Figure 4 : ILYIG forms an SDS-sensitive early prepore that remains membrane-bound.

From: Disentangling the roles of cholesterol and CD59 in intermedilysin pore formation

Figure 4

(a) ILYIG was incubated with cytoCD59-decorated, cholesterol-containing liposomes and subjected to flotation through ficoll. Toxin within gradient fractions was analyzed by SDS-AGE and western blot. Fractions span two gels with direction of fractionation indicated by Top and Bottom. Molecular weight markers are in the first lane of each gel. (b) Cryo-electron micrograph of the sample analyzed in panel a showing SDS-sensitive oligomeric prepores on liposomes (white arrows). Scale bar, 50 nm.