Figure 4: The role of VEGFR2 in VEGF-induced tumour stemness in pHCCs. | Scientific Reports

Figure 4: The role of VEGFR2 in VEGF-induced tumour stemness in pHCCs.

From: CD133+ cancer stem cells promoted by VEGF accelerate the recurrence of hepatocellular carcinoma

Figure 4

Two pHCCs were isolated from two HCC patients (#1 and #2) and then stimulated by VEGF for 72 hours. (a) Representative immunofluorescence images showing the expression of CD133, p-VEGFR2 and Nanog in two pHCCs (#1 and #2). Scale bars = 10 μm. For pHCCs #1, red and green secondary antibodies were used to detect CD133 and p-VEGFR2, respectively. For pHCC #2, green and red secondary antibodies were used to detect CD133 and p-VEGFR2, respectively. (b) The levels of CD133+ CSCs and CD133+, p-VEGFR2+, Nanog+ CSCs in (a). (c) Immunoblot detection of CD133, VEGFR2, p-VEGFR2 and Nanog in two pHCCs (#1 and #2). shRNA (sh)-mediated interference was used to produce two VEGFR2 knockdown cell line from the two pHCCs. The efficiency of the knockdown is shown in supplementary Figure 1b. (d) Immunoblot assay demonstrating the effect of VEGF stimulus on the levels of CD133 and Nanog in two pHCCs with or without VEGFR2 knockdown. (e) Spheres formation assays demonstrating the effect of VEGF stimulus on self-renewal ability of pHCCs with or without VEGFR2 knockdown. The values are shown as the mean ± SEM of triplicates. *p < 0.05.

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