The properties of the gut microbial community are altered by SF treatment.
(a) PCoA plot demonstrates structural changes in microbial communities over the period of 7 weeks, introduced by SF exposure followed by 2 weeks of sleep recovery. The major clusters (a–d) of individual points (averaged microbiota per treatment per day) were outlined by 68% similarity between data points (Bray-Curtis index), ANOSIM test confirms separation (p = 0.001). The data points are labelled by the week of experiment (0 week - a week of acclimation; 1–4 weeks - SF exposure, 5–6 week – recovery). (b) Differentially abundant bacterial phyla and (c) families in SF and CTL samples at weeks 2–4 (METASTATS, p < 0.02). (d,e) The abundance of Lachnospiraceae and Lactobacillaceae family members is different between SF and CTL groups at weeks 2–4 (W2–4) (*p < 0.002), but not at the initiation of treatment (INI) and at the end of recovery period (2R). Boxplots represent median and 25–75 percentiles.