Figure 6 : LEDGINs alter the IN oligomer in the nucleus.

From: Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry

Figure 6

(A) LEDGINs inhibit the interaction between LEDGF/p75 and IN by binding to the IN oligomer. (B) Fluorescence intensity of VSV-G pseudotyped HIVIN-eGFP complexes in the cytoplasm (white) or nucleus (grey) of HeLaP4 WT cells treated with DMSO or LEDGINs (45 μM, CX14442) during infection. (C) Percentage nuclear IN-eGFP complexes in HeLaP4 WT cells treated with DMSO or LEDGINs (45 μM, CX14442) during infection. (D) Mean FRET ratios of VSV-G pseudotyped HIVIN-mTFP1+IN-mVenus complexes in the cytoplasm (white) or nucleus (grey) of LEDGF/p75KD cells treated with DMSO or LEDGINs (45 μM, CX14442) during infection. Unless explicitly stated otherwise, the intensity and FRET ratios in the cytoplasm and nucleus were statistically different (p-value < 0.001). (**p-value < 0.01 and n.s. = not significant).