(A) Expression patterns of 20-hydroxyecdysone-dependent nuclear receptors during the last nymphal instar of B. germanica, analyzed by semi-quantitative RT-PCR. BgActin5C mRNA levels were used as a reference. The blot is representative of three replicates. 20-hydroxyecdysone levels (upper part) are redrawn from ref. 18. (B) Expression pattern of BgFTZ-F1, relative to BgActin5C levels, in the CA of B. germanica females during the penultimate and last nymphal instars and the adult stage, measured by qRT-PCR.