Nonsense-mediated mRNA decay (NMD) is a cellular process that eliminates messenger RNA (mRNA) substrates with premature translation termination codons (PTCs). In addition, NMD regulates the expression of a number of physiological mRNAs, for example transcripts containing long 3′ UTRs. Current models implicate the interaction between cytoplasmic poly(A)-binding protein (PABPC1) and translation termination in NMD. Accordingly, PABPC1 present within close proximity of a termination codon antagonizes NMD. Here, we use reporter mRNAs with different NMD-inducing 3′ UTRs to establish a general NMD-inhibiting property of PABPC1. NMD-inhibition is not limited to PABPC1, but can also be achieved by peptides consisting of the PABP-interacting motif 2 (PAM2) of different proteins when recruited to an NMD-inhibiting position of NMD reporter transcripts. The short PAM2 peptides efficiently suppress NMD activated by a long 3′ UTR, an exon-junction complex (EJC) and individual EJC components, and stabilize a PTC-containing β-globin mRNA. In conclusion, our results establish short PABPC1-recruiting peptides as potent but position-dependent inhibitors of mammalian NMD.
Gene expression is an essential and highly regulated process in eukaryotic cells. Several quality control mechanisms are present in the cell to ensure that the correct information is conveyed from DNA level to protein level. One important surveillance mechanism is nonsense-mediated mRNA decay (NMD), which detects and removes erroneous mRNA transcripts such as transcripts harboring premature translation termination codons (PTCs)1,2,3. In mammalian cells PTCs are recognized and NMD is activated if the PTC is located >50 nucleotides upstream of an exon-junction complex (EJC)4,5, a protein complex deposited on the mRNA during splicing6. The central NMD factor UPF1 plays a pivotal role in NMD initiation and serves as an interaction hub for several other NMD factors7,8,9. After the recognition of a PTC, a cascade of events initiates the degradation of the mRNA by the recruitment of the heterodimer SMG5/SMG7 and SMG610.
NMD also plays a role in regulating expression of various physiological mRNAs. However, only some of these endogenous NMD targets contain PTCs, which can potentially result from alternative splicing or upstream open reading frames11,12. Besides PTC-containing transcripts, several long 3′ UTR containing mRNAs are known to be endogenous NMD targets12,13,14. It has been shown that the 3′ UTR of some endogenous NMD targets are sufficient to induce degradation of the transcript15. The mere length of a 3′ UTR has been excluded as the determining factor for degradation of a long 3′ UTR-containing transcript as a study has shown that some long 3′ UTRs are degraded by NMD whereas others are not16. The same study suggests instead that the AU-content of a 3′ UTR could potentially mediate the binding of a cis factor in close proximity of the termination codon16. Recruiting a cis factor to the termination codon could possibly suppress activation of NMD. However, it is currently still unknown how a 3′ UTR is marking the transcript for NMD activation or NMD evasion.
It has previously been shown that the cytoplasmic poly(A)-binding protein (PABPC1) can suppress NMD-mediated degradation of a reporter when recruited in close proximity of a termination codon13,17,18,19,20. Earlier models suggested that the central NMD factor UPF1 competes with PABPC1 for interaction with eukaryotic release factor 3a (eRF3a). If PABPC1 is too far downstream of a terminating ribosome, UPF1 is believed to interact with eRF3a instead. In turn, this is believed to recruit additional NMD factors and will eventually lead to the degradation of the transcript14. Two independent studies have shown, however, that the C-terminal domain of PABPC1, which mediates the interaction with eRF3a, is dispensable to suppress NMD of a reporter targeted by NMD17,18. These findings contradict the competition model between PABPC1 and UPF1 for eRF3a binding as a determining factor for NMD activation. Instead, it has been shown that the interaction between PABPC1 and eukaryotic initiation factor 4 G (eIF4G) is necessary to suppress NMD17,18. This suggests that an efficient translation termination event could be the responsible determinant for whether NMD is activated or not. The involvement of eIF4G in these findings also suggests a potential role of the closed loop mRNA conformation and ribosome recycling in NMD suppression. Taken together, these findings advocate that the determining factor for NMD suppression might be the recruitment of PABPC1 into close proximity of a terminating ribosome, followed by efficient translation termination and ribosome recycling.
