Phenotypic characterization of the ∆HTL, ade2, yps11 and vpk1 strains.
(A) Drop test of each strain engineered on YPD plates at pH5.5 and 7.5 supplemented in NaCl (500 mM), ZnCl2 (10 mM), CaCl2 (100 mM) or caffeine (7.5 mM). Plates were stored for 1 day at 30 or 37 °C except for YPD plates supplemented with ZnCl2 stored for 3 days. (B) Average generation time of ∆HTL, ade2, yps11 and vpk1 strains monitored during growth in SCGlc 2% liquid medium. Numbers are mean generation time for each strain. n = 3, SEM are shown.