Involvement of extracellular TNF-α in the methylmercury toxicity of mouse neural stem cells.
(A) Effect of TNF-α antagonist on the sensitivity of C17.2 cells to methylmercury. C17.2 cells (1 × 104 or 5 × 103 cells/well) were plated into 96-well plates, and cultured in 100 μL aliquots of the medium for 24 hr. WP9QY, a TNF-α antagonist, was then added to the cells, and 1 hr later exposed to MeHg at the indicated concentrations for 24 hr. Cell survival was determined using an Alamar blue assay. Data represent the mean ± S.D. of three replicates. (B) Effect of recombinant TNF-α on cell survival. C17.2 cells (3 × 103 cells/well) were plated into 96-well plates, and cultured in 100 μL aliquots of the medium for 24 hr. Recombinant TNF-α was then added to the cells, which were cultured for another 72 hr. For further details, see the legend to Fig. 5A.