Chemically-activatable alkyne-tagged probe for imaging microdomains in lipid bilayer membranes

A chemically-activatable alkynyl steroid analogue probe has been synthesized for visualizing the lipid raft membrane domains by Raman microscopy. The Raman probe, in which ring A of its steroid backbone is replaced with an alkynyl group, was designed to enable activation of the alkyne signal through the Eschenmoser-Tanabe fragmentation reaction of the oxidized cholesterol precursor in lipid bilayer membranes. The alkynyl steroid analogue was observed to form liquid-ordered raft-like domains on a model giant-liposome system in a similar manner as cholesterol, and the large alkyne signal of the accumulated probe at 2120 cm−1 was mapped on the microdomains with a Raman microscope. The alkyne moiety of the probe was confirmed to be converted from the α,β-epoxy ketone group of its precursor by reaction with p-toluensulfonyl hydrazine under a mild condition. Through the reaction, the alkyne signal of the probe was activated on the lipid bilayer membrane of liposomes. Furthermore, the signal activation of the probe was also detected on living cells by stimulated Raman scattering microscopy. The ring-A-opened alkyne steroid analogue, thus, provides a first chemically-activatable Raman probe as a promising tool for potentially unravelling the intracellular formation and trafficking of cholesterol-rich microdomains.

Alkynes have recently attracted attention as a label to replace bulky charged fluorescent dyes. Alkynes are much smaller and they do not induce any intermolecular electrostatic interaction. In addition, carbon-carbon triple (C≡ C) bonds in alkyne compounds can be used effectively for Raman imaging [13][14][15] . Raman microscopy can be used to image chemical moieties of intracellular molecules by recording the vibrational signatures of their characteristic bonds with Raman scattering of less-cytotoxic visible light. Moieties such as alkyne bonds and carbon-deuterium bonds, which rarely occur naturally, generate strong Raman signals in a region of the spectrum that is typically silent for cells (1800− 2800 cm −1 ); that is, at frequencies at which most intracellular biomolecules show no Raman signals. Accordingly, alkyne-tagged small molecules can be visualized through the use of Raman microscopy without significant impact on the intrinsic properties of such compounds in cells [13][14][15] . Recently, a cholesterol analogue probe including C≡ C bonds was reported to be utilized for visualizing cholesterol storage in living cells and C. elegans 16 . In this pioneering study, the static intracellular distribution of the cholesterol probe was observed by Raman imaging, comparable to those of conventional DHE by fluorescence imaging 11,16 .
Stimuli-responsive activatable probe has been reported to be a powerful tool for unravelling dynamic distribution changes of biomolecules [17][18][19][20][21] . A wide variety of activatable fluorescent probes that response to various stimuli such as light 17 , ions 18,19 , chemicals 20 and enzymes 21 have been actively studied. By using such probes, the labelled molecules which go through light-irradiated areas or specified environments such as low-pH, highly-reduced and enzyme-rich sites could be selectively visualized. In the latter cases, the selective visualization of the probes has yielded new insights into the microenvironment of the pathological regions, leading to development of new drug delivery and diagnosis systems [18][19][20][21] . Owing to the benefits of alkyne tag Raman imaging, an activatable alkyne probe would be potentially more promising tool for real-time observation in living systems. But, to our knowledge, no alkyne probes that are activated by external stimuli have been reported. Therefore, we tried to develop an activatable Raman probe for visualizing the dynamics of biomolecules. Herein, based on Eschenmoser-Tanabe fragmentation reaction, a chemically-activatable Raman probe for visualizing the cholesterol-rich microdomains is reported. Similar to cholesterol, this probe leads to the formation of liquid-ordered cholesterol-rich domains on the lipid bilayer membrane through preferential accumulation to the domains. In addition, this alkyne probe can be turned on from the Raman-silent state both on a model liposomal membrane and on living mammalian cells by chemical stimulus. Furthermore, in live-cell Raman imaging, activation of the present probe was observed to be accelerated under a mildly acidic condition due to low pH-enhanced conversion to the alkyne moiety 22 . These studies demonstrate the proof of principle that the present activatable probe may visualize the dynamics and trafficking of cholesterol-rich microdomains from the activation point of location particularly in a mildly acidic environment on living systems.