Eukaryotic release factor 3a can interact with PABPC1 via a so-called PABP-interacting motif 2 (PAM2 motif). A region in the PABPC1 C-terminus termed Mademoiselle (MLLE) domain has been shown to specifically recognize PAM2 motifs21. Essential amino acids (Glutamate, Phenylalanine, Proline) in the PAM2 motif are conserved among several PAM2 motifs from different polypeptides22. The PAM2 motif was first discovered in PABP-interacting protein 1 (PAIP1), PAIP2, and eRF3a23. More proteins harboring PAM2 motifs have been identified since the discovery of the PAM2 motif23,24. Different PAM2-containing proteins play roles in different cellular processes. For example, the interaction between PABPC1 and eRF3a has been shown to play a role in polypeptide release and ribosome recycling22,25,26. On the other hand, the PAM2-containing proteins PAN3 and TOB1 play a role in deadenylation. PABPC1 recruits the PAN2-PAN3 deadenylase directly by interacting with PAN327. The other deadenylase, CCR4-NOT, is not directly recruited to the mRNA via PABPC1 but through TOB1 interacting with PABPC128. Furthermore, the PABPC1-interacting protein PAIP2 is a known repressor of translation29. It is interesting to note that PABPC1 can interact with a multitude of PAM2-containing proteins with potentially contradictory functions. It is currently unknown how the interaction between PABPC1 and the many PAM2-containing proteins is regulated.
Many genetic disorders are caused by nonsense mutations, for which NMD acts as a modifier and eliminates truncated proteins with potential deleterious effects30,31,32. However, not all truncated proteins have a negative effect. In some instances, increased amounts of a truncated protein can lead to a less severe form of a disease33. Suppressing NMD could be favorable in medical conditions where the presence of a truncated protein would be advantageous over the complete absence of the protein. Elevated levels of full length proteins could be achieved by a combined treatment with drugs that induce translational read-through32.
Earlier studies reported that recruiting PABPC1 into close proximity of a termination codon suppresses NMD. Here, we investigated different methods of NMD suppression via PABPC1 recruitment. We find that the recruitment of PABPC1 by PAM2 peptides inhibits NMD of reporter transcripts. These results open up new ways of NMD suppression by small biologically active peptides.
Translation and efficient translation termination play a pivotal role in gene expression and the lifespan of an mRNA. PABPC1 is an essential factor during this process, promoting mRNA circularization via its interaction with eIF4G, suppressing NMD at normal termination codons, and facilitating ribosome recycling20,34,35,36. A revised model of how PABPC1 can possibly act to suppress NMD when in close proximity of a termination codon was previously proposed17,18,19. Here, we further investigate mechanisms of NMD suppression by PABPC1.
PABPC1 and eRF3a suppress NMD of additional long 3′ UTR containing reporter mRNAs
We have previously reported that both PABPC1 and eRF3a are able to suppress NMD of a reporter carrying the long 3′ UTR of SMG517. We next aimed to generalize this observation, since our previous findings were based on a single reporter mRNA with the SMG5 3′ UTR. To this end, we constructed two tethering reporters containing the 3′ UTRs of PDRG1 and SURF6 downstream of 4MS2 binding sites (Fig. 1a). Both, PDRG1 and SURF6 were identified by a transcriptome-wide screen in our laboratory as endogenous NMD substrates (unpublished data Gehring lab). As expected, both PABPC1 and eRF3a were able to antagonize degradation via NMD of both reporter constructs and increased the mRNA levels about 3-fold compared to a negative GST-control (Fig. 1b and c). Hence, NMD suppression by both PABPC1 and eRF3a is a general phenomenon that can act on different substrate mRNAs targeted by NMD due to their 3′ UTR. To show that PDRG1 and SURF6 reporters are targeted by NMD, we knocked down the NMD factor UPF2 in cells transfected with each reporter. This led to an increase in PDRG1 and SURF6 reporter mRNA levels of about 3.5- and 4-fold compared to a luciferase control knockdown, respectively (Fig. 1d and e).
