The application of high-resolution genetic data has revealed that oceanographic connectivity in marine species with planktonic larvae can be surprisingly limited, even in the absence of major barriers to dispersal. Australia’s southern coast represents a particularly interesting system for studying planktonic larval dispersal, as the hydrodynamic regime of the wide continental shelf has potential to facilitate onshore retention of larvae. We used a seascape genetics approach (the joint analysis of genetic data and oceanographic connectivity simulations) to assess population genetic structure and self-recruitment in a broadcast-spawning marine gastropod that exists as a single meta-population throughout its temperate Australian range. Levels of self-recruitment were surprisingly high and oceanographic connectivity simulations indicated that this was a result of low-velocity nearshore currents promoting the retention of planktonic larvae in the vicinity of natal sites. Even though the model applied here is comparatively simple and assumes that the dispersal of planktonic larvae is passive, we find that oceanography alone is sufficient to explain the high levels of genetic structure and self-recruitment. Our study contributes to growing evidence that sophisticated larval behaviour is not a prerequisite for larval retention in the nearshore region in planktonic-developing species.
Many coastal marine species have a two-phase life-cycle in which adults are sessile or sedentary, with dispersal instead facilitated by pelagic propagules such as eggs and planktonic larvae1,2. Given the small size of these ‘dispersive’ propagules, it has traditionally been assumed that they are transported passively by ocean currents3 and that the majority of larvae that settle in a particular area may have originated elsewhere in a species’ range4,5. Recent studies, however, have suggested that planktonic dispersal may not be as passive as previously assumed, but rather that propagule behaviour can promote larval retention in the vicinity of parental habitats6,7.
Direct measurements of propagule dispersal are almost invariably difficult to obtain8, so various indirect approaches may be required to help elucidate larval movement patterns. The application of high-resolution genetic data, such as polymorphic microsatellites, has confirmed that connectivity between populations of marine species with high theoretical dispersal potential is often lower than expected9,10,11,12. For example, while positive correlations among genetic and geographic distance have been viewed as a defining feature of low-dispersal species, such as those that lack a planktonic dispersal phase13,14, there is increasing evidence that geographic distance can also reduce connectivity in planktonic dispersers11,15,16,17.
In recent years, seascape genetics (i.e., the joint analysis of realized dispersal based on genetic data and potential larval dispersal based on advection connectivity simulations) has proven to be particularly powerful in helping to identify factors that limit connectivity in the oceans11,18,19. In the present study, we used a seascape genetic approach to determine how oceanography affects genetic connectivity in a widespread temperate Australian marine invertebrate. The study species, Siphonaria diemenensis (Quoy & Gaimard, 1833) is a common rocky shore limpet that occurs throughout southern and eastern temperate Australia20. It has a high fecundity21 and a planktonic larval dispersal phase22 and unlike many other coastal invertebrates, it is not genetically subdivided into regional genetic units whose ranges are linked to the region’s biogeographic provinces23,24. These features make S. diemenensis a particularly suitable model for studying the effect of ocean circulation on the connectivity of rocky shore fauna with theoretically high dispersal potential.
Although southern Australia is dominated by three ocean currents (the Leeuwin Current, the South Australian Current and the Zeehan Current), which together can potentially connect the region’s entire fauna over a distance > 5000 km25, realised dispersal is often surprisingly limited10,11. This paradox has sometimes been attributed to the fact that southern Australia’s offshore currents are relatively weak10. In contrast, recent seascape genetic analyses suggest that the lack of larval dispersal may be attributable to on-shelf circulation11. The continental shelf in some regions may be up to 100 km wide and it is likely that most larvae never reach the shelf-edge boundary currents11. In the present study, we identify particularly high levels of self-recruitment and demonstrate that near-shore oceanographic constraints play key roles in limiting the spread of passively-dispersing particles.
Tissue samples from the foot of 714 individuals were collected from 16 localities (Fig. 1, Table S1) during 2011 and 2012, with sample sizes ranging from 29 to 48 (mean: 44.6 individuals). Samples from each locality generally included multiple size classes (e.g. shell length ranged from 5 to 28 mm). This strategy was used to avoid the inclusion of related individuals in the analysis and to include multiple cohorts, thus reducing issues associated with chaotic genetic patchiness due to stochastic recruitment26. Samples were stored in 99% ethanol, for no more than 1 month, until DNA was extracted using a salting-out protocol27. Thirteen microsatellites developed for S. diemenensis were amplified as described in28, namely Side01, Side03, Side04, Side05, Side07, Side09, Side12, Side13, Side15, Side17, Side18, Side19 and Side20. Fragments were separated on an ABI 3730 Genetic Analyser (Applied Biosystems) and alleles were scored using GENEMAPPER v. 3.0 (Applied Biosystems). Tests for genotyping errors, null-alleles and large allele drop-out were performed with MICRO-CHECKER29 and tests for departures from Hardy-Weinberg equilibrium and linkage disequilibrum were done using Arlequin v. 18.104.22.168. Arlequin was also used to calculate observed and expected heterozygosity at each site and for the complete data set, to identify the number of polymorphic loci and to report allele size ranges.
