Figure 3 : Lymphoid organs and tumor microenvironment (TM) have lowered populations of myeloid cells and Treg cells in ligand-conjugates treated mice.

From: Tropomyosin Receptor Kinase C Targeted Delivery of a Peptidomimetic Ligand-Photosensitizer Conjugate Induces Antitumor Immune Responses Following Photodynamic Therapy

Figure 3

4T1 tumor bearing mice were randomly divided into four treatment groups (saline, I2-BODIPY, YIYI-I2-BODIPY and IYIY-I2-BODIPY) and compounds were administrated via tail vein respectively. Mice were then sacrificed at 2 h and 24 h post compound administration. At scheduled time points post compound administration, mice were sacrificed, followed by isolation of TDLN, spleen and tumor tissues. Immuno-phenotyping of (a) G-MDSC, (b) Treg and (c) neutrophil cells was conducted using surface staining of fluorescence conjugated antibodies and quantified using flow cytometry. Data represent mean ± SEM with minimum of four mice per group. *p < 0.05, ***p < 0.001 vs I2-BODIPY using one-way ANOVA (Dunnett’s test).