Suppressing NMD by indirectly recruiting PABPC1
It has previously been shown that eIF4G is able to suppress NMD, when tethered in close proximity of a termination codon17,18, even when lacking the PABPC1 interaction site. Here, we made use of an eIF4G deletion mutant (eIF4G 84–295), which only contains the interaction site for PABPC1. The short form of eIF4G was still able to suppress NMD of a reporter containing the SMG5 3′ UTR, which could be seen by a 5-fold increase in reporter mRNA (Fig. 2b). This result suggests that eIF4G is able to suppress NMD with no other factors involved, but simply by recruiting PABPC1. We previously showed that eIF4G is able to suppress NMD without the need for PABPC117. In contrast, we show here that NMD can be suppressed by simply recruiting PABPC1 to an NMD-targeted reporter via a short version of eIF4G that does not interact with any other protein but PABPC1. The fact that the deletion mutant of eIF4G, containing only the PABPC1 interaction region, is able to suppress NMD, suggests that recruiting PABPC1 via a short peptide is sufficient for NMD inhibition.
PABPC1 contains a well-conserved 70 amino acid domain termed MLLE in its C-terminal region23. It has been shown that this MLLE domain specifically recognizes the small peptide sequence called PAM2 motif21. Initially, three different proteins were identified carrying a PAM2 motif: PAIP1 (PABP-interacting protein 1), PAIP2, and eRF323. To assess the NMD suppression potential of recruiting PABPC1 via short peptides, we made use of the isolated PAM2 motif of PAIP2 to recruit PABPC1 to the reporter mRNA (Fig. 2c). Interestingly, recruiting PABPC1 via the PAIP2 PAM2 motif suppresses NMD and results in increased reporter mRNA levels of about 4-fold (Fig. 2c, lane 2). An MS2V5-tagged version of SYNZIP 1, a short artificial peptide with no inherent function, was used as a negative control (Fig. 2c, lane 1)37. To assess whether PABPC1 crowding near a termination codon has an effect on NMD suppression, we cloned two and four PAIP2 PAM2 motifs separated by short spacers in succession and tethered these to the reporter mRNA. Tethering MS2V5-tagged PAIP2 PAM2 2x and PAIP2 PAM2 4x to the reporter did not lead to an additional increase in mRNA reporter levels compared to MS2V5-PAIP2 PAM2 (Fig. 2c, compare lane 2 to lanes 3 and 4). This shows that recruiting more PABPC1 via multiple PAM2 motifs in succession to an NMD-inhibiting position on a reporter mRNA does not result in an increased NMD suppression compared to a single PAIP2 PAM2 motif. Instead we suggest that tethering a PAM2 motif just downstream of a termination codon leads to the folding back of the poly(A) tail by PAM2 interacting with poly(A)-bound PABPC1. This would also explain the lack of increased NMD suppression by employing multiple PAM2 motifs in succession, as one PAM2 motif is enough to interact with and fold back poly(A)-bound PABPC1.