Genetic structure and spatial analyses
To measure genetic structure between sites, we calculated pairwise G″ST in GenAlEx 6.531. This statistic is a nearly unbiased estimator of G′ST32 that should be used when sample sizes are small, making it suitable for pairwise comparisons33. For microsatellite data, it is preferable to the more commonly used FST34 because it corrects for the maximum value possible when no alleles are shared between populations, which for microsatellites tends to be below the theoretical maximum of 1.033. For comparison, we also calculated FST. Significance of both statistics at α = 0.05 was assessed by running 999 permutations in GenAlEx and the B-Y false discovery rate method35 was applied to account for multiple comparisons.
Geographic distances between sites were measured as minimum coastline distances in ARCMAP 10.1 (Environmental Systems Research Institute, Redlands, CA). GenAlEx was used to perform a paired Mantel test36,37 for a matrix comprising G″ST values and one comprising geographic distances and to construct a regression plot. Significance for the Mantel test was based on 999 permutations. GenAlEx was also used to perform a multilocus spatial autocorrelation analysis and to calculate within-population relatedness. Both methods are useful to determine whether larval recruitment occurs mostly in close proximity to the parent site, in which case the spatial autocorrelation coefficient r38 is expected to be greater than expected under a null hypothesis of no spatial genetic structure at smaller distance classes. This is because individuals that were collected in close geographic proximity to each other are expected to be more closely related to each other than they are to individuals from more distant sites. Statistical significance was based on 999 permutations to estimate 95% confidence intervals around the null hypothesis and 1000 bootstrap replications to estimate the 95% confidence interval around r. Spatial genetic structure is present when the value of r is beyond the confidence interval of the null hypothesis and when the confidence interval of r does not overlap with zero. After exploring a number of distance class sizes, we presented an autocorrelation correlogram with a distance class size of 120 km, as this resulted in particularly good resolution for our sampling design.
As an independent and complimentary means of assessing levels of self-recruitment, relatedness or kinship indices have proven to be useful because individuals from the same site are more likely to be closely related to each other than they are to individuals from other sites39. We calculated within-population relatedness using the relatedness coefficient r40 for pairs of individuals from the same site (referred to here as rQG to distinguish it from the spatial autocorrelation statistic). Genotypes from all sites were permuted 999 times to calculate 95% confidence intervals for the range of rQG expected under conditions of spatial genetic homogeneity.
Relationships between empirical genetic data and oceanography
The effect of ocean circulation on genetic connectivity was assessed using oceanographic connectivity simulations. These were performed on OFES hydrodynamic data41 at 5 m depth with the Connectivity Modeling System42 using two models that take into consideration information on the species’ spawning period43. The first (Model 1) assumed a short peak spawning season (October 1–31) and negligible recruitment for other months, while the second (Model 2) assumed that spawning occurred over a period of 3 months (September 1 to November 30). In both cases, particles were released every hour from each of the 16 sites for the years 1980 to 2009 and were advected for 30 days. Unlike in the closely related S. denticulata, where there is a lag of 2–3 months between hatching and settlement44, no lag was observed in S. diemenensis43, suggesting that this species’ planktonic larval duration is shorter and 1 month larval duration adequate. For Model 1, 15 particles were released every hour and 5 particles were released every hour for Model 2 (as Model 2 has a three times longer spawning season), resulting a release of ~5.3 million particles for both models. We further differentiated between 1 spawning cycle (hereafter referred to as ‘1 generation’, given a generation time of 1 year) and 5 spawning cycles (hereafter ‘5 generations’) in which a particular individual could take part. For the model that included 5 spawning cycles, following the first spawning event the number of both locally retained and imported particles was determined for each site and used to determine the particles released from that locality for the subsequent spawning cycle. These patterns of particle dispersal were averaged across the 30 years (i.e. from 1980 to 2009). See11 for additional details on the oceanographic connectivity simulations.
