Figure 6 : Microglial depletion in the medial pre-frontal cortex by CSF1R inhibition.

From: Elimination of microglia improves cognitive function following cranial irradiation

Figure 6

(A,B) Immunofluorescence staining and laser scanning confocal microscopy for the IBA-1+ and CD68+ cells show that cranial irradiation lead to significant elevation in microglial number (40% and 25% respectively) in the pre-limbic (PrL) and infra-limbic (IL) cortices of the medial pre-frontal cortex (mPFC). (a1,b1) Representative high-resolution (60×) z stacks showed characteristic activated microglial morphology (IBA-1, a1 and CD68, b1) in the irradiated PrL compared to 0 Gy group. (C,D) Treatment with PLX5622 for 6 weeks eliminates 80–90% of IBA-1+ and CD68+ microglia from the control (0 Gy + PLX5622) and irradiated (9 Gy + PLX5622) mPFC. Data are presented as mean ± SEM (N = 4 mice/group). P values are derived from ANOVA and Bonferroni’s multiple comparisons test. *P < 0.05; **P < 0.001 compared with 0 Gy group and +P < 0.05; ++P < 0.01compared with 9 Gy group. Scale bars: 200 μm (A), 100 μm (B) and 50 μm (a1,b1).