Tethering PAM2 peptides to an NMD-targeted reporter suppresses degradation
So far we have established that the PAM2 motif of PAIP2 is able to suppress NMD of a long 3′ UTR reporter (Fig. 2). Other PAM2-containing proteins have been identified based on sequence homology22. Interestingly, eRF3 is different compared to other PAM2-containing proteins since it carries two overlapping PAM2 motifs21. Since PABPC1 strongly interacts with proteins harboring a PAM2 motif, we tethered the isolated PAM2 motifs of several proteins to recruit PABPC1 to an NMD-targeted reporter (Fig. 3a). Tethering the MS2V5-tagged overlapping N- and C- terminal eRF3a PAM2 motif to the previously used FLAG-TPI-4MS2-SMG5 reporter increased the levels of the reporter mRNA about 5-fold (Fig. 3b, lane 2)17. As a negative control, we used a point mutation of the PAM2 motif of eRF3a (eRF3a F76A) known to reduce the binding affinity between eRF3a and PABPC122,38,39. Tethering MS2V5-eRF3a F76A PAM2 to the reporter mRNA had a much smaller effect compared to eRF3a containing both PAM2 motifs (Fig. 3b, lane 3). Tethering the N- and C-terminal PAM2 motifs of eRF3a separately to the NMD-targeted reporter increases the reporter mRNA levels also about 5-fold (Fig. 3b, lanes 4 and 5). The PAM2 motifs of NF-X1, Ataxin2, and PAIP2 also increased the reporter mRNA levels by 5- to 8-fold upon tethering (Fig. 3b, lanes 8, 9, and 10). Surprisingly, tethering MS2V5-tagged TOB1 and PAN3 PAM2 motifs to the reporter only led to a small increase of the reporter mRNA (Fig. 3b, lanes 6 and 7).
The poly(A) tail and PABPC1 are considered to determine translation efficiency in eukaryotes40. Given the strong increase in mRNA levels upon tethering PAM2 motifs to the reporter we wanted to exclude that NMD is indirectly suppressed due to the inhibition of translation. To this end we employed the N-terminal FLAG-tag of the TPI reporter to quantify protein expression compared to FLAGemGFP, which was transfected as control for protein expression (Fig. 3c, lower panel). The protein levels upon tethering were increased approximately 2-fold for the eRF3a, as well as the NF-X1, Ataxin2, and PAIP2 PAM2 motifs (Fig. 3c). In contrast, tethering of the less active eRF3a F76A PAM2 motif as well as PAM2 of TOB1 and PAN3 led to a slightly less increase in protein expression from the reporter mRNA (Fig. 3c). The implication of this smaller increase in protein abundance remains unclear.
It is surprising that some PAM2 peptides are very efficient inhibitors of NMD, while others show only very little activity. We hypothesized that differences in the interaction of the PAM2 peptide with PABPC1 are causing the observed differences. To this end, we expressed FLAGemGFP-tagged PAM2 motifs and subjected them to a FLAG pulldown. We used an antibody against endogenous PABPC1 to check for interactions between PAM2 motifs and PABPC1. The strongest interaction was observed for the overlapping eRF3a PAM2 motifs (Fig. 3d, lane 2, right panel), although the eRF3a PAM2 motif consistently exhibited very low expression levels. As expected, eRF3a F76A PAM2 pulled out significantly less endogenous PABPC1 (Fig. 3d, lane 3, right panel). The residual interaction between eRF3a F76A and endogenous PABPC1 can be attributed to the second Phenylalanine still present in this mutant. Furthermore, strong interactions between eRF3a -N PAM2, eRF3a -C PAM2, Ataxin2 PAM2, PAIP2 PAM2, and endogenous PABPC1 were observed (Fig. 3d, lanes 4, 5, 6, and 7, right panel). NF-X1 PAM2 and TOB1 PAM2 pulled out less endogenous PABPC1, whereas PAN3 PAM2 did not interact with endogenous PABPC1 at all (Fig. 3d, lanes 8, 10, and 9, right panel). These results explain the inefficient NMD inhibition by the PAM2 motifs of PAN3, and the reduced NMD suppression by TOB1 and the negative control eRF3a F76A PAM2. It is surprising that some PAM2 motifs are unable to interact with PABPC1 even though the essential amino acids for the interaction with PABPC1 are present. Interestingly, PAIP2 PAM2 was able to stabilize the reporter mRNA more strongly than the other PAM2 motifs. The reason behind these different interaction and stabilization patterns is currently unknown.