Relationships between G″ST values and oceanographic connectivity matrices was assessed using Mantel tests, as described earlier and Multiple Regression on Distance Matrices (MRDM45,46). The Mantel tests were performed between the matrix of G″ST values and one of four oceanographic connectivity matrices (Model 1 for 1 generation; Model 1 for 5 generations; Model 2 for 1 generation; and Model 2 for 5 generations). As the value of G″ST for each pair of sites is a consequence of both immigration and emigration, a single oceanographic connectivity value was calculated for each pair of sites as the sum of the number of settlers released from a particular site that reached the other site and the number of settlers received from that site. In addition, as the inferred number of settlers differed by several orders of magnitude, each estimate was corrected for the Mantel test by taking its natural logarithm.
As Mantel tests have been criticised for having an inflated Type I error rate47, MRDM was used as an alternative approach to corroborate the results from the Mantel tests. MRDM is a multivariate method that uses multiple regression to simultaneously test for correlations between a dependent variable and one or more explanatory variables. Our ln-transformed explanatory variables had high levels of collinearity, with VIF (Variance Inflation Factors) for 3 out of 4 advection connectivity models being >5.0 (range: 3.9 to 10.5) when all models were analysed together (5 is considered to be the maximum acceptable VIF48). Because of this, we analysed data sets with only 1 or 2 explanatory variables. In the latter cases, one of the four advection connectivity models was simultaneously analysed with geographic distance, as VIF values were lower for these combinations (range: 1.7 – 2.7), with the exception of the combination that included Model 1 with 5 generations, which had a much higher VIF value (6.9) and was excluded. MRDM analyses were run in the R package ECODIST49 and significance was based on 10 000 permutations.
To determine how well the simulated oceanographic data explained the self-recruitment inferred with the genetic data, Spearman rank correlations50 in SigmaStat 1.0 (Systat Software, San Jose, CA) were used to assess whether mean relatedness at specific sites was correlated with the number of particles that returned to the release site. We also used this test to assess how particles reaching the continental shelf (i.e. water with a depth of >100 m) affected self-recruitment and larval loss.
All microsatellite loci were variable in samples from each site. The number of alleles per locus ranged from 5 (locus Side18) to 31 (Side20) for the combined data and for individual sampled sites, it ranged from 2 (Side18 at sites 2 and 13) to 23 (Side09 at site 5). The mean number of alleles across all loci was similar at the different sampled sites and ranged from 11.2 to 12.7 (Table S1). Observed and expected heterozygosity were high (Table S1), with an average of 0.79 and 0.81, respectively. There was no evidence for null alleles, sequencing errors or large allele dropout. All loci were thus considered suitable for inclusion in population genetic analyses.
Of the G″ST values calculated for 120 pairs of sites (Table 1), 85 were significant after correction for multiple tests (P < 0.015: 22 comparisons, P < 0.003: 63 comparisons). Uncorrected FST values were lower (Table S2), but the number of pairwise comparisons that were significant was very similar (P < 0.015: 18 comparisons, P < 0.003: 66 comparisons).
A Mantel test performed on a matrix of pairwise geographic distances and a matrix of pairwise values of G″ST was highly significant (Rxy = 0.385, P = 0.002), indicating that genetic structure can be described by a pattern of isolation by geographic distance. This was also evident in the positive correlation identified in the corresponding regression plot (Fig. S1). A spatial autocorrelation correlogram revealed that this relationship between genetic and geographic distances was primarily due to self-recruitment, with individuals from the same site being particularly closely related to each other (Fig. 2). The autocorrelation parameter r then decreased and from 360 km onwards, there was no longer any clear trend in spatial patterns.
Mean pairwise relatedness within sites (Fig. 3) confirmed that the highly significant positive spatial autocorrelation at the lowest distance class size (Fig. 2) was explained by high levels of self-recruitment, which was evident at seven sites. The relatedness coefficient rQG was significantly greater than expected under conditions of panmixia at the five westernmost sites (and particularly high at the two sites located within the South Australian gulfs), but also at sites 8 and 12.
