UvA-DARE ( Digital Academic Repository ) Targeting pathogen metabolism without collateral damage to the host Haanstra

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(A and B): Two independent batches of erythrocytes (black or white symbols) were washed in HEPES buffer, inoculated in in trypanosome cultivation medium (HMI-9) and followed for 5 or 7 days. (A) Cell densities of erythrocytes. Each data-point represents an averages of two counts (B) Metabolite concentrations in samples from the cultures as determined by HPLC as described previously 1 . Metabolites were separated on a Shimadzu HPLC with a Phenomenex column (Rezex ROA Organic Acid) and glucose was detected by a refractive index detector (RID-10A) and pyruvate and lactate by a UV detector (Spd-10A). Circles: glucose, triangles: lactate; squares: pyruvate. (C) Lactate production was followed by HPLC (see panel B) over 46h in monocultures of erythrocytes (2-4x10 8 cells/ml) in HEPES-based buffer supplemented with 10 mM glucose (Glc) or 5 mM Glc and 5 mM pyruvate (Pyr). In the presence of pyruvate, the pyruvate consumption flux was 0.050±0.001 nmol/min/(10 8 cells)). Error bar shows standard deviation of two independent cultures. (D) T. brucei was cultured in the presence of L-lactic acid (starting density 3x10 5 trypanosomes/ml). Cell density was determined 4 times over a period of 27 hours and specific growth rate was calculated. Error bars show the error bars of two independent incubations. (E and F) Metabolite concentrations in cocultures that were not treated at all, or were treated with ~0.1% ethanol (final concentration). Ratio of trypanosomes:erythrocytes is 1:10 3 . (G) Co-cultures of T. brucei (start density 1-5x10 5 cells/ml) and erythrocytes (density in graph) were counted 4-5 times over a period of 24-28 hours. The specific growth rate of T. brucei was calculated from these time-points as described previously 2  shows the % of stumpy cells in the population (these being the parasite's transmission stage, generated at peak parasitaemia) and C. shows the weight changes of the mice throughout the experiment. The mice were injected intraperitoneally (with either DMSO control or phloretin) when trypanosomes were first detected in the blood sample (3rd day of infection). The first injection was followed by additional intraperitoneal injections on day 5, 7, 10 and 12. Results for all phloretin-treated mice were similar; here we show a representative result from the control group and the group treated with 10 mg/kg phloretin. D. Graph shows the parasitaemia through the course of infection with a parasite line (T. brucei AnTat1.1) able to generate chronic infections in vivo, E. shows the % of stumpy cells in the population (these being the parasite's transmission stage, generated at peak parasitaemia) and F. shows the weight changes of the mice throughout the experiment. Mice were injected intravenously (with either DMSO control or phloretin), when trypanosomes were first detected in the blood sample. (4th day of infection). This first injection was not followed by additional intravenous or intraperitonial injections. Results show the control mouse and the mouse injected with 10 mg/kg phloretin. The mouse injected with 50 mg/kg had to be sacrificed due to adverse side-effects.

Supplementary Figure S6. Related to Figure 6
We used the hepatocyte glycolysis model from 4 6 . The equilibrium constant was included explicitly in each rate equation, so that the inhibition kinetics could not shift the equilibrium. *** Glucose transport occurs via facilitated diffusion and hence the equation for the glucose transporter is different from the classical Michaelis-Menten equations. We have previously derived how a competitive inhibitor should affect the transport step (see supplement to 7 ): a competitive inhibitor would simultaneously affect the K m and the α. The extent to which an inhibitor affects these parameters depends on the ratio of two inhibition constants K i1 and K i2 . The ratio K i2 /K i1 can theoretically range between 0.5 and infinity. The simulations here consider the case that this ratio is infinity.

* For T. brucei only the glycosomal hexokinase was considered in this analysis
Ratios were calculated before numbers were rounded off.

Supplementary Table S3: Related to Figure 5 and S5
Phloretin concentrations in blood samples were determined with a HPLC-based method described in 8 .