PAM2-mediated NMD suppression is position dependent
Next, we made use of several different control reporter constructs to test the specificity of tethering different PAM2 motifs and to exclude non-specific trans effects. To this end we used a reporter lacking any MS2-binding sites and a reporter carrying MS2-binding sites downstream of the SMG5 3′ UTR (Fig. 4a). We used the eRF3a, Ataxin2, and PAIP2 PAM2 motifs for this assay since these have shown the strongest NMD-antagonizing effect of the PAM2 motifs studied here. First, we tethered the three PAM2 motifs separately to the TPI-4MS2-SMG as a positive control, resulting in an increase in mRNA reporter abundance (Fig. 4b). Tethering the PAM2 motifs to the reporter without any MS2-binding sites or with the MS2 sites downstream of the SMG5 3′ UTR did not lead to an increase in mRNA abundance (Fig. 4c and d) indicating the specificity of the tethering assay. Western blotting showed the expression of all constructs to be similar and excludes a lack of expression as a possibility for failed reporter mRNA stabilization (Fig. 4e).
PAM2 peptides efficiently antagonize EJC-mediated NMD
So far we have shown that PAM2 motifs are able to efficiently suppress NMD activated by the SMG5 3′ UTR by recruiting PABPC1 into close proximity of the termination codon. However, many NMD substrates are not targeted due to a 3′ UTR, but are activated by the splicing-dependent EJC, which is deposited at exon-exon junctions. To address whether PAM2 mediated NMD suppression can also suppress NMD activated by an EJC, we next made use of a TPI reporter carrying an intron (MINX) downstream of the 4MS2-binding sites and upstream of the SMG5 3′ UTR (Fig. 5a). During RNA processing the intron will be spliced out of the transcript and an EJC is deposited downstream of the 4MS2 sites. We previously reported that tethering PABPC1 to this reporter does not lead to an increase in mRNA abundance17. Here, we observed a 5 to 6-fold increase in reporter mRNA abundance upon tethering eRF3a, Ataxin2, and PAIP2 PAM2 motifs upstream of the MINX intron (Fig. 5b). Tethering MS2V5-PABPC1 to this reporter led to significantly less stabilized reporter mRNA levels compared to the PAM2 motifs (Fig. 5b, compare lane 5 to lanes 2–4). These results establish that recruiting PABPC1 through a small PAM2 motif leads to significant NMD inhibition.
The reporter that we have used before (Fig. 5a) contains both an intron and a long 3′ UTR. To directly test the ability of PAM2 peptides to suppress NMD by an EJC, we chose to activate NMD by tethering of two EJC-factors: RNPS1 and Barentsz (BTZ or MLN51). RNPS1 was first identified as a general splicing activator and is known to initiate NMD when tethered to a reporter41,42. Similarly, BTZ, part of the EJC core complex, has also previously been shown to initiate NMD of a reporter upon tethering to the construct43. For this experiment, we constructed a double tethering reporter harboring 4MS2 binding sites upstream of 4PP7 binding sites (Fig. 5a). This double tethering reporter allows us to tether two different fusion proteins in a specific spatial orientation. When tethering the PAM2 motif of PAIP2 upstream of RNPS1 we observed an approximately 6-fold increase of reporter mRNA levels compared to tethering RNPS1 downstream of GST (Fig. 5c). Likewise, NMD initiated by tethering BTZ was suppressed by tethering the PAM2 motif of PAIP2 upstream of BTZ (Fig. 5d). Tethering PAIP2 PAM2 in this spatial orientation leads to an 8-fold increase in reporter mRNA levels compared to BTZ tethered downstream of GST (Fig. 5d). These results indicate that the PAM2 motif of PAIP2 is not only able to suppress 3′ UTR-mediated but also EJC-mediated NMD. In combination, these results are a strong indication for an NMD suppression ability of PAM2 motifs when tethered upstream of an EJC.