Oceanographic connectivity simulations indicated that the high levels of self-recruitment shown in Figs 2 and 3 were largely a result of larval retention in the nearshore area (Figs 1 and 4, Fig. S2; Supplementary Materials: Animation). The net direction of surface flow was in most cases shoreward, making it very unlikely that advected particles would reach the region’s boundary currents, with eastern Tasmania being the only clear exception (Fig. 1). As a result, most advected particles settled at the release site and recruitment beyond neighbouring sites was limited. This trend was particularly strong in the western portion of the species’ range, where high levels of self-recruitment were inferred for sites within the South Australian gulfs (sites 4 and 5), but also at more exposed sites (3 and 6). Mantel tests on matrices of ln-transformed connectivity simulated for a single generation against the genetic structure statistic G″ST revealed a significant negative correlation for both the short spawning model, Model 1 (Rxy = −0.50, P = 0.006) and the long spawning model, Model 2 (Rxy = −0.53, P = 0.003). For the 5 generation simulations, the relationship was non-significant for Model 1 (Rxy = −0.34, P = 0.07) but remained significant for the more realistic Model 2 (Rxy = −0.477, P = 0.005). The two matrices have an inverse relationship because the more strongly two sites are connected by migration, the lower G″ST is for that pair of sites (Fig. S3).
The results of the MRDM analyses were congruent with those of the Mantel tests. Tests were significant for all single explanatory variables, with the exception of Model 1, 5 generations (C in Table S3). On the basis of F-tests, the best model was Model 2, 1 generation (D in Table S3). Analyses that included more than one explanatory variable were all significant, but F-statistics were intermediate.
Mean relatedness (Table S4) at each of the 16 sites was positively correlated with simulated self-recruitment (R = 0.56, P = 0.02). A positive correlation was also found between the number of simulated particles that reached the shelf edge (Table S3) and those that failed to return to the coast to settle (R = 0.64, P < 0.01) and there was a negative correlation between those particles that reached the continental shelf and the level of self-recruitment (R = −0.71, P < 0.01). This indicates that particles that became entrained in the region’s shelf-edge boundary currents were not only less likely to return to the parent habitat, but also had lower recruitment success.
Evaluating how planktonic larvae recruit back into their source populations and how much gene flow takes place between different populations, is not only critical for understanding the population dynamics of coastal species, but also to manage and conserve such populations1,51. In coastal populations, which tend to be characterized by weak signals of population differentiation, a pattern of isolation by geographic distance is often the only indication for a departure from the assumption of panmixia51. As discussed below, the joint analysis of genetic and oceanographic information represents a powerful means of elucidating factors underlying limited dispersal.
In the present study, a simple advection connectivity model, which treated larvae as passive particles and assumed that they remained in the surface layer throughout their dispersal phase, was adequate to explain high levels of genetic structure and self-recruitment. The considerable differences in these estimates at specific sites in the sampling region are readily explained by differences in how oceanographic regimes influence larval dispersal. Self-recruitment was particularly high in the west of the sampled range, as indicated by the high proportion of simulated particles that settled at their natal site and the high levels of relatedness among individuals from almost half of the study sites. In contrast, mean relatedness was low at most eastern sites, where the larger number of simulated particles reaching the continental shelf was positively correlated with greater larval loss. This difference between regions is not simply due to the presence of large bays in the west, where larval retention can be expected to be high52, as several of the more exposed sites also had circulation patterns that promoted self-recruitment. These results support the idea that in areas of South Australia where the continental shelf is particularly wide, planktonic propagules are primarily affected by local wind-driven circulation and coastal trapped waves53,54. The findings of the seascape genetic approach employed here are consistent with results of previous modelling work for a teleost fish, which suggested that recruitment in South Australia occurs at a highly localized scale, even when larval duration exceeds 100 days55. In contrast, at sites where the continental shelf is narrow and larvae are more likely to be advected by high velocity currents (e.g. in eastern and western Tasmania), self-recruitment is considerably less important. Studies conducted elsewhere confirm that a wide continental shelf can significantly reduce the influence of high-velocity offshore currents56,57 whereas long-distance dispersal by means of shelf-edge currents is more likely when the continental shelf is narrow58. In species that actively migrate to specific areas to spawn, the spawning areas are typically located where the shelf is particularly wide, which minimizes larval loss56. However, shelf width per se does not necessarily determine high levels of self-recruitment. Depending on local conditions, larval concentrations may decrease significantly from the coast to the shelf edge59, or on-shelf circulation may at least temporarily facilitate dispersal over the entire continental shelf 57.
