(A,B) Observation of tolerance to ampicillin (10 μg) in tbl3a. (C,D) Same strain (tbl3a) exposed to Kanamycin (7.5 μg). No tolerance is observed (See Fig. S10 for empirical determination that the kanamycin concentration drops below the MIC) Disk diameter: 6 mm. (E) Time-kill curves of the same strain in Kanamycin 100 μg/ml (beige) and Ampicillin 100 g/ml (red). The large fraction of survivals in Ampicillin (12%, after 2.6 hours) was predicted by the late colonies appearance, and is absent in Kanamycin (1.4E-4%, after 2.6 hours).