Nitric oxide (NO) regulates plant growth and development as well as responses to stress that enhanced its endogenous production. Arabidopsis plants exposed to a pulse of exogenous NO gas were used for untargeted global metabolomic analyses thus allowing the identification of metabolic processes affected by NO. At early time points after treatment, NO scavenged superoxide anion and induced the nitration and the S-nitrosylation of proteins. These events preceded an extensive though transient metabolic reprogramming at 6 h after NO treatment, which included enhanced levels of polyamines, lipid catabolism and accumulation of phospholipids, chlorophyll breakdown, protein and nucleic acid turnover and increased content of sugars. Accordingly, lipid-related structures such as root cell membranes and leaf cuticle altered their permeability upon NO treatment. Besides, NO-treated plants displayed degradation of starch granules, which is consistent with the increased sugar content observed in the metabolomic survey. The metabolic profile was restored to baseline levels at 24 h post-treatment, thus pointing up the plasticity of plant metabolism in response to nitroxidative stress conditions.
Nitric oxide (NO) is endogenously produced in diverse living organisms and regulates a wide array of biological processes. In plants, NO regulates developmental transitions such as seed germination1, photomorphogenesis2,3, flowering4,5), fruit ripening6 and leaf senescence7,8. NO is also a key regulatory molecule in the response of plants to environmental stress9,10. As a free radical, NO is prone to react with free radical oxygen species and also with metals11. It has been reported that NO can enhance or reduce the redox status of the plants depending on either acting in a chronic or acute mode12. NO has the potential for altering the function, activity, stability and subcellular localization of many target proteins through post-translational modifications (PTMs). NO induces PTMs such as S-nitrosylation of C and nitration of Y13 but also ubiquitylation of K and phosphorylation of S, T and Y14. PTMs alter the activity or the function and also the stability of target proteins through regulation of proteolytic degradation or subcellular re-localization15.
The major sources of NO in the atmosphere are those derived from industrial activity and car engines16 as well as the microbial-related release from soils17. Because of the spontaneous conversion of NO to NO2 under aerobic conditions, it is frequent to talk about NOx when NO is supplied in an oxygenated environment. Despite NO being released by anthropogenic or by biotic soil microorganism activities, the levels of NO have been increasing continuously in the Earth atmosphere since industrial revolution started18. In this context, plants may be exposed to relatively high NO concentrations that would potentially alter their growth and responses to the environment. On the other hand, NO is also endogenously produced in plants through both oxidative and reductive biosynthetic pathways, and its production is enhanced under stress conditions19,20. The oxidative status generated by reactive oxygen species in stressed plants seems to be alleviated by NO through the improvement of the antioxidant capacity, thus contributing to redox homeostasis21.
In contrast to the increasing knowledge of the effect of NO on protein function, our current knowledge on the NO impact on global metabolome of plants is rather scanty. Although targeted metabolic approaches have been reported in addressing the effect of oxidative stress on redox-related metabolites22, a global metabolomic approach of NO-triggered effects in plants is still pending. We employed such untargeted metabolomic approaches to identify the metabolic targets of NO in plants exposed to an acute dose, which resembles a plant response to an extreme environmental stress condition. Metabolomic data analyses allowed us to identify polyamines, phospholipids amino acid and sugars as main metabolites involved in the activation of plant responses to nitroxidative stress.