The strong correlation between genetic structure and oceanography identified in this study is likely to apply more widely to most southern Australia’s mollusks that disperse by means of planktotrophic veliger larvae. Unlike many fish and crustaceans, whose late-stage larval often have considerable swimming ability and sensory competency that may reduce the influence of ocean currents on their dispersal60,61,62, veligers have comparatively low swimming abilities and it is often assumed that they disperse like passive particles over large distances63,64,65. In addition, even though settlement behaviour can be quite sophisticated66, veliger behaviour during the dispersal phase tends to be limited to “reverse diel migration” (rising to the surface at night and sinking during daytime), which serves to avoid predators and exposure to ultraviolet radiation67. This is very different from the advection avoidance achieved in some fish larvae through a benthic lifestyle68. For that reason, veliger behaviour is less likely to promote nearshore retention in the presence of strong surface flows, which may explain why the dispersal of veliger larvae can be accurately predicted exclusively on the basis of hydrographic data65. Although a model that assumes passive dispersal of veligers is clearly a simplification and a more sophisticated model may result in stronger correlations between connectivity simulations and genetic data, such a model is unlikely to challenge the finding of the present study that self-recruitment is driven by the hydrodynamic regime of the south Australian continental shelf.
In addition to assuming passive dispersal, other simplifications may have affected our simulations. First, we simulated larval dispersal from early September to the end of November43, yet S. diemenensis seems to be quite flexible in terms of when it spawns. Recruitment has also been reported between May and July69, when current flow along the continental shelf is strongest and continuous eastward shelf-edge flow develops that can theoretically disperse planktonic larvae from the Australian south-west coast all the way to Tasmania25,70. The fact that this current flow occurs far offshore, while most larvae remain in the nearshore region where its influence is much diminished71, suggests that its importance in connecting sites throughout the region is insignificant in areas where the continental shelf is wide.
Secondly, our simulations may also have been affected by the fact that there is no detailed information on the planktonic larval duration of S. diemenensis. While larval duration can be inferred indirectly on the basis of spawning and recruitment patterns43, it is possible that the 1-month larval duration used here is overly conservative. The eggs of S. diemenensis22 are only slightly smaller than those of the often co-distributed S. denticulata, whose larvae also hatch well developed and may remain pelagic for up to 10 weeks44. If one assumes that egg size is positively correlated with developmental stage at hatching, because congeners that hatch fully developed have the largest eggs21, then a ~2-month larval duration is feasible for S. diemenensis. This is also unlikely to significantly affect genetic structure because there is no clear difference in realised dispersal distances of species whose larvae are part of the plankton for a few days compared to those that remain pelagic for weeks72,73.
Our study contributes to the growing evidence that nearshore hydrodynamic processes can promote the retention of larvae close to parental habitats, even in ‘dispersive’ taxa11,74,75,76. On the wide continental shelf of South Australia, where the influence of the region’s shelf-edge boundary currents is minimal, complex larval behaviour is not necessary to explain high levels of self-recruitment. These findings have important implications for the design of marine reserves. The long-term persistence of populations within reserves is facilitated by a combination of self-recruitment and connectivity with other populations77. Our study supports the idea that, in the case of South Australian rocky shore fauna, an approach that relies on closely spaced networks of small reserves78 represents a more suitable management approach than the design of large but geographically distant reserves, because the majority of larvae will not disperse over greater distances55.
How to cite this article: Teske, P. R. et al. Oceanography promotes self-recruitment in a planktonic larval disperser. Sci. Rep. 6, 34205; doi: 10.1038/srep34205 (2016).
Swearer, S. E. et al. Evidence of self recruitment in demersal marine populations. Bull. Mar. Sci. 70, 251–271 (2002).
Lester, S. E., Gaines, S. D. & Kinlan, B. P. Reproduction on the edge: Large-scale patterns of individual performance in a marine invertebrate. Ecology 88, 2229–2239 (2007).
Williams, D. M., Wolanski, E. & Andrews, J. C. Transport mechanisms and the potential movement of planktonic larvae in the central region of the Great Barrier Reef. Coral Reefs 3, 229–236 (1984).
Roughgarden, J., Gaines, S. & Possingham, H. Recruitment dynamics in complex life cycles. Science (80-.). 241, 1460–1466 (1988).
Sale, P. F. In Habitat Structure (ed. Bell, S. S. ) 197–210 (Chapman and Hall, 1991).
Kingsford, M. J. et al. Sensory environments, larval abilities and local self-recruitment. Bulletin of Marine Science 70, 309–340 (2002).
Butler, M. J. IV, Paris, C. B., Goldstein, J. S., Matsuda, H. & Cowen, R. K. Behavior constrains the dispersal of long-lived spiny lobster larvae. Mar. Ecol. Prog. Ser. 422, 223–237 (2011).
Thorrold, S. R. et al. Quantifying larval retention and connectivity in marine populations with artificial and natural markers. Bulletin of Marine Science 70, 291–308 (2002).
