Figure 3 : Predicted Purkinje cell activity before and after VOR phase-reversal is consistent with the experimental data from GC-ΔKCC2 mice.

From: Modeled changes of cerebellar activity in mutant mice are predictive of their learning impairments

Figure 3

(a) Part of cerebellar circuitry shown in Fig. 1a; Red lightning bolts indicate loss of KCC2 from GCs. (b) Experimental and modeled eye movements in GC-ΔKCC2 mice as a function of training time of VOR phase-reversal training. Gain values (top panel) are normalized to the initial gain. Experimental data represent averages with SEM (green shaded area) and SD (grey shaded area) of the GC-ΔKCC2 mice. Grey dotted line indicates the values of the littermate controls. Modeled changes are displayed for both gain and phase with SD (red line). (c) Linear regression plots displaying correlation between modeled and experimental data. (d) Representative PSTHs of floccular VA PC cells depict SS and CS modulation in GC-ΔKCC2 mice before and after the VOR-reversal training during vestibular stimulation (0.6 Hz) (top and bottom panels, respectively). (e) Polar plots of SS (left) and CS (right) modulation before and after learning in GC-ΔKCC2 mice (lighter and darker color, respectively) reveal increase in the modulation amplitude following learning. Each dot represents a single cell. Phase of the modeled SS and CS are indicated with the arrows. (f) As predicted by the model SS modulation in GC-ΔKCC2 mice was much lower than that of the wild type before learning and increased after learning. Error bars denote SD; *denote p < 0.05; **denote p < 0.001. (g) SS activity displayed as a function of time in the cycle in the model with increased GC excitability (light green, initial value before learning; dark green, after training) and in controls (grey, initial value before learning; black, after training). The model predicts both the lowered modulation in naïve GC-ΔKCC2 mice and an increase in the modulation following the training.