Metabolomic analyses reflects a transient reprogramming response to exogenous NO
Arabidopsis thaliana seedling exposed to a pulse of NO gas were used as model for mimicking an acute exposure of plants to a peak in environmental levels of NO or to intracellular accumulation of large amounts of NO in the production foci in plants under stressful conditions. Seedlings were exposed to a single dose of 300 ppm NO for 5 min and then, samples were harvested for untargeted metabolomic analyses comprising a total of 232 named biochemicals. Similar analyses were also performed in mock treated plants. Samples were collected at the time of the treatment (PRE), and then at 6 h and 24 h after exposure to NO or mock treatment (Fig. 1a). Plants exposed to NO underwent a transient though remarkable metabolic alteration by 6 h after treatment. A statistically significant increase in the amount of lipid, carbohydrate, amino acid and nucleotide categories of analyzed metabolites was detected in plants by 6 h after exposure to NO (Fig. 1a). By 24 h, the metabolomic status of treated plants was restored to that of untreated plants (Fig. 1a). Supplementary Table S1 includes the complete dataset with pathway heat maps and box and line plots for every analyzed metabolite showing the comparative fold- levels for every metabolite in the different samples as well as the statistical significance for the different comparisons. Only a few metabolites showed a significant difference when PRE and mock 6 h samples were compared, thus indicating only a small proportion of the metabolites analyzed were potentially regulated by circadian-related events in the experiment (Fig. 1a and Supplementary Table S1). By contrast, around 70% of the metabolites showed significant alteration in their endogenous levels by 6 h after exposure to NO (Fig. 1a and Supplementary Table S1). Significantly lower number of changes were detected in samples collected at 24 h after exposure (Fig. 1a and Supplementary Table S1), thus suggesting NO treatment has an extensive though transient metabolic effect on Arabidopsis plants. The Principal Component Analysis (PCA) confirms this general pattern. The PRE and MOCK 6 h or 24 h samples did not separate well, the NOx 24 h samples had only partial separation, and indeed the NOx 6 h samples were all clearly separated from this grouping (Fig. 1b). Moreover, to assess the compounds which meet the statistical criteria for significant differentiation between the analyzed samples, we applied two types of tests to compare the five experimental groups. The first approach was to compare each treated or mock-treated group to the PRE samples, using Welch’s Two Sample t-Test. This approach yields information on the effect of time parameter on the experiment. A summary of the numbers of biochemicals that achieved statistical significance (p ≤ 0.05), as well as those approaching significance (0.05 < p < 0.1), is shown in Table 1. One would expect approximately 10–15 compounds to reach significance by chance alone with 232 compounds tested. In these comparisons the mock samples showed only 12 or 24 compounds at 6 h and 24 h, respectively, which were significant (Table 1), thus suggesting the mock treatment had little effect on the metabolome. By contrast, the NOx treatment resulted in 133 compounds being significant at the 6 h time point, and 55 at 24 h (Table 1), consistent with a robust experimental effect. To eliminate any diurnal effects in the data, we used an ANOVA Contrast test to compare the NOx to MOCK plants at each time point individually, and to score the time-related differences within each treatment pair. Again, the results were quite consistent with the observations taken from the heat map (Fig. 1a), PCA (Fig. 1b), and t-test results (Table 1). There were 154 compounds meeting the cut-off when comparing NOx 6 h to MOCK 6 h, but only 29 when compared at the 24 h time point (Table 1 and Supplementary Table S1), consistent with the idea that plants experienced a transient and acute response to NO exposure, and then, most metabolites returned to baseline levels after 24 h.
Altered levels of amino acids and dipeptides suggests NO treatment increased protein breakdown
The elevated content of proteinogenic amino acids and dipeptides in the NOx 6 h compared to the MOCK 6 h or PRE samples (Supplementary Table S1) strongly suggest NO is inducing protein degradation. The exceptions to this pattern were amino acids that serve important metabolic functions in addition to protein synthesis. This includes glutamate, glutamine, and arginine, which are involved in nitrogen trafficking and storage, and glycine and serine, which are utilized in the photorespiration reactions, which endogenous content did not change significantly by NO treatment (Supplementary Table S1). The branched chain amino acids, leucine, isoleucine, and valine are typically at relatively low levels in cells, and function almost exclusively in protein synthesis, so an increase in their levels often indicates net protein turnover. These compounds, as well as the aromatic amino acids were more than twice the levels in NOx 6 h samples relative to MOCK 6 h (Supplementary Table S1). The aromatic amino acids are involved in other important pathways, such as the large phenylpropanoid family pathways (phenylalanine), tocopherol production (tyrosine), and hormone and glucosinolate synthesis (tryptophan), which were relatively unchanged or even dropped by NOx treatment (Supplementary Table S1). Moreover, the content of a common precursor of aromatic amino acid biosynthesis such as shikimate was lower in NO-treated relative to mock-treated plants at both 6 and 24 h time points (Supplementary Table S1), thus suggesting either the increased levels of aromatic amino acids caused a feed-back inhibition on the biosynthetic pathway or, alternatively, their increased levels are the result of active protein turnover.