Recruiting PABPC1 suppresses NMD of a PTC-containing mRNA
As we have so far shown that PABPC1 recruited via a tethered PAM2 motif can strongly suppress NMD either activated by a long 3′ UTR or a downstream EJC, we next wanted to examine the ability of PAM2 peptides to suppress NMD of a PTC-containing mRNA. Human β-globin carrying a PTC at position 39 has been identified in patients suffering from β-thalassemia and is a well-known NMD target4,44,45. We used a reporter with MS2 binding sites inserted downstream of the PTC at position 39. The remainder of the β-globin DNA was cloned after the MS2 binding sites (Fig. 6a). This reporter construct allows us to tether an NMD antagonizing factor in a close to natural setting. This reporter carries an intron downstream of the PTC, which results in an EJC deposited downstream of the terminating ribosome, thereby initiating NMD. Tethering MS2V5-PABPC1 to this reporter leads to an increase in mRNA reporter abundance by about three fold (Fig. 6b, lane 2). The same magnitude of stabilization is observed when tethering the PAM2 motifs of eRF3a and PAIP2 to the reporter (Fig. 6b, lanes 3 and 4). Taken together these results establish that PAM2 peptides have the capability to suppress NMD of an endogenous NMD target when bound at a position downstream of the termination codon and upstream of the next NMD-activating EJC.
Mammalian NMD degrades mRNAs that carry an EJC downstream of the termination codon or that contain a long 3′ UTR. It has been previously demonstrated that the physical distance between the termination codon and the poly(A)-tail is important to discriminate between a proper and an aberrant termination event13. Different mechanisms of NMD evasion have been previously described for NMD substrates. Firstly, a 200nt A/U-rich region immediately downstream of a termination codon has been shown to be responsible for NMD suppression16. This region could potentially be bound by an as of yet unknown protein or proteins, which are either able to block NMD by disrupting the interaction with eRF3a and UPF1, or by interacting with PABPC1 directly. Recently, PTBP1 was identified as such a protein that binds to some mRNAs with a long 3′ UTR just downstream of the termination codon, thereby blocking NMD46. PTBP1 binds preferentially to CU-rich regions and a single PTBP1 protein has multiple RNA binding sites. This way it is conceivable that PTBP1 could compact the 3′ UTR by binding stretches of the 3′ UTR further apart. By doing so PABPC1 would be brought into closer proximity of the terminating ribosome, which could lead to NMD suppression. It has also been previously reported that tethering PABPC1 or inserting a stretch of adenosines immediately downstream of a PTC suppresses NMD by reducing the binding of UPF1 to the mRNA47. Hence, PABPC1 may be involved in the active dissociation of UPF1 from stable, non-target mRNAs.
The strongest NMD antagonizing effect we observed here resulted from tethering PAM2 peptides to a reporter construct targeted by NMD (Fig. 3). Specifically, the 15 amino acid PAM2 motif of PAIP2 showcased the strongest suppression of NMD induced by the SMG5 3′ UTR. The results obtained by tethering PAM2 motifs to the NMD reporter were surprising. For instance, recruiting PABPC1 via the PAIP2 PAM2 peptide to the SMG5 3′ UTR reporter resulted in a 3-fold higher mRNA abundance compared to what we observed in our previous work when tethering PABPC1 directly17. We have currently no explanation as to why this difference in NMD suppression strength is occurring. Besides PABPC1, the PAM2 motif is known to only interact with EDD, the human homolog of the Drosophila tumor suppressor gene hyperplastic discs (hyd)48. EDD has been shown to share a 56% similarity of the PABP C-terminal domain and is able to interact with PAIP149. However, to our knowledge EDD is not implicated in the NMD mechanism and therefore we postulate that the NMD inhibiting effects of PAM2 motifs is mediated by their binding to PABPC1. A possible theory as to why we observe a much stronger stabilization by tethered PAM2 motifs could be that the MLLE domain of PABPC1 is blocked when PABPC1 is recruited to the reporter via a tethered PAM2 motif. In principle, when PABPC1 is tethered directly it could theoretically still interact with other PAM2 carrying proteins, which might regulate its NMD suppression ability. Obstructing the MLLE domain of PABPC1 when it is recruited via a PAM2 motif would remove the possibility of other PAM2-carrying proteins interacting with PABPC1 and possibly reducing its NMD-inhibiting effect. This hypothesis would also be in line with the previous observation that PABPC1 does not require its MLLE domain for NMD suppression17,18. Our result that tethering of multiple PAM2 motifs does not enhance NMD suppression suggests an alternative explanation for the remarkable efficiency of PAM2 peptides. Because a single PAM2 suffices to suppress NMD, a tethered PAM2 peptide may fold back the poly(A) tail by interacting with poly(A)-bound PABPC1. A similar fold-back may be exerted by the PABPC1-interacting domain of eIF4G, which is also a potent inhibitor of NMD in our hands. Folding back the poly(A) tail has been shown to strongly suppress NMD13, but its mechanism has not been studied in detail. Nonetheless, it is likely that NMD inhibition by the poly(A)-PABPC1 entity involves the interaction of PABPC1 with eIF4G, which has been suggested to promote ribosome recycling.
