Table 2 Optimized ESI-MS and ESI-MS/MS parameters as obtained during method optimization.

From: Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells

Analyte RT [min] Precursor ion [m/z] Ion species Product ions a [m/z] Relative intensity b CE a,c [eV] S-lens
DON 9.4 355.1 [M + Ac] 265.2/247.2 29% −17/−19 75
DON-3-sulfate 8.5 375.0 [M−H] 345.0/247.0 59% −21/−24 100
DON-15-sulfate 8.2 375.0 [M−H] 97.0/163.1 22% −35/−40 100
DON-3-glucuronide 8.8 471.1 [M−H] 265.0/175.0/441.0 93%/37% −27/−30/−23 150
DON-15-glucuronide 9.0 471.1 [M−H] 265.0/175.0/441.0 27%/3% −27/−30/−23 150
Deepoxy-DON 12.6 339.1 [M + Ac] 249.0/59.0 106% −15/−23 62
  1. aValues are given in the order quantifier ion/qualifier ion/qualifier ion 2 (in case of glucuronides).
  2. bSignal intensity of the qualifier transition in relation to the quantifier (qualifier/quantifier × 100).
  3. cCollision energy.