We have observed that by 30 min after exposure to NO the nitroblue tetrazolium staining of superoxide anion in Arabidopsis seedlings was significantly reduced, thus suggesting NO efficiently scavenged superoxide (Fig. 2a). Peroxynitrite, a potent nitrating agent, is formed upon superoxide scavenging by NO, thus suggesting nitration of target proteins may occur in plants exposed to NO. We have analyzed the total and NO-related modified protein profile in plants after the NO pulse. No general changes were observed in the total protein pattern detected after monodimensional electrophoresis (Fig. 2b). Exogenous NO enables the modification of proteins through S-nitrosylation or nitration of cysteine and tyrosine residues, respectively13. We found that many nitrated and S-nitrosylated proteins started to accumulate by 1 h and reached maximum accumulation between 3 and 6 h after exposure of plants to NO (Fig. 2b).
Acute exposure to NO triggers the accumulation of polyamines and responses to oxidative stress
Plants exposed to NO also showed specific effects on α-ketoglutarate-derived amino acids of the glutamate family. The levels of γ-aminobutyrate (GABA) and 2-aminobutyrate were slightly increased, and the increase was higher (4.3-fold) for γ-hydroxybutyrate (GBH) by 6 h after exposure to NO (Supplementary Table S1). It has been reported that the metabolism of GABA leads to accumulation of GBH when plants are exposed to low oxygen or high light conditions, and also that under those stress conditions accumulation of GABA and alanine was also detected23, which is in agreement with our data (Supplementary Table S1). Also in the glutamate family of amino acids, the levels of proline and ornithine were 1.8- and 4-fold increased, respectively, and also their corresponding N-acetyl derivatives were 1, 7- and 5.8-fold augmented, respectively (Supplementary Table S1). The increased levels of proline and ornithine were also accompanied by significant increases in the content of polyamines such as putrescine (2.3-fold) and spermidine (1.4-fold) as well as the precursor agmatine (2.8-fold) in NO-exposed plants after 6 h (Fig. 3 and Supplementary Table S1).
Cysteine is an unique example where active synthesis of an amino acid was evident in response to NO treatment. It is formed from serine in two steps, through the O-acetylation of serine by acetyl-CoA to form O-acetylserine, followed by the incorporation of hydrogen sulfide, displacing the acetyl group24. Cysteine synthesis is often the rate limiting step for the formation of glutathione, a critical compound in cellular redox responses25. We found an increase above 2-fold in all these compounds in NOx 6 h plants. O-acetylserine and the non-enzymatically rearranged N-acetylserine were 17- and 11-fold increased, respectively (Supplementary Table S1), thus suggesting the exposure to NO triggered a strong demand for cysteine production as a mean to remediate oxidative stress. In agreement with that, perturbations of several other oxidative markers, namely increased of oxylipins 13-HODE, 9-HODE and 9,10-hydroxyoctadec-12(Z)-enoic acid produced through the LOX pathway26, were detected. Also consistent with NO-treated plants undergoing oxidative stress, the endogenous content of threonate, which is a downstream product of ascorbate metabolism27, were more than 4-fold higher in NO-treated plants by 6 h, and also a moderate increase in both oxidized and reduced glutathione content was detected (Supplementary Table S1). By contrast, the content of α-tocopherol, which together with glutathione and ascorbate constitute the triad of main antioxidants in plants undergoing oxidative stress28, were around 50% lower by 6 h after NO treatment (Supplementary Table S1). These data together suggest that NO-exposed plants respond to oxidative stress through a complex process.