Hellberg, M. E., Burton, R. S., Neigel, J. E. & Palumbi, S. R. Genetic assessment of connectivity among marine populations. Bulletin of Marine Science 70, 273–290 (2002).
Coleman, M. A., Feng, M., Roughan, M., Cetina-Heredia, P. & Connell, S. D. Temperate shelf water dispersal by Australian boundary currents: implications for population connectivity. Limnol. Oceanogr. Fluids Environ. 3, 295–309 (2013).
Teske, P. R., Sandoval-Castillo, J., van Sebille, E., Waters, J. & Beheregaray, L. B. On-shelf larval retention limits population connectivity in a coastal broadcast spawner. Mar. Ecol. Prog. Ser. 532, 1–12 (2015).
Banks, S. C. et al. Oceanic variability and coastal topography shape genetic structure in a long-dispersing sea urchin. Ecology 88, 3055–3064 (2007).
Kyle, C. J. & Boulding, E. G. Comparative population genetic structure of marine gastropods (Littorina spp.) with and without pelagic larval dispersal. Mar. Biol. 137, 835–845 (2000).
Teske, P. R. et al. Implications of life history for genetic structure and migration rates of southern African coastal invertebrates: planktonic, abbreviated and direct development. Mar. Biol. 152, 697–711 (2007).
Piggott, M. P., Banks, S. C., Tung, P. & Beheregaray, L. B. Genetic evidence for different scales of connectivity in a marine mollusc. Mar. Ecol. Prog. Ser. 365, 127–136 (2008).
Brown, L. D. Genetic variation and population structure in the blacklip abalone, Haliotis rubra. Aust. J. Mar. Freshw. Res. 42, 77–90 (1991).
Pogson, G. H., Taggart, C. T., Mesa, K. A. & Boutilier, R. G. Isolation by distance in the Atlantic cod, Gadus morhua, at large and small geographic scales. Evolution (N. Y). 55, 131–146 (2001).
Selkoe, K. A. et al. Taking the chaos out of genetic patchiness: seascape genetics reveals ecological and oceanographic drivers of genetic patterns in three temperate reef species. Mol. Ecol. 19, 3708–3726 (2010).
White, C. et al. Ocean currents help explain population genetic structure. Proc. R. Soc. B Biol. Sci. 277, 1685–94 (2010).
Colgan, D. J. & da Costa, P. Possible drivers of biodiversity generation in the Siphonaria of southeastern Australia. Mar. Biodivers. 43, 73–85 (2013).
Chambers, R. & McQuaid, C. A review of larval development in the intertidal limpet genus Siphonaria (Gastropoda: Pulmonata). J. Molluscan Stud. 60, 415–423 (1994).
Mapstone, G. M. Egg capsules and early development in Siphonaria diemenensis (Quoy & Gaimard, 1833) and Siphonaria baconi (Reeve, 1856). J. Malacol. Soc. Aust. 4, 85–92 (1978).
Colgan, D. J. Marine and estuarine phylogeography of the coasts of south-eastern Australia. Mar. Freshw. Res, doi: 10.1071/MF15106 (2015).
Teske, P. R., Sandoval-Castillo, J., Waters, J. & Beheregaray, L. B. An overview of temperate Australian marine phylogeography, with new evidence from high-dispersal gastropods. J. Biogeogr (2016).
Ridgway, K. R. & Condie, S. A. The 5500-km-long boundary flow off western and southern Australia. J. Geophys. Res. Ocean . 109, 2156–2202 (2004).
Hedgecock, D. & Pudovkin, A. I. Sweepstakes reproductive success in highly fecund marine fish and shellfish: A review and commentary. Bulletin of Marine Science 87, 971–1002 (2011).
Sunnucks, P. & Hales, D. F. Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae). Mol. Biol. Evol. 13, 510–524 (1996).
Sandoval-Castillo, J., Gardner, M. G. & Beheregaray, L. B. Isolation and characterization of microsatellite markers for the marine black nerite Nerita atramentosa: tools for assessment and design of marine protected areas. Conserv. Genet. Resour. 4, 625–627 (2012).
Van Oosterhout, C., Hutchinson, W. F., Wills, D. P. M. & Shipley, P. MICRO-CHECKER: Software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes 4, 535–538 (2004).
Excoffier, L. & Lischer, H. E. L. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 10, 564–567 (2010).
Peakall, R. & Smouse, P. E. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28, 2537–2539 (2012).
Hedrick, P. W. A standardized genetic differentiation measure. Evolution (N. Y). 59, 1633–1638 (2005).