Results
Design and synthesis of alkyne-labeled steroid probe. We first designed an alkyne-labeled steroid probe for enabling activation of the precursor in a lipid bilayer membrane. When steroids are inserted into a lipid bilayer membrane, ring D, tethering an alkyl chain, locates deep inside the membrane; accordingly, ring A lies toward the membrane surface. Thus, when considering conversion from the precursor that could be used after insertion in the membrane, ring A, which is located close to the membrane surface, would be a candidate structure because it would be an easily accessible reaction point on the steroid precursor molecule. Therefore, we designed alkynyl probe 1, in which ring A of steroid is replaced with an alkynyl group (Fig. 1a). The probe 1 was synthesized efficiently through epoxidation and subsequent Eschenmoser-Tanabe fragmentation from 4-cholesten-3-one (Supplementary Scheme S1) 23,24 . Fluorescence microscopic analysis of microdomain formation on liposomes. Probe 1 was expected to form liquid-ordered domains on the membrane by preferentially accumulating on the domains. The distribution of 1 in the lipid domains of giant liposomes with a size of several micrometers was examined by using fluorescence microscopic imaging (Fig. 1b) 25 . Giant liposomes consisting of dioleoylphosphatidylcholine (DOPC), dipalmitoylphosphatidylcholine (DPPC), and 1 (40:40:20) were formed, and the liquid-ordered (raft-like) domains were stained with fluorescein-labeled Cholera toxin subunit B. The disordered membrane domain was stained fluorescently with Rhodamine DHPE. The giant liposome was also prepared by employing cholesterol instead of 1 as a positive control. For the DOPC/DPPC/1 system, confocal microscopy confirmed that the two domains were separately stained with each fluorescently labeled probe ( Fig. 1c-f). This result shows that probe 1 can form raft-like domains that are similar to those observed in cholesterol systems ( Fig. 1g-j).
Raman imaging and spectral analysis of alkyne-labeled steroid probe on lipid bilayer membranes. The alkynyl steroid probe 1 has a characteristic Raman signal that can be used for alkyne-tag Raman imaging. According to previous reports [13][14][15] , the distribution of alkynyl analogue 1 on the lipid bilayer membrane was visualized by mapping its characteristic Raman signal at 2120 cm −1 on the giant liposomes. Lipid membranes that were composed of DOPC (40%), DPPC (40%), and 1 (20%) were suspended in phosphate-buffered saline (PBS) solution with a small amount of biotin-modified poly(ethylene glycol)-lipid (0.20%). The biotinylated giant liposomes, which were immobilized on a streptavidin-coated quartz substrate on the dishes through biotin-streptavidin interaction 26 , were observed with a Raman microscope. The alkyne signal induced from 1 at 2120 cm −1 was clearly mapped to the liposome membrane and overlapped well with the signal corresponding to the C− C stretching vibration of lipid components at 1450 cm −1 (Fig. 1k). Furthermore, in the Raman images, there were a few domains where both the C≡ C and C− C signals were relatively large. The Raman spectra obtained at a selected point on the domain exhibited large Raman signals arising from both C≡ C and C− C stretching vibrations (Fig. 1l).
Steroid probe 1 exhibited different behavior in living cells to that observed in the liposome experiments. Cholesterol-rich domains in living cells are known to be internalized from plasma membranes into the endoplasmic reticulum in the endocytic pathways 1 . The distribution of 1 in living HeLa cells was investigated by employing stimulated Raman scattering (SRS) after cell uptake of 1 by using methyl β -cyclodextrin (Mβ CD) 12   Conversion to alkyne-tagged steroid probe in aqueous-based media. Next, the alkyne moiety of 1 was examined to be generated under physiological conditions. As described above, it was designed to be produced through Eschenmoser-Tanabe fragmentation, which was used in the second step for the preparation of 1. In the fragmentation reaction, an aliphatic ring containing an α ,β -epoxy ketone moiety is opened by addition of p-toluenesulfonyl hydrazine (TsNHNH 2 ), resulting in the production of an alkyne group. In our case, 4,5-epoxycholestan-3-one (2), was converted in ethanol into ring-A-opened alkyne 1 (Supplementary Scheme S1) 24 . Developing this approach further, we confirmed that this reaction also proceeds very well in aqueous-based media at room temperature ( Fig. 2a). Alkyne-precursor 2 was mixed with TsNHNH 2 in a mixture of deuterated water and deuterated dimethyl sulfoxide (40% conc.) and the solution was incubated for various periods of time at room temperature. 1 H NMR spectroscopic analysis revealed that as the specific chemical shifts of 1 appeared, shifts corresponding to 2 simultaneously decreased ( Fig. 2b-f). The peak intensity of the epoxy ketone (2.97 and 3.02 ppm of diastereomers) decreased to 60% within 5 h (Fig. 2g). The IR spectrum revealed a signal at 2118 cm −1 corresponding to the formation of the alkyne moiety after incubation of the present aqueous-based system for 4 h (Supplementary Figure S4).