It was also surprising to see that not all PAM2 motifs have the same capability to antagonize NMD. For example, the TOB1 and PAN3 PAM2 motifs do not stabilize the SMG5 3′ UTR reporter construct (Fig. 3b). The most likely explanation for this is the lack of interaction of these two PAM2 motifs with endogenous PABPC1 (Fig. 3d). This is puzzling as the 15 amino acid PAM2 peptides of these two proteins contain all three of the crucial amino acids of a PAM2 motif required for the interaction with PABPC1. More work will be required to understand the determinants of the interaction between PAM2 peptides and PABPC1 under cellular conditions.
Many inherited diseases are caused by frameshift or nonsense mutations leading to transcripts carrying a PTC. These transcripts activate NMD due to one or more EJCs downstream of the termination codon. Here we show that inhibiting NMD by tethering of a PAM2 motif is not limited to 3′ UTR activated NMD. We have shown here that PAM2 peptides are able to suppress NMD activated by either an EJC deposited during splicing or by single EJC components (Fig. 5). Furthermore, tethering PAM2 peptides downstream of PTC39 in the β-globin mRNA inhibited NMD of this well-established NMD reporter. The fact that a small PAM2 motif can suppress EJC-mediated NMD is surprising as we previously reported that full length PABPC1 is unable to suppress EJC NMD when tethered upstream of an EJC17. It will be an important task for future studies to elucidate the differences between EJC- and 3′ UTR-activated NMD and their suppression by PABPC1 and PAM2 peptides.
We have established tethering of PAM2 motifs as a new means for specifically antagonizing the degradation of NMD substrates. Recruiting PABPC1 in close proximity of a termination codon via a small PAM2 peptide suffices to inhibit both 3′ UTR- and EJC-mediated NMD. Due to their small size, PAM2 peptides are ideal for the large-scale synthesis of biologically active components and their activity may be further enhanced by synthetic modifications and/or mutagenesis. So far, we have only demonstrated the activity of PAM2 motifs by tethered function analysis. However, PAM2 peptides also may be combined with sequence-specific RNA-binding molecules, such as Pumilio homology domains50,51, CRISPR-Cas952,53 or antisense oligonucleotides54 to suppress NMD independent of tethering. Applied in combination with read-through drugs, PAM2 peptides may also enable to restore the expression of nonsense-mutated genes. Future studies will help to exploit the remarkable NMD-inhibiting potential of PAM2 peptides in therapeutic applications.
