Figure 5 : The nuclear pore acts as a molecular filter, impeding the nuclear import of LEDGIN pre-treated viral particles.

From: Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry

Figure 5

(A) Fluorescence intensity of VSV-G pseudotyped HIV complexes (HIVIN-eGFP) in the cytoplasm (white) or nucleus (grey) of cells treated with DMSO, RAL (0.6 μM) or EVG (0.2 μM) during infection. (B) Fluorescence intensity of single IN-eGFP complexes in the cytoplasm (white circles) and nucleus (grey circles) at different time points post infection (0–24h). (C) Nuclear import of particles produced in the presence of DMSO or LEDGINs (1.2 μM, CX14442) is hampered. (D) Fluorescence intensity of viral particles and viral complexes localized in the cytoplasm or nucleus of HeLaP4 WT cells infected with VSV-G pseudotyped HIVIN-eGFP particles produced in the presence of DMSO (white) or LEDGINs (1.2 μM, CX14442) (grey). (E) FRET ratios of viral particles and viral complexes localized in the cytoplasm or nucleus in HeLaP4 WT cells infected with VSV-G pseudotyped HIVIN-mTFP1+IN-mVenus particles produced in the presence of DMSO (white) or LEDGINs (1.2 μM, CX14442) (grey). (F) Percentage nuclear complexes (HIVIN-eGFP) in HeLaP4 cells infected with VSV-G pseudotyped particles produced in the presence of DMSO or LEDGINs (1.2 μM, CX14442). Unless explicitly stated otherwise, the intensity and FRET ratios in the cytoplasm and nucleus were statistically different (p-value < 0.001). (***p-value < 0.001 and n.s. = not significant).