Alkyne formation occurs efficiently even on a lipid bilayer membrane. We prepared liposomes including 2 and investigated the alkyne formation reaction on the liposomes and subsequent labeling with an azide-substituted fluorescein through copper-catalyzed click chemistry (Huisgen cycloaddition) 27 . Only after treatment with TsNHNH 2 , emission from the tethering fluorescein was clearly detected on the pellet of liposomes after centrifugation (Supplementary Figure S5a Chemical activation of the Raman signal of alkyne-tagged steroid probe. The Raman signals of 1 formed by chemical conversion of 2 on membrane were also observed by using the giant liposomes as described above. The giant liposomes consisting of DOPC (40%), DPPC (40%), and 2 (20%) were immobilized onto the substrate of the dishes, and were observed with a Raman microscope before and after treatment with TsNHNH 2 for 40 min. After treatment with TsNHNH 2 , a sharp signal appeared at 2120 cm −1 in the Raman spectra of the whole image including the liposome, which were obtained by multicomponent analysis (Supplementary Figure S6). In contrast, before TsNHNH 2 treatment, no signal was observed at the same wavenumber in the major components separated by multicomponent analysis. Thus, the Raman signal contributed by the chemical conversion of 2 into 1 was confirmed to be detected at 2120 cm −1 . Furthermore, to visualize activation of the alkyne signal on the lipid bilayer membranes alone, the Raman signals at 2120 cm −1 were mapped before and after treatment with TsNHNH 2 . Before treatment with TsNHNH 2 , only noise was observed in the image recorded at the alkyne stretching frequency, whereas the C− C signal at 1400 cm −1 was correctly mapped to the liposome membrane ( Fig. 3a-d). After treatment with TsNHNH 2 , the alkyne signal was clearly mapped to the liposome membrane and overlapped perfectly with the C− C signal (Fig. 3e-h). In the Raman spectrum obtained at a selected point on the membrane, peaks due to both C≡ C and C− C stretching vibrations were confirmed, in contrast to the spectrum obtained before treatment with TsNHNH 2 (cf. Fig. 3i and j).
Finally, alkyne formation was also observed on living cells by SRS microscopy. The precursor 2 was incorporated into the cellular membranes of HeLa cells in almost the same way as alkyne steroid probe 1. After treatment with TsNHNH 2 for 30 min, SRS images at the wavenumbers of 2115 cm −1 and 2930 cm −1 were obtained for visualizing the distributions of the activated alkyne probe. In the image at 2930 cm −1 , the whole cell region can be simply visualized by mapping the signal of C-H bonds in lipids and proteins 28 . On the image, the granular signals on which the SRS spectra have the clear peak at 2115 cm −1 were observed from the intracellular region when the treatment with TsNHNH 2 was performed under a mildly acidic condition (Fig. 4a-c and g). By contrast, no clear signal was observed from the cell treated with TsNHNH 2 at a neutral pH in the image at 2115 cm −1 (Fig. 4d-f and h). Thus, the activation of the C≡ C stretching vibration signal was confirmed in live-cell imaging, and under the present experimental condition, the activated signals were selectively observed from the cells in a mildly acidic medium. Here, the influences of the probe and TsNHNH 2 on cell viability were investigated with a commercially-available viability assay kit, Cell counting kit-8 (CCK-8) assay. We confirmed that incorporation of the precursor steroid analogue 2 into cellular membranes with Mβ CD had no influence on the viability, and incubation with TsNHNH 2 for 30 min briefly decreased the viability to approximately 80% (Supplementary Figure S8).