Plasmid constructs β-globin, pCI-FLAGemGFP, pCI-MS2V5, pCI-mVenus, pCI-TPI and expression vector for PABPC1 and eIF4G were described previously55,56,57,58,59. The modification of pCI-TPI with four copies of binding sites for the heterologous probe used in northern blot analysis, as well as the insertion of the SMG5 3′ UTR and 4MS2 binding sites in the 3′ UTR of TPI were described elsewhere15. Using the same cloning strategy three more reporters were created: the 3′ UTR of PDRG1 and SURF6 were introduced into the TPI vector, and 4PP7- and 4MS2-binding sites enclosing the SMG5 3′ UTR were cloned into the TPI vector. The β-globin-PTC39–4MS2-β-globin reporter was constructed by inserting 4MS2-binding sites at position 112 nucleotides into the second exon of β-globin. The remainder of β-globin was inserted downstream of these 4MS2-binding sites. The MINX-containing reporters, as well as the SMG5 3′ UTR reporter containing alternate termination codon sites were described previously17. Deletion and point mutants of eRF3a and eIF4G were generated by site-directed mutagenesis. The PAM2 containing mutants were generated by PCR and cloned in the designated expression vector. All plasmid constructs were verified by sequencing.
PAM2 plasmid construction
PAM2 motifs were cloned by PCR using two overlapping primers. The finished PCR products were digested with XhoI and NotI, followed by ligation into either pCI-MS2V5 XhoI/NotI or pCI-FLAGemGFP XhoI/NotI.
HeLa cells were grown in 6-well plates or 6 cm dishes at concentrations of 240,000 cells/ml and 650,000 cells/ml, respectively. Transfections were done by calcium phosphate precipitation. For tethering and overexpression experiments 0.3 μg of an mVenus expression plasmid, 0.5 μg control plasmid (β-globin), 2.0 μg plasmid encoding for reporter mRNA, and 0.8 μg of MS2V5-, PP7∆FGV5-, and λNV5-expression plasmids were used in the transfection mix. For co-immunoprecipitation experiments 1.5 μg FLAGemGFP-expression plasmids and 1.0 μg Salmon DNA were added to a transfection mix. For translation efficiency experiments we included 1.5 μg FLAGemGFP as control and 2.0 μg FLAG-TPI-SMG5-4H as reporter in the transfection mix.
Delivery of siRNAs into cells was performed by reverse transfecting 60 pmol siRNA per 2 × 105 cells using 2.5 μl Lipofectamine RNAiMAX (Thermo Fisher). The following siRNA target sequences were used: luciferase (5′-CGTACGCGGAATACTTCGA-3′) and UPF2 (5′-CGTTGTGGATGGAGTGTTA-3′).
RNA extraction and northern blotting
Total RNA was extracted with peqGOLD TriFast lysis reagent (Peqlab) and analyzed by northern blotting as described56. Signals were quantified using a Typhoon FLA 7000 (GE Healthcare).
SDS-polyacrylamide gel electrophoresis and immunoblot analysis was performed using protein samples derived from peqGOLD Trifast extractions. The antibody against FLAG (F7425; 1:3000 dilution) was from Sigma, the antibody against V5 (18870; 1:3000 dilution) was from QED Bioscience, the antibody against GFP (ab290; 1:3000 dilution) was from Abcam, the antibody against PABPC1 (#4992; 1:3000 dilution) from Cell Signaling Technology, the antibody against tubulin (T6074; 1:3000 dilution) was from Sigma, and the antibody against UPF2 was kindly provided by Jens Lykke-Andersen (1:1000 dilution). Secondary horseradish peroxidase-coupled antibodies against rabbit (111-035-006; 1:3000 dilution) or mouse (115-035-003; 1:3000 dilution) were from Jackson ImmunoResearch. Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate (PerkinElmer) in combination with the myECL Imager (ThermoFisher) was used for visualization.
How to cite this article: Fatscher, T. and Gehring, N. H. Harnessing short poly(A)-binding protein-interacting peptides for the suppression of nonsense-mediated mRNA decay. Sci. Rep. 6, 37311; doi: 10.1038/srep37311 (2016).
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We thank Heidi Thelen and Juliane Hancke for excellent technical assistance; the Leptin, Schnetz and Riemer labs for sharing equipment; members of the Gehring lab for useful discussions; Caroline Hoffmann for construction of SURF6 and PDRG1 reporters. This research was funded by grants from the Deutsche Forschungsgemeinschaft (GE2014/4-1) to N.H.G.
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Nature Communications (2017)