Figure 3 | Scientific Reports

Figure 3

From: Chronic stress prior to pregnancy potentiated long-lasting postpartum depressive-like behavior, regulated by Akt-mTOR signaling in the hippocampus

Figure 3

Behavioral effects by acute ketamine or chronic fluoxetine treatment in SP mice and alternations of Akt/mTOR signaling.

(A,B) Effects on the SPT (A) or immobility time in FST (B) 24 hours after an acute ketamine or 3-week fluoxetine treatment in SP mice. n = 5–7/group.***p < 0.001, compared to control, ###p < 0.001, compared to vehicle.(C,D) Latency to eat (C) and food consumption (D) in NSF test at 24 hours after an acute administration of ketamine (ket, 30 mg/kg, i.p.) and the last chronic fluoxetine (flx, 18 mg/kg/day x21d, s.c.) treatment in SP mice. n = 5–7/group. *p < 0.05, ***p < 0.001, compared to control, #p < 0.05, ###p < 0.001, compared to vehicle. (E,F) Effects of pharmacological inhibition of mTOR using rapamysin (rapa) on the antidepressant effects of ketamine (ket) on SPT (E) and FST (F) in SP group mice.n = 8/group.***p < 0.001, compared to non-drug control, ###p < 0.001, compared to group of ket + veh. (G) Hippocampal protein expressions of NR1, Akt, mTOR, p70s6k, 4EBP1 and GluR1 at 24 hours after an acute administration of ketamine in SP mice, n = 5/group. Data represent mean ± SEM.*p < 0.05, ***p < 0.001, compared to control; #p < 0.05, ###p < 0.001, compared to vehicle.

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