Altered lipidome reflects changes in lipidic structures such as membranes and cuticle in NO-exposed plants
The content of compounds belonging to the lipid category underwent large changes in NO-treated plants. Alterations were strictly limited to the six hour time point (Fig. 4). A significant increase in the free polyunsaturated fatty acids (PUFAs) content of around 4-fold for linoleate and linolenate, and around 2-fold increase for the C20 PUFAs dihomolinoleate and dihomolinolenate was detected (Supplementary Table S1 and Fig. 4). We also found a strong increase in several lyso-lipids such as 1-palmitoyl-GPC (8.9-fold), 1-palmitoyl-GPE (13.2-fold), 1-stearoyl-GPC (5.7-fold), 1-linoleoyl-GPC (10.8-fold), 1-linoleoyl-GPE (8.9-fold), 2-linoleoyl-GPC (4.8-fold) and 2-linoleoyl-GPE (5.4-fold) (Supplementary Table S1 and Fig. 4). Altered content of free fatty acids and phospholipids are likely connected to processes of lipid trafficking in either membrane remodeling or alteration of lipidic leaf structures such as cuticles29. We tested whether cell membranes or cuticles were altered in Arabidopsis plants upon exposure to NO. Nitrooxidative stress produced by the acute pulse of NO can severely damage membranes by lipid peroxidation30, thus altering their permeability. The integrity of membranes was monitored in vivo by staining roots with propidium iodide that only stains nuclei if membranes become permeable, but it remains staining membranes and walls if cell membranes are intact. Roots of untreated plants display non altered plasma membranes as demonstrated by propidium iodide decorating the cell membrane with no permeation into the cells (Fig. 5a). By contrast, by 30 min after exposure to NO the roots of NO-treated plants displayed numerous cells with propidium iodide-stained nuclei, thus indicating the permeability of their membranes was altered (Fig. 5a). Also the formation, integrity and permeability of the leaf cuticle are highly connected to lipid metabolism in Arabidopsis31. We checked whether the permeability of the leaf cuticle was influenced by the exposure to NO. Wild type Arabidopsis plants treated with exogenous NO as well as the Arabidopsis nia1nia2noa1-2 triple mutant, depleted of endogenous NO32, were tested for cuticle permeability. Assays of cuticle permeability after application of toluidine blue or calcofluor white demonstrated an enhanced permeability of the cuticle in NO-deficient leaves that was reversed by treatment with exogenous NO (Fig. 5b).
Altered purine, pyrimidine and chlorophyll metabolism suggest NO enhanced nucleic acid turnover, chlorophyll degradation and non-programmed cell death
The metabolomic data also reflected a significant increase in metabolites involved in purine and pyrimidine metabolism. The levels of allantoin, guanine, urate, cytidine, cytosine-2′,3′-cyclic monophosphate, pseudouridine, uridine and uracil were all increased (between 1.5- and 7.6-fold) by 6 h after NO treatment (Supplementary Table S1). However, all these metabolites returned to the baseline levels by 24 h, with special emphasis on uracil, which moved from a 7.6-fold increase by 6 h to non-significantly changed levels by 24 h (Supplementary Table S1). It is noteworthy mentioning that, in turn, the levels of nucleosides such as adenosine, adenosine-2′-monophosphate and inosine are between 20 and 45% reduced by 6 h after plants were exposed to NO (Supplementary Table S1). In agreement with the enhanced metabolism of purines and pyrimidines, we found a pattern of progressive genomic DNA degradation upon exposure of plants to the NO pulse (Supplementary Fig. S1a).
On the other hand, the metabolomic data also suggest NO induces chlorophyll degradation. Pheophorbide a, which has been identified as a genuine intermediate of chlorophyll breakdown33, was 3.7-fold increased by 6 h after NO treatment (Supplementary Fig. S1b), thus suggesting Pheophorbide a oxidase (PAO) activity should be reduced in NO-exposed plants. The absence of PAO activity in different plants has been correlated with increased cell death, and pheophorbide a has been reported to be involved in both light-dependent and light-independent cell death mechanisms34,35. We checked whether treatment with exogenous NO alters the endogenous levels and leads to cell death in exposed leaves. Figure 6a shows the endogenous levels of NO after staining roots with DAF-FM diacetate. The endogenous levels slightly decreased by 2 and 4 h after exposure to exogenous NO and modest increases were observed by 6 and 24 h (Fig. 6a) but increases were far below those detected in plants treated with a known inducer of NO such as salicylic acid36 (Fig. 6b). Evans blue staining of plants at different times after NO treatment showed blue stained spots of dead cells at 6 h and 24 h after exposure to NO (Fig. 6c) and although seedlings were slightly chlorotic by 24 h plants they were fully viable showing normal growth by seven days after treatment (Fig. 6c).
