Figure 5 : CSF1R inhibition reduces CD68+ activated microglia from the irradiated hippocampus.

From: Elimination of microglia improves cognitive function following cranial irradiation

Figure 5

(A) Immunofluorescence staining show that PLX5622 treatment for 6 weeks reduces ~70% of CD68+ activated microglia from the control (0 Gy + PLX5622) and irradiated (9 Gy + PLX5622) brains. (a1,a2) Representative high-resolution (60×) z stacks show ramified microglial morphology in the irradiated hippocampal dentate hilus (DH) and granule cell layer (GCL) compared to 0 Gy mice that received control chow. (B) Quantification (Autoquant and Imaris) of CD68+ activated microglia indicated an 80–90% reduction in the control and irradiated brains receiving PLX5622 (0 Gy + PLX5622 and 9 Gy + PLX5622) at 2 week and 6 week time points. (C) Analysis of pro-inflammatory markers from whole brains derived from irradiated mice (0 and 9 Gy) treated with PLX5622 for 1 week at 4 week post-irradiation show radiation-induced elevation in gene expression that was reduced significantly by PLX5622 treatment. Data are presented as mean ± SEM (N = 4 mice/group). P values are derived from ANOVA and Bonferroni’s multiple comparisons test. *P < 0.01; **P < 0.001 compared with 0 Gy group and +P < 0.01; ++P < 0.01compared with 9 Gy group. Scale bars: 200 μm (A) and 20 μm (a1,a2).