). In this microfluidic device 44 , all the channel widths and diameters of the trap chambers are 200 μ m and 450 μ m respectively. The height of all the features in the device is 170 μ m. Microfluidic devices were fabricated using soft lithography 60 . Polydimethyl siloxane (PDMS) prepolymer and curing agent were mixed in a 10:1 ratio, degassed and poured on the mold and cured for a minimum of two hours at 65 °C. Subsequently the PDMS replica was cut with a scalpel and peeled. Inlet and outlet reservoirs were defined by punching holes. The replica was placed on a PDMS coated glass slide that was partially cured in the oven at 65 °C for 10 minutes. The entire assembly was further cured at 65 °C for two hours to create an irreversible seal.
Cell culture. The human breast cancer cell line (MCF-7) obtained from American Tissue and Culture Collection (ATCC, Manassas, VA, USA) was maintained and grown in DMEM medium containing 10% fetal bovine serum, 1% Sodium Pyruvate, and 1% Penicillin/Streptomycin at 37 °C in 5% CO 2 . Cell culture chemicals were purchased from Fisher Scientific. Different cell densities of MCF-7 cells were used as samples in the cell assay experiments. For all experiments, the initial cell concentration was 1 × 10 6 cells/ml and desired final concentrations were achieved by diluting with DMEM media.
Preparation of cell samples. To produce the cell samples for single tumor cell assays, we used a cell concentration of 0.35 × 10 6 cells/ml. The dilution and staining process were done by pipette mixing multiple times (at least 10 times) followed by vortex mixing to ensure that no clustered cells are disrupted.
To produce clustered tumor cell clusters (Cl-TC), we used the same concentration as the single cell assays, but we modified the mixing process to preserve cell clusters/groups. The total dilution and staining process were done by only three times mixing with pipette. This ensured mixing without significantly disrupting cell clusters. However, in this process each of the traps of the microfluidic device contained a mixture of clustered tumor cells and single tumor cells.
Drug assays. We conducted drug assays on the tumor cells in our microfluidic device, using the anti-cancer drug doxorubicin hydrochloride (Dox) (Sigma Aldrich). For all assays, Dox was used at a concentration of 10 μ M. Initially, Dox was mixed with distilled water to make a 1 mM stock solution of drug and stored at 4 °C. For all experiments, stock solution of Dox was freshly diluted with DMEM media to a concentration of 10 μ M. For drug assays, Dox was mixed with the cell samples and immediately loaded into the microfluidic device and imaged. The drug mixing and loading process were done within 2 minutes. In between different imaging time points the microfluidic devices were incubated at 37 °C in 5% CO 2 .
Scientific RepoRts | 7:41707 | DOI: 10.1038/srep41707 Pipetting procedure for loading cell samples. All the samples including cell suspensions and drug treated cells were handled in the microfluidic device using a multi-channel VIAFLO Electronic Pipette (0.5-12.5 μ L, INTEGRA Biosciences Corp., NH, USA) and Grip-tips pipette tips (12.5 μ L, INTEGRA Biosciences Corp., NH, USA). We tested several pipette tips (10 μ L, 12.5 μ L, 100 μ L, 200 μ L) and dispensed volumes (5 μ L-10 μ L) on a manual pipettor. We found that 12.5 μ L, pipette tips with dispensed volumes of 7 μ L worked the best in terms of uniform trapped volumes. The microfluidic devices were primed with a carrier fluid (FC-40, Sigma Aldrich) containing 0.001% w/w biocompatible fluorosurfactant (KrytoxFSH-PEG600-KrytoxFSH, RAN Biotechnologies, Inc., MA). Then, an off-chip oil-sample cartridge was prepared in the pipette tip using the multichannel automated pipette. The cartridge contains 5 μ L of oil and 2 μ L of sample. This oil-sample cartridge was then injected, using the electronic pipette, into the device that was prefilled with carrier fluid.
Viability and apoptosis detection. LIVE/DEAD ® Cell Imaging Kit (Life technologies) was used to quantify cell viability. The 'Live' and 'Dead' parts of the stain were mixed following the manufacturer protocol. The mixed solution was diluted 3 times with cell media. This 3x-diluted solution was mixed with cell samples at 50/50 volume ratio to stain the cells. NucBlue ® Live ReadyProbes ® Reagent (Life technologies) were used to identify cell nuclei to accurately quantify cell counts.
Apoptosis was detected using Alexa Fuor ® 488 Annexin V (Thermo Fisher Scientific). The dilution of cell samples and Dox were done with 1X Annexin-binding buffer (Thermo Fisher Scientific). Dox was mixed with the cell samples as described for other drug assays at a concentration of 10 μ M. 25 μ l of Alexa Fuor ® 488 Annexin V (Thermo Fisher Scientific) was added to 100 μ l cell-drug samples as an early-stage apoptosis marker. The cell sample with the Alexa Fuor ® 488 Annexin V was incubated at room temperature for 15 min, loaded to the microfluidic device and then incubated at 37 °C in 5% CO 2 for 45 mins. Fluorescence images (FITC and TRITC) were taken at time points of 1, 3, 6 and 9 hrs. In between imaging, the devices were incubated at 37 °C in 5% CO 2 .
Image acquisition and processing. Brightfield and fluorescence images of cells in droplets were captured using Olympus IX 81 microscope (Massachusetts, USA) equipped with a Thorlab automated stage (New Jersey, USA) controlled by SlideBook 6.1 (3i Intelligent Imaging Innovations, Inc., Denver, USA). Images were acquired with a Hamamatsu digital camera (ImagEM X2 EM-CCD, New Jersey, USA), 10-60x objectives and standard FITC/DAPI/TRITC filters. The exposure times used in the experiments ranged from 20-430 ms.
The uptake of doxorubicin i. e. concentration of Dox, [C] Dox , was quantified from the time-lapse images of each cell. Image processing was done with ImageJ (http://rsb.info.nih.gov/ij/). The [C] dox in arbitrary units (a.u.) was calculated after background correction by the following equation, [C] dox = Mean fluorescence of cell − Mean fluorescence of background. A composite image from FITC (live) and TRITC (dead) filters was used to assess live/dead cells in ImageJ.