Meirmans, P. G. & Hedrick, P. W. Assessing population structure: FST and related measures. Molecular Ecology Resources 11, 5–18 (2011).
Wright, S. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution (N. Y). 19, 395–420 (1965).
Benjamini, Y. & Yekutieli, D. The control of the false discovery rate in multiple testing under dependency. Ann. Stat. 29, 1165–1188 (2001).
Smouse, P. E. & Long, J. C. Matrix correlation analysis in anthropology and genetics. Am. J. Phys. Anthropol. 35, 187–213 (1992).
Smouse, P. E., Long, J. C. & Sokal, R. R. Multiple regression and correlation extensions of the Mantel test of matrix correspondence. Syst. Zool. 35, 627–632 (1986).
Smouse, P. E. & Peakall, R. Spatial autocorrelation analysis of individual multiallele and multilocus genetic structure. Heredity (Edinb). 82 (Pt 5), 561–573 (1999).
Iacchei, M. et al. Combined analyses of kinship and FST suggest potential drivers of chaotic genetic patchiness in high gene-flow populations. Mol. Ecol. 22, 3476–3494 (2013).
Queller, D. C. & Goodnight, K. F. Estimating relatedness using genetic markers. Evolution (N. Y). 43, 258–275 (1989).
Masumoto, Y. et al. A fifty-year eddy-resolving simulation of the world ocean: preliminary outcomes of OFES (OGCM for the Earth Simulator). J. Earth Simulator 1, 35–56 (2004).
Paris, C. B., Helgers, J., Van Sebille, E. & Srinivasan, A. Connectivity Modeling System: A probabilistic modeling tool for the multi-scale tracking of biotic and abiotic variability in the ocean. Environ. Model. Softw. 42, 47–54 (2013).
Parry, G. D. Life-history strategies of five species of intertidal limpet. (University of Melbourne, 1977).
Creese, R. Reproductive cycles and fecundities of two species of Siphonaria (Mollusca: Pulmonata) in south-eastern Australia. Aust. J. Mar. Freshw. Res. 31, 37–47 (1980).
Manly, B. F. J. Randomization and regression methods for testing for associations with geographical, environmental and biological distances between populations. Res. Popul. Ecol. (Kyoto). 28, 201–218 (1986).
Legendre, P., Lapointe, F.-J. & Casgrain, P. Modeling brain evolution from behavior: a permutational regression approach. Evolution (N. Y). 48, 1487–1499 (1994).
Guillot, G. & Rousset, F. Dismantling the Mantel tests. Methods Ecol. Evol. 4, 336–344 (2013).
Rogerson, P. Statistical methods for geography. (Sage, 2001).
Goslee, S. & Urban, D. The ecodist Package: Dissimilarity-based functions for ecological analysis. J. Stat. Softw. 22, 1–19 (2007).
Spearman, C. The proof and measurement of association between two things. Am. J. Psychol. 15, 72–101 (1904).
Purcell, J. F. H., Cowen, R. K., Hughes, C. R. & Williams, D. a. Weak genetic structure indicates strong dispersal limits: a tale of two coral reef fish. Proc. R. Soc. B Biol. Sci. 273, 1483–90 (2006).
McShane, P. E., Black, K. P. & Smith, M. G. Recruitment processes in Haliotis rubra (Mollusca: Gastropoda) and regional hydrodynamics in southeastern Australia imply localized dispersal of larvae. J. Exp. Mar. Bio. Ecol. 124, 175–203 (1988).
Middleton, J. F. & Black, K. P. The low frequency circulation in and around Bass Strait: a numerical study. Cont. Shelf Res. 14, 1495–1521 (1994).
Teixeira, C. E. P. Ocean dynamics of Spencer Gulf: a numerical study. (University of New South Wales, 2010).
Fowler, A. J., McLeay, L. & Short, D. A. Spatial variation in size and age structures and reproductive characteristics of the King George whiting (Percoidei: Sillaginidae) in South Australian waters. Mar. Freshw. Res. 51, 11–22 (2000).
Hutchings, L. et al. Spawning on the edge: Spawning grounds and nursery areas around the southern African coastline. In Marine and Freshwater Research 53, 307–318 (2002).
Epifanio, C. E. & Garvine, R. W. Larval transport on the Atlantic continental shelf of North America: a review. Estuar. Coast. Shelf Sci. 52, 51–77 (2001).
Hare, J. A. et al. Routes and rates of larval fish transport from the southeast to the northeast United States continental shelf. Limnol. Oceanogr. 47, 1774–1789 (2002).