Exposure to NO affected photorespiration and central carbon metabolism
By 6 h after exposure to NO, the content of the glycolisis intermediates glucose-6-phosphate, glycerate and pyruvate decreased (0.57-, 0.59- and 0.83-fold, respectively) if compared to mock-treated plants at the same time point (Fig. 7a and Supplementary Table S1). It is noteworthy mentioning that glycerate, which is an intermediate in photorespiration, kept levels significantly reduced by 24 h after exposure to NO (Supplementary Table S1). Also the levels of two metabolites of the TCA cycle such as α-ketoglutarate and malate were significantly reduced by 6 h in NO-treated plants (Fig. 7a and Supplementary Table S1). Finally, sedoheptulose-7-phosphate and ribose-5-phosphate, both intermediates of the Calvin Cycle were not different at the six hour time point, but were significantly lower (0.32-, 0.58-fold, respectively) at 24 hours (Supplementary Table S1). The content of sucrose, the downstream product of photosynthesis, although increased (2.5-fold) by 6 h was significantly reduced (0.33-fold) by 24 h (Fig. 7a and Supplementary Table S1). Also different sugars including amino and nucleotide sugars as well as disaccharides were increased (between 1.4- and 3-fold) in the 6 h time point in NO-treated plants (Supplementary Table S1). Remarkably, three of those compounds arabitol, ribulose and ribitol were strongly reduced (0.04-, 0.54- and 0.74-fold, respectively) by 24 h after NO treatment (Supplementary Table S1). In agreement with exogenous NO altering photosynthetic metabolism and triggering differential accumulation of sugars, we have detected a significant reduction in the starch granules in the hypocotyls of NO-treated seedlings by 3 h after exposure to NO (Fig. 7b).
The nature of NO as a gas and as a free radical molecule makes it highly diffusible but at the same time very reactive. NO mode of action is likely based on the existence of sharp concentration gradients with high local concentrations in the microenvironment where it is produced. In an attempt to mimic conditions of high local accumulation of NO such as those reached under natural stress-related conditions, we exposed Arabidopsis plants to a short pulse of a high dose of exogenously applied NO gas. Most of the studies quantifying NO production in plants measure NO emission, which is just a fraction of the total NO synthesized inside cells. The recent implementation of techniques using microchip electrophoresis and laser-induced fluorescence detection allowed reporting intracellular concentrations of NO around 0.6 mM for human Jurkat cells that can be increased to 1.5 mM under stress conditions induced by lipopolysaccharides37. Unfortunately, no such approach has been implemented for plant cells. Nevertheless, we have made a conservative estimation of the exogenous NO dose that would be required to approximate the endogenous content to a concentration peak closer to those ranges of concentration. A short pulse of 300 ppm NO gas would allow to raise the endogenous NO levels to values still clearly below 0.5 mM but high enough to mimic a response in the whole plant similar to that experienced locally in the production foci under stressful conditions. We then analyzed the metabolic effects that the acute exposure to NO produced in Arabidopsis plants. The metabolome of NO-treated plants changed extensively by 6 h after treatment but alterations were transient as by 24 h the metabolome was roughly similar to those of untreated or mock-treated plants. This sharp and transient response talks about the extraordinary plasticity of Arabidopsis metabolism. It has been reported that NO counteracts oxidative stress either by acting as a direct antioxidant or by functioning as a signaling molecule that alters gene expression. When acting as an antioxidant, it mainly scavenges reactive oxygen species (ROS), such as superoxide anions to form peroxynitrite, which has a large potential in nitrating target proteins38. Our data are consistent with this antioxidant activity as we detected a reduction in nitrobluetetrazolium-staining of superoxide upon NO pulse treatment (Fig. 2a) and concomitantly, treated plants accumulated nitrated proteins (Fig. 2b). Moreover, NO-treated plants showed a significant increased production of polyamines and cysteine (Supplementary Table S1), which are both good indicators of NO activating processes devoted to remediate oxidative stress. However, extensive evidence suggests that NO is somehow involved in paradoxical processes exerting sometimes opposing regulatory functions. It has been reported both antioxidant and prooxidant effects of NO12. These paradoxical roles exerted by NO might be due to multiple factors including the relative cellular concentration, the location where it is produced and the complex interacting microenvironment. As an example, the increased polyamine production has been reported to act as antioxidant but they also trigger the production of hydrogen peroxide as a result of their metabolism39. In addition, the endogenous content of two important antioxidant molecules such as ascorbate and α-tocopherol29 were reduced in NO-treated plants (Supplementary Table S1) thus suggesting NO was somehow lowering the antioxidant potential of the plant. Although ascorbate is predominantly metabolized through the reversible oxidiation to dehydroascorbate by the ascorbate–glutathione cycle, it can also escape the ascorbate–glutathione cycle by being broken down through irreversible reactions that lead to threonate40. Remarkably, a significant increase in the content of threonate was detected in NO-treated plants, thus suggesting NO is not triggering the oxidation of ascorbate but its catabolism to non antioxidant molecules.