Discussion
In this study, a ring-A-opened alkyne steroid analogue probe has been synthesized as a first chemically-activatable Raman probe. From the pioneer studies of Sodeoka's group 13,14 , alkyne-tag Raman imaging is a powerful approach for visualizing small biomolecules in living systems for a long period without perturbing their intrinsic properties. If the alkyne group tagged on the molecules could be generated by biorthogonal stimuli under physiological conditions, the alkyne-tagged probe would be more valuable as an activatable Raman probe for stimuli-responsive selective visualization. However, as evidenced by the fact that compounds containing C≡ C bonds exist very rarely in cells, it is difficult to obtain a C≡ C bond from its precursor under physiological conditions through the use of external stimuli. In looking for a suitable reaction that could be used to generate the alkyne moiety, we focused on reactions that proceed well under mild conditions. We focused in particular on Eschenmoser-Tanabe fragmentation 23,24 , where an alkyne group is converted from a stable epoxy ketone moiety by addition of TsNHNH 2 . In the previous reports, this reaction proceeded in polar solvents such as alcohols at slightly high temperatures. Furthermore, TsNHNH 2 is water soluble, and to our knowledge, the severe cytotoxicity of TsNHNH 2 has not been reported. Accordingly, it seemed to be promising that the fragmentation reaction was employed as the alkyne generation reaction in living systems. Thus, we decided to employ the oxidized cholesterol 2 containing an α ,β -epoxy ketone moiety, which can be converted to the alkyne steroid probe 1 through the fragmentation reaction.
The alkyne steroid probe 1 was demonstrated to form microdomains on lipid bilayer membranes. Formation of the liquid-ordered microdomains which enrich cholesterol and saturated phospholipid has been reported to occur spontaneously in giant liposomes containing cholesterol, saturated phospholipid, and unsaturated phospholipid 29 . The microdomains are widely considered to be analogous to lipid raft in cell membranes. In the model membranes, the formation of the raft-like domains is highly dependent on cholesterol composition because the driving force of the lateral segregation is the difference in the affinity of cholesterol for the two phospholipids: the molecular flatness of the rigid sterol ring favors interaction with straighter and stiffer hydrocarbon chains of saturated lipids and disfavors interaction with the more bulky unsaturated ones 2 . In this study, the liquid-ordered and disordered domains were separately stained by membrane domain-specific fluorescent probes in giant liposomes containing DOPC (40%), DPPC (40%), and 1 (20%) (Fig. 1). When the content rate of 1 was decreased to 13%, the domain separation was not observed (Supplementary Figure S1). This result strongly suggested that the ring-A-opened steroid structure of 1 contributed to the domain formation in the similar manner as cholesterol. Furthermore, in the Raman image of the liposome, there are the domains where both the C≡ C and C− C signals were relatively large (Fig. 1k). This is probably because the large signals were derived from the accumulated 1 and highly-packed DPPC in the liquid-ordered domains. From these results, the alkyne steroid probe 1 was suggested to behave as the analogue of cholesterol in the formation of microdomains. Similar to cholesterol and 1, the precursor steroid analogue 2 was also confirmed to lead to the microdomain formation (Supplementary Figure S2). From the results, the present activatable probe was suggested not to drastically alter their distribution to the microdomains before and after activation.
Conversion of the precursor steroid analogue 2 into the alkyne analogue 1 was demonstrated in aqueous-based media and on liposomes. The precursor 2 has low solubility in water because of its hydrophobicity. Accordingly, the conversion reaction with TsNHNH 2 was monitored by 1 H-NMR in a mixture of deuterated water and deuterated DMSO (40%). As a result, the reaction was confirmed to proceed at room temperature (Supplementary Figure S4), although it seemed to stop at about 40% conversion (Fig. 2). This is probably because the precursor 2 was not completely dissolved into the reaction mixture solution. Actually, in our further trial using a different water-soluble derivative, most of the α ,β -epoxy ketone moiety was converted to the alkyne product in an aqueous solution within 1 h, and in particular, the conversion was almost finished within 15 min in a mildly acidic aqueous solution 22 . In Fig. 2g, a part of precursor 2 also seemed to be rapidly converted to 1 at the beginning of the reaction within a few hours because the dissolved fraction of 2 could quickly react with TsNHNH 2 . Thus, alkyne formation through the ring-opening reaction of epoxy ketone proceeded in the aqueous-based media efficiently in a short time as long as the precursor moiety was homogenously diffused. The same conversion was investigated on the lipid bilayer membranes for showing the possibility of its application to cellular membrane imaging. Alkyne formation on the membrane was preliminarily detected by fluorescence labelling through click reaction with an azide-modified dye. Selective fluorescent staining of the liposomal membrane including the precursor 2 was observed after treatment with TsNHNH 2 (Supplementary Figure S5). This result indirectly suggested the progress of the alkyne formation reaction on the membranes. Therefore, to directly indicate the alkyne formation, the liposomal membrane including 2 was observed by Raman microscopy before and after TsNHNH 2 treatment (Fig. 3). The Raman signals derived from the alkyne moiety at 2120 cm −1 was clearly mapped to the membrane in the image obtained after TsNHNH 2 treatment (Fig. 3g), whereas only noise was observed in the image at 2120 cm −1 before TsNHNH 2 treatment (Fig. 3c). This result directly shows that precursor 2 reacted on the liposome membrane in the presence of TsNHNH 2 to form alkyne 1.
