Figure 3

From: A general solution for opening double-stranded DNA for isothermal amplification

Figure 3

(A) Schematic of the process of RecA mediated ligation of two ssDNA oligonucleotides using double-stranded DNA as template. Firstly, RecA bind with two ssDNA oligonucleotides to form nucleoprotein complex respectively to recognize and unwound double-strand template homologously. Then, the two oligonucleotides annealed with complementary strand in juxtaposition and are ligated by T4 DNA ligase. (B) PAGE analysis of RecA mediated ligation reaction: Lane 1, the reaction was conducted in presence of dATP. Lane 2, RecA was not included. Lane 3, RecA was included while the double-stranded DNA template was removed. Lane 4 and 5, 32P-labeled 108 nt and 54 nt oligonucleotide as markers.