Figure 4 | Scientific Reports

Figure 4

From: The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability

Figure 4

EBLN1 deficiency leads to microtubule and centrosomal defects.

(A) Left panel; representative immunofluorescence images for γtubulin (centrosomes), αtubulin (microtubules) and merged images with DAPI (nucleus), in interphase U2OS cells transfected with either control or two individual EBLN1 siRNA. The white arrows on the αtubulin images highlight the position of the centrosome from which a microtubule array should emanate. Right panel; quantification of organised microtubule (MT) arrays in interphase U2OS cells treated with either control or EBLN1 siRNA as indicated. Data shown represents the mean from three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells). (B) Upper panel shows representative merged immunofluorescence images for γtubulin, αtubulin and DAPI at the indicated times points post release from ice treatment. The graph below shows mean percentage of control and EBLN1 siRNA transfected U2OS cells exhibiting an organised microtubule array. Data shown represents the mean from at least three independent experiments with associated SEMs (**p ≤ 0.01 compared to control siRNA cells). (C) Left panel shows representative merged immunofluorescence images for γtubulin and DAPI in U2OS cells transfected with the indicated siRNA. The white arrows highlight the centrosomes that are shown in the enlarged image below. Right panel shows the mean percentage of cells exhibiting split centrosomes in control and EBLN1 siRNA transfected (>2 microns apart). Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells).

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