Figure 2 | Scientific Reports

Figure 2

From: The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability

Figure 2

EBLN1-depleted cells fail to adequately repair exogenously induced DNA damage.

(A) Left panel shows representative immunofluorescence images for phosphorylated ATM (pATM Ser1981) in HeLa cells transfected with either control of two individual EBLN1 siRNA at the indicated times post 3Gy IR. The graph on the right shows mean percentage of pATM foci positive cells in control and EBLN1 siRNA transfected cells. Data shown represents the mean from at least three independent experiments with associated SEMs (**p ≤ 0.01 compared to control siRNA cells). (B) Same as (A), but for γH2AX positive cells. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells). (C) Same as (B), but for 53BP1 foci positive cells. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells). (D) Clonogenic survival curves for control and EBLN1 siRNA-transfected HeLa cells treated with the indicated doses of IR. Data shown represents the mean surviving fractions with their respective SEMs calculated from at least three independent experiments. Comparable data was obtained in MRC5A cells (Supplementary Figure S1B). (E) FACS-based quantification (PI staining) of G2/M cells in control and EBLN1 siRNA-transfected HeLa cell populations at the indicated times post 3Gy IR. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells). (F) Quantification of micronuclei in HeLa cells, as assessed by DAPI immunofluorescence staining, forty-eight hours post-transfection with either control or EBLN1 siRNA. Data shown represents the mean from at least three independent experiments with associated SEMs (*p ≤ 0.05 and **p ≤ 0.01 compared to control siRNA cells).

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