The activatable steroid probe was applied to live-cell Raman imaging of cholesterol-rich domains. According to previous reports 12 , the steroid analogue probes were introduced onto the plasma membranes through Mβ CD-mediated replacement of the inherent cholesterol with the probes. In the preliminary experiment, we introduced alkyne steroid probe 1 into HeLa cells and observed the intracellular distribution of the alkyne signal by spontaneous Raman microscopy. However, the alkyne signal was only weakly detected from the cell even by increasing the cumulated number of scanning. To obtain the image with both higher sensitivity and higher speed, the cells were observed with the SRS imaging system 28 . As a result, alkyne-derived granular signals were clearly observed from the intracellular cytosolic region in the image at 2115 cm −1 (Supplementary Figure S3). Cholesterol-rich raft domain is well known to internalize from the plasma membrane to the intracellular domain via the endocytosis pathway 1 . After steady-state distribution in living cells within 30 min, the signal of cholesterol analogue probes was often observed from plasma membrane (PM), endocytic recycling compartment (ERC) and lipid droplets 30 : the signals on PMs appeared as a diffuse, hazy one; those on ERCs and lipid droplets were bright granular spots and larger punctate structures, respectively. In our case, only the signals of the alkyne steroid probe on granular or punctate organelle were clearly detected probably due to its higher concentration than that on PMs (Supplementary Figure S2). In a previous report 16 , the alkyne signals of the similar cholesterol probe were also observed as granular spots in the cytoplasm by SRS microscopy, and it was reported to show accumulation of the probes in lipid droplets. Similarly, in the case of precursor probe 2, the granular signals with the peak at 2115 cm −1 were observed in the cytoplasm after TsNHNH 2 treatment under a mildly acidic condition (Fig. 4a-c). This result strongly suggests that some of precursor 2 was converted to alkyne steroid probe 1 through the alkyne formation reaction on cellular membranes, followed with endosomal internalization to lipid droplets. Thus, chemical activation from the Raman-silent state of the ring-A-closed precursor was possible even on living cells. It is noteworthy that the alkyne signal was not only observed from the granular spots but also from the cytosol area by optimizing the conditions of SRS imaging such as the laser intensity, although the intensity of the cytosol signal was relatively low compared to the granular signals ( Fig. 4c and g, area 2). Furthermore, the alkyne signal in the cytosol disappeared after incubation in a culture medium for 2 h, suggesting that the cholersterol-rich domains on intracellular traffic were localized to granular lipid droplets (Supplementary Figure S7). These results suggest that the distribution change of the present steroid probe can be visualized after chemical activation. By contrast, such activation of alkyne signals was not observed after TsNHNH 2 treatment at pH7.4 (Fig. 4d-f). In our other work, the rate of alkyne formation was shown to be almost five times slower under neutral conditions than under mildly acidic ones 22 . Accordingly, it is assumed that the conversion rate at pH7.4 was too low to give the visible alkyne signals in the SRS images under the present experimental condition. This result strongly suggests that the present activatable steroid probe can be applied to selective visualization of the cholesterol-rich domains which are treated with TsNHNH 2 in a mildly acidic environment in living systems. It is well-known that a decrease in pH can occur locally in specific intracellular organelles such as endosomes and lysosomes or in certain pathological regions, cancer tissues and inflammatory sites 31,32 . Therefore, low-pH-activatable imaging probes have been studied in the fields of bioanalytical and biomedicinal chemistry 18,33 , and actually, were applied to selectively visualize low-pH lung tumors in vivo to assist in surgical planning and operations 18 . The present low-pH-activatable steroid probe is also potentially applicable to live-cell or in vivo Raman imaging of the trafficking and distribution of cholesterol-rich domains under/from acidic environments in living systems. In addition, it was shown from the results of the cell viability assay that the present probes and the activation procedures do not have large impact on the cell viability (Supplementary Figure S6).
