(A) Expression levels of BgSvp-A, and BgSvp-B relative to BgActin5C in different tissues of 8-old-day B. germanica last instar nymph females, measured by qRT-PCR. corpora allata (CA), ovary (Ov), fat body (FB), brain (B), accesory glands (AG), prothoracic gland (PG), and gut (G). Fold changes are relative to the expression of each gene in the CA, arbitrarily set to 1. (B) Expression patterns of BgSvp-A and BgSvp-B mRNAs in the CA during the last two nymphal instars of B. germanica, analyzed by semi-quantitative RT-PCR. BgActin5C mRNA levels were used as a reference. The blot is representative of three replicates. Circulating JH levels (upper part) are redrawn from ref. 19.