Figure 3: BgSvp-B is the isoform necessary for adult reproduction in B. germanica. | Scientific Reports

Figure 3: BgSvp-B is the isoform necessary for adult reproduction in B. germanica.

From: Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1

Figure 3

(AC) Effect of BgSvp isoform-specific RNAi. Newly emerged sixth instar female nymphs were injected with 3 μg of dsMock (Control), dsBgSvp-A (BgSvp-Ai) or dsBgSvp-B (BgSvp-Bi). (A) mRNA levels of BgSvp-A and BgSvp-B, relative to BgActin5C levels, in the CA of 6–day-old N6 specimens, measured by qRT-PCR. (B) DAPI-stained ovarioles from 5-day-old Control, BgSvp-Ai and BgSvp-Bi adult females. Arrowheads indicate basal oocytes. (C) BgVg mRNA levels in the fat body of 5-day-old Control, BgSvp-Ai, and BgSvp-Bi females, relative to BgActin5C mRNA levels, measured by qRT-PCR. Error bars in (A and C) represent SEM (n = 5); asterisks in (A and C) indicate differences statistically significant with respect to Controls (***p ≤ 0.0001 (Student’s t test)). Scale bar in (B): 200 μm.

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