Simulation of inhibition kinetics
Competitive inhibition was modelled by multiplying the relevant K m values by the factor (1 + [I]/K i ) 3 .
The K m of the substrate as well as that of the corresponding product was modified by the same factor, since substrate, product and inhibitor all compete for the active site. The only exception is when the product inhibits through binding to an allosteric site, such as allosteric inhibition of erythrocyte HXK by Glc6P. In the latter case, only the K m for the substrate was modified, while the K m for the product was unaffected by the inhibitor. In all cases the equilibrium constant was unaffected by the inhibitors. Table S1 lists the K m values that were changed in each particular case.

Calculation of coefficients
The flux control coefficient of an enzyme i over a steady-state flux J is defined as: in which v i is the rate of enzyme i and p is any parameter that only affects v i 9 . Since in each of the reactions considered v i was proportional to the V max of the enzyme, the V max was the parameter of choice. Flux control coefficients were calculated via the metabolic control analysis function in COPASI. We checked that all flux control coefficients over the ATP utilization flux added up to 1.
The partially normalized elasticity coefficient towards the [I]/K i ratio represents the direct effect of the inhibitor on the enzyme rate v i and was calculated at zero [I] according to the definition: The asterisk indicates that this elasticity coefficient deviates from the standard definition 9 , which would normalize for the actual concentration of the inhibitor. This modified elasticity coefficient is independent of the actual value of the inhibition constant and also exists in the limit [I]→0.
The partially normalized elasticity coefficient of an enzyme i towards its inhibitor ( * / ) was calculated as follows: The derivative of the enzyme rate v i with respect to [I]/K i was determined symbolically using Mathematica 9 software and the outcome was calculated at [I]/K i = 0 and at the steady-state metabolite concentrations in the absence of inhibitor. The resulting value was divided by the steady-state enzyme rate to obtain * / .
More on the Measurements of Oxygen Consumption Rate (OCR) and Extracellular acidification rate (ECAR) HT-22 cells were seeded in XF 96-well cell culture microplates (Seahorse Bioscience) at a density of 10,000 cells/well in 4.5 g/liter of standard culture medium and incubated at 37°C and 5% CO2 for ∼24 hours. Before starting the measurements, the growth medium was washed away and replaced with ∼180 μl of assay medium (with 4.5 g/liter of glucose (25 mM) as the sugar source, 2 mM glutamine, 1 mM pyruvate, pH 7.35), and cells were incubated at 37°C for at least 60 minutes. Three baseline measurements were recorded before the addition of compounds. Phloretin, cytochalasin B and ethanol-vehicle control were added in Port A at different concentrations and OCR/ECAR values were continuously monitored for 4 hours. To assess mitochondrial function further, respiratory chain inhibitors were used. The ATP synthase inhibitor oligomycin was injected through port B at a final concentration of 3 µM to measure OCR in the absence of oxidative phosphorylation. The protonophore carbonylcyanide-4-(trifluormethoxy)-phenylhydrazone was subsequently injected in port C at a concentration of 0.4 µM to dissipate the proton gradient across the inner mitochondrial membrane and thereby to assess mitochondrial respiratory capacity (MRC). Co-injection of the complex I/III inhibitors rotenone/antimycin A together with 2-DG in port D at concentrations of 1 µM and 500 µM respectively was used to completely inhibit O2 consumption and glycolysis. Three measurements were performed after the addition of each compound by a 4-minute mix cycle used to oxygenate the medium and a 3-minute measurement cycle to assess respiration. For comparing OCR after compound exposure to OCR in non-treated cells, the absolute cell number is insignificant as a result of the same population of cells being compared. Therefore, results are indicated as normalized OCR (% baseline rate) for each individual cell population to minimize variability resulting from slight differences in plating and viability during culture and treatment time (∼5 hours).