Metabolic changes were especially relevant in the categories of peptides and amino acids, carbohydrates and lipids (Fig. 1a), thus suggesting NO is altering central processes of primary metabolism. Among these metabolic categories, changes in the lipidome have a signaling potential41 that is worth to note. The content of several phospholipids was significantly enhanced upon NO treatment (Fig. 4). The huge increases (5 to 13-fold) of lyso-lipids (Supplementary Table S1 and Fig. 4) suggested a strong activation of lipase activities at six hours. Consistently, we also saw similar patterns of increase for linoleate and linolenate (Supplementary Table S1 and Fig. 4), which are the two predominant PUFAs in Arabidopsis membranes. In turn, we did not detect palmitate, one of the main sn-1 position saturated fatty acids. We also saw somewhat elevated levels of two C20 PUFAs, dihomolinoleate (20:2n6) and dihomolinolenate (20:3n3 or 3n6). These fatty acids are reported to occur in Arabidopsis membranes in only trace amounts42. Their detection here could reflect the lipase action on trace lipids, or it could result from the enhanced elongase activity, either constitutively expressed or induced by NO action. Besides the increase in phospholipids, the NO-exposed plants had also elevated contents in the oxylipin molecules 13-HODE + 9-HODE and 9,10-hydroxyactadec-12(Z)-enoic acid (Supplementary Table S1), which are involved in development and responses to stress43.
Several data from the metabolomic analyses of plants exposed to NO suggested that one aspect of the global stress responses activated by NO is the promotion of cell death. The transient but strong increase in pheophorbide a content detected in NO-exposed plants (Supplementary Fig. S1b) is consistent with the promotion of cell death as previously reported35. Also consistent with the promotion of cell death, many cells of roots from NO-treated plants were permeable to propidium iodide showing strongly stained nuclei (Fig. 5a). Metabolomic data also pointed to the massive protein and nucleic acid degradation in plants exposed to NO, and these catabolic processes are usually activated under cell death conditions44,45. Although we found a pattern of progressive DNA degradation upon NO treatment, no DNA ladders were detected (Supplementary Fig. S1a), thus suggesting cell death is not apoptotic but more likely necrotic. Accordingly, we have detected cell death in plants by Evans blue staining at 6 h and 24 h after exposure to NO (Fig. 6c). It has been reported that cell death can be delayed by polyamines46, so the increase in polyamines detected in NO-treated plants (Fig. 3) may represent a strategy to attenuate NO-triggered cell death. Moreover, not only protein breakdown but also tyrosine nitration-based protein modification has been characterized as a marker of cell death signaling pathways47. Accordingly, we found a strong increase in tyrosine protein nitration after exposure of plants to NO (Fig. 2b). In animals models, the nitration of proteins often favors subsequent proteolytic degradation48,49 and, in plants, we have recently reported that nitration of ABA receptors triggers their inactivation, polyubiquitylation and subsequent proteasomal degradation50. Although more work is required to substantiate the connection between NO, protein nitration and protein breakdown, the work presented here supports it. On the other hand, the functional connection between NO and cell death is complex in animal systems being reported both pro- and anti-apoptotic activities51,52 likely depending on the concentration. Similarly in plants, NO has both promoting and suppressing effects on cell death, depending on cell type and redox status, as well as on the NO dose53. NO has been characterized as a key regulator of programmed cell death during self-incompatibility events essential to prevent self-fertilization54. Maize cells with suppressed hemoglobin, enhanced nitric oxide and reduced auxin levels are committed to die during embryogenesis55. Another support of NO triggering cell death might come from the increased metabolism of nucleic acids detected in NO-exposed plants (Supplementary Table S1), which runs in parallel with genomic DNA degradation (Fig. S1a) in cells that are committed to die. However, NO-exposed plants despite of displaying multiple metabolic and cellular symptoms of cell death by 6 h, were fully viable and actually undistinguishable from untreated plants by 24 h and, by 7 days after exposure to NO, 100% of the treated plants survived and grew normally (Fig. 6c). These data suggest NO only triggers cell death under natural stress conditions in a sharply localized way in places where the intracellular concentration is above a threshold level.
