Figure 2 : Experimental and modeled phase-reversal in control mice.

From: Modeled changes of cerebellar activity in mutant mice are predictive of their learning impairments

Figure 2

(a) Experimental and modeled eye movements in control mice as a function of training time of VOR phase-reversal (training is done in the light and eye measurement is done in the dark during VOR at 0.6 Hz). Gain values (left panel) are normalized to the initial gain. Experimental data represent averages with SEM (dark area) and SD (light grey area) of all control mice used in this study. Modeled changes are displayed for both gain and phase with SD (red line). (b) Linear regression plots displaying correlation between modeled and experimental data. (c) Example cell of an in vivo extracellular recording from floccular vertical axis (VA) PCs obtained during vestibular stimulation (0.6 Hz) in the dark before and after the VOR-reversal training in wild type mice (top, grey and bottom, black panels, respectively) plotted as peri-stimulus time histograms (PSTH). Green arrows indicate the depth of the modulation in the trained animals (peak to peak, marked with a green dotted line). (d) Polar plots of SS (top) and CS (bottom) modulation before and after learning in control mice (grey and black, respectively). Phase of the modeled SS and CS are indicated with arrows. The amplitude of modulation is depicted by the radius (green asterisk; calculated by subtracting the trough of SS from the peak of the SS activity) and the phase of modulation is indicated by the angle. Each dot represents a single cell. (e) As predicted by the model (red) the SS modulation was significantly increased following learning in the control mice. There was also a small but significant increase in the SS firing frequency following the training. Error bars denote SD; *denote p < 0.05; **denote p < 0.001. (f) Modeled PC SS activity as a function of time in the cycle in wild type mice (grey, initial value before learning; black, after training). SS activity produced by the model was normalized to the mean average firing frequency of the PC in control mice. Green arrows indicate the depth of the modulation in the trained animals (peak to peak, marked with a green dotted line).