The present probe is the first activatable Raman probe for the cholesterol-rich domains in cellular membranes, and potentially applicable to visualization of their dynamic localization through chemical pulse activation. Chemical activation generally depends on the environmental factors such as temperature and pH, and the local conditions such as the reactant concentration, permeability and diffusion. Accordingly, the membrane domains which exist in a specified environment in living systems may be selectively visualized, and the domains only in a limited area are potentially visualized by localizing the reactant hydrazine through its modification with a targeting moiety or a photo-removable protection group. The present alkyne steroid probe thus will be a platform tool to take a big step toward producing some activatable imaging methods for understanding the intracellular distribution pathways and the environment-specific dynamics of the cholesterol-rich raft-like domains.

Methods
Synthesis of probes. Precursor steroid probe 2 was synthesized through epoxidation of 4-cholesten-3-one (from Wako Chemicals, Osaka, Japan), and alkynyl steroid probe 1 was synthesized from 2 through Eschenmoser-Tanabe fragmentation reaction 24 . The details of the synthec procedures were shown in Supplementary Information.

Domain formation on giant liposomes.
Fluorescently-stained raft-exhibiting giant liposomes were prepared essentially according to a previous report 25 . The films of the lipid mixture (DOPC/DPPC/cholesterol or 1/ D -(+ )-glucose = 2:2:1:15) with 0.2% Rhodamin-DHPE and 1% GM 1 was prepared in glass tubes, and hydrated with deionized water, followed with incubation at 37 °C for one hour to form giant vesicles. The liposome suspension was combined with a solution of fluorescein-labeled CtxB-488 for staining the cholesterol-rich raft-like domain. The fluorescent images of liposomes were acquired using a confocal fluorescent microscope (LSM 510 META confocal microscopy, Carl Zeiss, Jena, Germany).
Evaluation of the chemical activation with 1 H-NMR spectroscopy. 1 H-NMR spectra of the precursor 2 were measured after incubation with TsNHNH 2 in aqueous-based media for various periods. To a solution of 2 in DMSO-d 6 (0.4 mL) was added a solution of TsNHNH 2 (20 mM) in D 2 O (0.6 mL). After mechanically shaking for several hours at room temperature (Micro Mixer E-36, from TaiTec, Tokyo, Japan), the solution was extracted with chloroform-d (0.6 mL). The 1 H-NMR spectra were measured with Avance 600 (600 MHz, from Bruker, Germany).

Raman imaging of probe-introduced giant liposomes.
Raft-exhibiting giant liposomes consisting of the lipid mixture (DOPC/DPPC/1 or 2/D-(+ )-glucose = 2:2:1:15) with 0.2% biotin-modified poly(ethylene glycol)-lipid were prepared as described above. The biotinylated giant liposomes were immobilized on a streptavidin-coated quartz substrate on the dishes through biotin-streptavidin interaction 26 . In the case of 2, a solution of TsNHNH 2 in MilliQ (20 mM) was added onto the dishes for chemical conversion of 2 to 1 on the liposome. After treatment with TsNHNH 2 for 40 min, the TsNHNH 2 solution was removed, and the surface was rinsed with MilliQ water. The bright field images and Raman images were acquired with a confocal unit Renishaw inVia Raman Microscope (Renishaw plc, UK). The acquired images were analyzed and processed for multicomponent analysis with the software WiRE 4.1 (Renishaw plc, UK).

SRS imaging of probe-introduced living cells.
Either the synthesized alkynyl steroid probe 1 or the precursor probe 2 was introduced into HeLa cells. The cultured cells were treated with the mixed solution of 1 (40 mM) or 2 (10 mM) with Mβ CD (100 or 150 mM) in PBS (100 μ L) for 2 min. After treatment, the culture dish surfaces were rinsed with PBS twice. In the case of 2, the cells were treated with DMEM including 20 mM TsNHNH 2 for 30 min to activate the alkyne signal of the probe. The pHs of the TsNHNH 2 solutions were adjusted to pH6.4 and 7.4. The images of the cells were obtained with the SRS imaging system, which was previously reported 28 .