A small but statistically significant increase (between 1.2 and 1.5-fold) in a large collection of dipeptides was also detected by 6 h after NO treatment (Supplementary Table S1). The accumulation of dipeptides may be the result of the N-terminal dipeptide release from target proteins, a process that in mammals is associated to cell differentiation, the protection from cell death, and to have a role in the degradation of oligopeptides from proteins such as collagen56. However, the levels of all identified dipeptides were not significantly high by 24 h after NO treatment (Supplementary Table S1), thus indicating all dipeptides should be degraded without an increase in free amino acid content at that time point. Another reason for the accumulation of dipeptides could be related to a plant response directed to ameliorate the combined effects of NO and O2 in the cysteine branch of the N-terminal targeted proteolysis of certain protein substrates through the N-end rule pathway, a process that we have recently characterized as a sensor mechanism of NO in Arabidopsis57. It is well known that certain dipeptides inhibited the proteolysis of targeted proteins through the N-end rule pathway58, thus allowing us to posit that an increase in endogenous levels of dipeptides might be used for the plant to protect some key proteins from specific N-terminal directed proteolysis.
All together, data presented here suggest that NO likely triggers an array of responses to alleviate toxicity and cellular damage that includes massive but transient metabolic reprogramming involving metabolites of both primary and secondary metabolism.
Plant materials, growth conditions and NO treatment
Wild type Col-0 and the NO-deficient nia1nia2noa1-2 mutant seeds of Arabidopsis thaliana were sown in moistened soil and grown under photoperiod cycles of 16 h day and 8 h night (long days, at 22 °C and 20 °C, respectively), under 150 μE m-2 s-1 cool-white fluorescent lamps and 60% relative humidity. Alternatively, surface sterilized seeds were sown after 4 d of stratification at 4 °C under darkness and grown in agar-supplemented Murashige and Skoog (MS) medium (Duchefa, Haarlem, The Netherlands) supplemented with 1% (w/v) sucrose.
The pulse of NO was performed by incubating plants for 5 min in a tightly sealed transparent box after injection of 300 ppm of pure NO gas (Linde AG, Germany).
Cuticle permeability tests
The toluidine blue test was carried out by placing 10 μl droplets of a 0.025% (w/v) toluidine blue solution in potato dextrose broth (PDB) on the upper side surface of leaves. After 2 h leaves were washed gently with distilled water to remove excess of the toluidine blue solution, and then leaves were excised and photographed. For Calcofluor white staining, leaves were bleached in absolute ethanol overnight, equilibrated in 0.2 M phosphate buffer (pH 9) for 1 h, and incubated for 1 min in 0.5% (w/v) Calcofluor white in 0.2 M phosphate buffer (pH 9). Leaves were rinsed in phosphate buffer to remove excess of Calcofluor white and viewed under UV light.
