Figure 1 : Determination of GPCR transcript levels in human spermatozoa.

From: Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18

Figure 1

(A) Shown are the 20 most highly expressed GPCR transcripts in human spermatozoa from four different donors. For sperm donors 3 and 4, four different datasets of four independent semen samples each were generated (Sperm 1, 2, 3-1, 3-2, 3-3, 3-4, 4-1, 4-2, 4-3, 4-4) Data were compared to ten different testis samples (Testis 1–10), brain (B), colon (C), liver (LI), lung (LU) and skeletal muscle (SM). GPCRs are sorted by the mean FPKM in spermatozoa. The FPKM values are an indicator for expression strength of transcripts and are represented by color intensity. Ligand: describes which ligand is known for the respective GPCR. Associated function to sperm: describes whether a function of the respective GPCR is known in sperm. n.a.: not available. (B) RT-PCR analysis of GPCRs obtained from RNA-Seq. Gel electrophoresis of the amplified PCR products from cDNA samples of human sperm and testis samples. For GPR18 we detected both transcript variants. The highly expressed sperm-specific transcript CRISP2 was used as a positive control59. Used primer pairs were intron-spanning excluding gDNA amplified products. M: Marker in bp.