Queiroga, H. Distribution and drift of the crab Carcinus maenas (L) (Decapoda, Portunidae) larvae over the continental shelf off northern Portugal in April 1991. J. Plankton Res. 18, 1981–2000 (1996).
Fisher, R., Bellwood, D. R. & Job, S. Development of swimming abilities in reef fish larvae. Mar. Ecol. Ser. 202, 163–173 (2000).
Lecchini, D., Shima, J., Banaigs, B. & Galzin, R. Larval sensory abilities and mechanisms of habitat selection of a coral reef fish during settlement. Oecologia 143, 326–334 (2005).
Kough, A. S., Paris, C. B. & Staaterman, E. In situ swimming and orientation behavior of spiny lobster (Panulirus argus) postlarvae. Mar. Ecol. Prog. Ser. 504, 207–219 (2014).
Bayne, B. L. In Marine mussels: their ecology and physiology (ed. Bayne, B. L. ) 81–120 (Cambridge University Press, 1976).
Widdows, J. Physiological ecology of mussel larvae. Aquaculture 94, 147–163 (1991).
McQuaid, C. D. & Phillips, T. E. Limited wind-driven dispersal of intertidal mussel larvae: In situ evidence from the plankton and the spread of the invasive species Mytilus galloprovincialis in South Africa. Mar. Ecol. Prog. Ser. 201, 211–220 (2000).
Barlow, L. A. Electrophysiological and behavioral responses of larvae of the red abalone (Haliotis rufescens) to settlement-inducing substances. Bull. Mar. Sci. 46, 537–554 (1990).
Becker, B. J., Levin, L. A., Fodrie, F. J. & McMillan, P. A. Complex larval connectivity patterns among marine invertebrate populations. Proc. Natl. Acad. Sci. 104, 3267–3272 (2007).
Tilney, R. L., Nelson, G., Radloff, S. E. & Buxton, C. D. Ichthyoplankton distribution and dispersal in the Tsitsikamma National Park marine reserve, South Africa. South African J. Mar. Sci. 17, 1–14 (1996).
Quinn, G. P. Ecology of the intertidal pulmonate limpet Siphonaria diemenensis Quoy et Gaimard. II. Reproductive patterns and energetics. J. Exp. Mar. Bio. Ecol. 117, 137–156 (1988).
Cirano, M. & Middleton, J. F. Aspects of the mean wintertime circulation along Australia’s southern shelves: numerical studies. J. Phys. Oceanogr. 34, 668–684 (2004).
Petrusevics, P. & Bye, J. A. T. The Leeuwin Current, wind, mean sea level and variability in Australian salmon larvae recruitment. In Proc Ocean & Atmosphere Pacific Int Conference 288–292 (1995).
Shanks, A. L. Pelagic larval duration and dispersal distance revisited. Biol. Bull. 216, 373–385 (2009).
Weersing, K. & Toonen, R. J. Population genetics, larval dispersal and connectivity in marine systems. Mar. Ecol. Prog. Ser. 393, 1–12 (2009).
Black, K. P. The relative importance of local retention and inter-reef dispersal of neutrally buoyant material on coral reefs. Coral Reefs 12, 43–53 (1993).
Porch, C. E. A numerical study of larval fish retention along the southeast Florida coast. Ecol. Modell. 109, 35–59 (1988).
Cowen, R. K., Lwiza, K. M., Sponaugle, S., Paris, C. B. & Olson, D. B. Connectivity of marine populations: open or closed? Science 287, 857–859 (2000).
Kaplan, D. M., Botsford, L. W., O’Farrell, M. R., Gaines, S. D. & Jorgensen, S. Model-based assessment of persistence in proposed marine protected area designs. Ecol. Appl. 19, 433–448 (2009).
Berumen, M. L. et al. Persistence of self-recruitment and patterns of larval connectivity in a marine protected area network. Ecol. Evol. 2, 444–52 (2012).
We are grateful to two anonymous reviewers for their valuable comments on an earlier version of the manuscript. This study was funded by the Australian Research Council (DP110101275 to L.B.B, J.W. and Luciana Möller, FT130101068 to L.B.B. and DE130101336 to E.v.S.). The present article is publication no. 58 of the Molecular Ecology Group for Marine Research (MEGMAR).
The authors declare no competing financial interests.
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Teske, P., Sandoval-Castillo, J., van Sebille, E. et al. Oceanography promotes self-recruitment in a planktonic larval disperser. Sci Rep 6, 34205 (2016). https://doi.org/10.1038/srep34205
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