Staining for cell permeability, cell death, NO, superoxide and starch
To assess cell permeability, roots of 10 d-old seedlings were stained with propidium iodide by dipping into a 10 μg/ml solution for 10 min. Stained roots were visualized under confocal microscopy with excitation at 488 nm and emission at 598–650 nm range. Cell death was assayed by staining seedlings with 2% (w/v) Evans blue solution for 5 min and subsequent extensive washing. The endogenous levels of NO were assayed by staining with DAF-FM DA, and the NO-associated fluorescence was detected with a fluorescence Leica DM 5000B microscope as described32. Superoxide staining was performed by dipping seedlings in 0.2% (w/v) solution of nitroblue tetrazolium (NBT) in 50 mM sodium phosphate buffer (pH 7.5) overnight at room temperature and protected from light followed by bleaching with hot ethanol. Staining of starch granules was performed by bleaching 7d-old seedlings with 96% ethanol for 5 h and then by incubating bleached seedlings in Lugol’s staining solution (5.7 mM iodine, 43.4 mM KI, 0.2N HCl) for 30 min and further extensive overnight washing with water.
Protein extraction and Western blot analyses
Seedlings frozen in liquid nitrogen were homogenized with a polytron in 0.1 M Tris-HCl buffer pH 8 containing 150 mM NaCl, 0.1% (v/v) NP-40 (Roche) and 1% (v/v) protease inhibitor cocktail (Sigma). Supernatants of 15 min centrifugation at 16000 × g and 4 °C, were quantified with Bradford reagent and separated under SDS-PAGE. Gels were either stained for total protein with Imperial reagent (Thermo Scientific) or semi-dry transferred to nitrocellulose membranes. After blocking membranes with TTBS buffer supplemented with either 5% (w/v) non-fat dried milk, 3% (w/v) Top-Block (Fluka) or 3% (w/v) bovine serum albumin, Western blots were performed by incubating membranes overnight at 4 °C in the following primary antibodies at the indicated dilution factor: monoclonal anti-3nitroY (Cayman Chemicals, 1:1000) and monoclonal anti-TMT (Thermo Scientific 1:1000). Polyclonal anti DE-ETIOLATED 3 (DET3) (kindly donated by David Alabadí, IBMCP Valencia Spain, at 1:10000) was used as loading control59. Secondary anti-rabbit or anti-mouse antibodies coupled to horseradish peroxidase (GE, 1:5000) and Supersignal WestPico Chemiluniscence reagents (Thermo Scientific) were used to visualize bands.
DNA isolation and Southern blot
Genomic DNA was isolated with hexadecyltrimethylammonium bromide (CTAB)-containing buffer, and 15 μg were separated through 2% agarose gel, blotted onto positively charged nylon membranes, and Southern blot performed with a digoxigenin-labelled probe (DIG High Prime DNA Labeling and Detection Starter Kit II from Roche).
The sample preparation process was carried out using the automated MicroLab STAR® system from Hamilton Company. Recovery standards were added prior to the first step in the extraction process for quality Control (QC) purposes. Sample preparation was conducted by series of organic and aqueous extractions to remove the protein fraction while allowing maximum recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by Liquid Chromatography (LC) and one for analysis by Gas Chromatography (GC). Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen, dried under vacuum and prepared for either LC/MS or GC/MS. Details on the methodology used for the metabolomic analyses is included in the Metabolomic Procedure sheet of Supplementary Table S1.
For metabolomic analyses, following log transformation and imputation with minimum observed values for each compound, Welch’s two-sample t-test and ANOVA contrast were used to identify biochemicals that differed significantly between experimental groups. A Two-way ANOVA was also used to identify biochemicals exhibiting a significant time and treatment main effect and the interaction effect between these two variables. An estimate of the false discovery rate (q-value) was calculated to take into account the multiple comparisons. Statistical analyses are performed with the program “R” http://cran.r-project.org/.
How to cite this article: León, J. et al. Nitric oxide triggers a transient metabolic reprogramming in Arabidopsis. Sci. Rep. 6, 37945; doi: 10.1038/srep37945 (2016).
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This work was supported by grants BIO2011–27526 and BIO2014-56067-P from the Spanish Ministry of Economy and Competitiveness and FEDER funds. We thank support and comments from Danny Alexander (Metabolon Inc., USA) on metabolomic analyses.