Figure 4 : Quantification of deformation of two non-cancerous and three cancer cell types on P4G4 surface.

From: A high throughput approach for analysis of cell nuclear deformability at single cell level

Figure 4

(a) Nuclei of the different cell types were categorised according to the regions of CV and R plot. L929 and hOB (non-cancerous cell types tested) had the highest number of nuclei in R1-R2, while SH-SY5Y, MCF7 and Saos-2 (cancerous cell lines) had the highest number of nuclei in R3-R5. Their corresponding deformation scores were L929: 2.17, hOB: 2.54, Saos-2: 4.02, MCF-7: 3.90 and SH-SY5Y: 3.55. (b) Comparison of deformation scores of the cells tested on unpatterned control and on P4G4 surfaces. Results show that all cell types except SH-SY5Y had deformation scores lower than 3 on the unpatterned control surface. On the other hand, non-cancerous cell types had deformation scores <3 while cancerous cell lines (MCF7, Saos-2, SH-SY5Y) had scores >3. (c–g) P4G4 surface was tested with 5 different cells: fibroblasts (L929), primary bone derived cells (hOB), osteosarcoma cells (Saos-2), neuroblastoma cells (SH-SY5Y), and breast cancer cells (MCF7). SEM images (Scale bars: 30 μm. Stains: OsO4) (c1-g1). CLSM images and z-stacks (Scale bars: 10 μm. Stains: Green: Nucleus/, DRAQ5, Red: actin cytoskeleton/Alexa Fluor 532-Phalloidin) (c2-g2).