(A) Effect of RNAi of BgSvp. Newly emerged sixth instar female nymphs were injected with 3 μg of dsMock (Control) or dsBgSvp-1 (BgSvpi). Dorsal views of Control and BgSvpi animals showing normal winged adult appearance. (B) DAPI-stained ovarioles from 5-day-old Control and BgSvpi adult females. Arrowheads indicate basal oocytes. (C) Basal oocyte length of Control and BgSvpi adult females of the indicated age. Results are expressed as the mean ± S.E. (n = 25–31). (D) BgVg mRNA levels in the fat body of 5-day-old Control and BgSvpi females, relative to BgActin5C mRNA levels, measured by qRT-PCR. Error bars represent SEM (n = 4). Asterisks in (C and D) indicate differences statistically significant as follows: ***p ≤ 0.0001 (Student’s t test). (E) SDS–PAGE of hemolymph (left) and ovaries (right) from 5-day-old Control and BgSvp-1i adult females. Arrowheads indicate BgVg subunits. Scale bars: 1 mm in (A); 200 μm in (B).