Enrichment of erythrocytes containing plasmodium falciparum in trophozoite stage
Trophozoite-stage packed erythrocytes (pRBCs) were enriched using a density gradient separation protocol using layers of 60% and 85% Percoll in RPMI, glucose and HEPES, pH 7.2. 10 . Alanine (3%, final concentration) was added to the mixture of each layer. On top of the Percoll layers, 200-250 uL of packed pRBC's (collected at 750xg, 3 min) was added and centrifuged immediately (9600xg, 20 min; zero deceleration). The layer between the 85% and 60% Percoll layers, which contained the enriched trophozoite-stage pRBCs was washed with pre-warmed (37°C) 5x phosphate buffered saline (PBS) and spun (600xg, 3 min). The cells were resuspended and incubated for 30 min at 37°C to allow the remaining alanine to equilibrate. The suspension was spun again (600xg, 5 min) to produce a pellet of infected cells that was used for further experiments.

Glycolytic flux measurements and inhibition for plasmodium-infected erythrocytes
The metabolic activity of infected-RBCs was determined by incubating enriched (near 100% infected), intact pRBCs in RPMI-1640 and HEPES (50 mM) buffer with 5 mM glucose and measuring enzymatically the lactate produced over 1 hr (5 time points) in absence and presence of cytochalasin B (dissolved in DMSO, final concentration < 0.5% m/v; control received only DMSO). Cell numbers were determined in a Neubauer haemocytometer and exclusion of 0.04% v/v trypan blue confirmed cell integrity. Time point samples were collected and centrifuged (800xg, 1 min) and the supernatant kept for the enzymatic determination of extracellular lactate using an LDH and NAD + linked assay. Standards and samples were incubated in a HEPES assay buffer (150 mM HEPES; pH 7.6) containing L-LDH (11 U/mL), NAD + (1.6 mM), hydrazine (16 µL/mL) and incubated for 90 mins at 21 °C. Absorbance was measured at 340 nm (Varioscan microplate reader Thermo Electron Corporation Waltham, Massachusetts, USA).

Animals and pleoiomorphic trypanosomes
Animal experiments were carried out according to the UK Animals (Scientific) Procedures act under a licence (60/4373) issued by the UK Home Office and approved by the University of Edinburgh Local ethical committee. As this was a pilot study, the sample size was small and there was no randomisation or blinding done T. brucei AnTat1.1 (approximately 5x10 5 parasites) were infected into age-matched MF1 female mice. Parasitaemia was monitored by microscopy of bloodsmears every day through the 14 days of the experiment by counting trypanosomes and extrapolating to the actual cells/ml 11 . This pleomorphic strain is as sensitive to phloretin as the Lister 427 strain that we used in the in vitro experiments 12 .
Phloretin has in the past been used to treat HepG2 adenocarcinomas in mice 13 with no reported side effects, making it the compound of choice for in vivo testing.
In the experiments a fresh batch of phloretin was used (Sigma-Aldrich), after the killing effect of the drug was established on AnTat1.1s in culture.

Intraperitonial phloretin injections
Three mice groups were set up, two mice in each group.
The mice in these three groups were injected intraperitoneally (with either DMSO control or phloretin) when trypanosomes were first detected in the blood sample (3rd day of infection). The first injection was followed by additional intraperitoneal injections on day 5, 7, 10 and 12. The mice were injected intraperitoneally with 200 µl solution with either 20% DMSO (group 1), a phloretin dose in 10% DMSO that would lead to administration of 10 mg/kg phloretin (group 2), or a phloretin dose in 20% DMSO that would lead to administration of 25 mg/kg phloretin (goup 3)

Intravenous phloretin injections
Three mice were injected intravenously (with either DMSO control or phloretin), when trypanosomes were first detected in the blood sample (4th day of infection). This first injection was not followed by additional intravenous or intraperitonial injections. The mice were injected intraperitoneally with 200 µl solution with either 20% DMSO, with a phloretin dose in 20% DMSO that would lead to administration of 10 mg/kg or 50 mg/kg phloretin. A blood sample was taken from each mouse 1 and 24h post-injection.
Considering an average mouse weight of 25 gram, a total blood volume for a mouse of 1.5 mL (https://www.nc3rs.org.uk/mouse-decision-tree-blood-sampling) and the molecular weight of phloretin of 274 gram/mole, a 10 mg/kg injection of phloretin would amount to a 600 µM concentration if it remains in the blood and a 36 µM concentration if the phloretin would get dispersed equally over the entire mouse.