Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

11,000 years of craniofacial and mandibular variation in Lower Nubia

Abstract

The transition to agriculture was a key event in human history. The extent to which this transition is associated with biological changes in different world regions remains debated. Cultural and osteological records in Lower Nubia throughout the Holocene have been interpreted as a result of in situ differentiation or alternatively as migratory events and possible admixture with surrounding populations. Here we investigated the patterns of craniofacial and mandibular variation from Mesolithic hunting-gathering to late farming, a period spanning 11,000 years. We analyzed 102 adult specimens spanning five cultural horizons: Mesolithic, A-group, C-group, Pharaonic and Meroitic, by means of 3D geometric morphometric methods, in order to assess shape variation and diachronic patterns at the transition to farming and in subsequent periods. Our results highlight a strong morphometric distinction between Mesolithic hunter-gatherers and farmers as well as differences between transitional and intensive farmers in mandibular variation which is consistent with differential impact of selective pressures on different regions of the skull. This study corroborates a major biological change during the transition from hunting to farming, supporting the masticatory-functional hypothesis for the mandible and suggesting population continuity among farming populations throughout the Holocene based on the overall shape of the cranium.

Introduction

The history of prehistoric human populations from Lower Nubia during the Holocene has drawn great interest in anthropology for the richness of archaeological findings and the opportunity to examine the process of subsistence change from hunting and gathering to farming through time. The shift to agriculture was a key event in human history driving major biological and cultural change globally1,2. However research indicates that the nature and timing of this transition varied across world regions1,2,3,4. In Lower Nubia, biological affinities, in situ microevolutionary processes, population movements and the relationships among people from the Mesolithic to the Meroitic period remains highly debated5,6,7. Here we applied 3D geometric morphometric methods to investigate both cranial and mandibular variation among Mesolithic hunter-gatherers and early and late farmers.

Lower Nubia (or northern Nubia8) covers the area extending from southern (or Upper) Egypt to northern Sudan along the Nile valley, from the first to the second cataract9 (Fig. 1). Different cultural horizons, analysed in the present study, have been defined based on archaeological evidence of varying subsistence strategies: Mesolithic, A-group, C-group, Pharaonic and Meroitic.

Figure 1
figure 1

Map showing the area of the samples analysed (map created using Inkscape 0.91, www.inkscape.org).

Mesolithic Nubians (11,000–8,000 BCE) are associated with a hunting and gathering adaptation, particularly focussed on the exploitation of large game hunting plus some fishing and seed collecting5,10. They were characterized by a low density and dispersed population11.

A-group Nubians (3,300–2,800 BCE) represent a transition from Mesolithic to Neolithic9. They were semi-nomadic herders and rudimentary agriculturalists with evidence of domesticated animals and grains plus extensive fishing, hunting and gathering8,9,10,12. A-group Nubians could be descendants of Early Mesolithic/Late Neolithic Nubians or related to populations from Upper Nubia13. Archaeological evidence suggests that A-group Nubians represent an indigenous cultural development9,13. The main issue regarding this cultural horizon is the presence of a major hiatus around 5,000 to 8,000 years between Mesolithic and A-group as evidenced by a lack of well-preserved skeletal remains in the archaeological record between the Mesolithic and A-group horizon, making estimation of their ancestry difficult7,9,13.

C-group Nubians (2,300–1,800 BCE) share numerous cultural features with A-group such as presenting a mixed economy10,13,14. They also present an intensified agricultural regime and a much more complex culture9,15. Many studies suggest that C-group is a continuation of the A-group9,10,13,14 although there is a gap of a few centuries between the A- and C-group cultural horizons. The lack of archaeological evidence during this gap could be the result of political pressure from the Egyptian Old Kingdom pushing populations from Lower to Upper Nubia as well as of ecological factors associated with the desiccation of the Nile River leading to a reduction of the available resources8,9. Another hypothesis proposes that C-group Nubians are not only descendants from A-group but encompasses new people from the surrounding area7,8.

Pharaonic Nubians refer to populations living during the expansion of the Egyptian New Kingdom (1,550–1,070 BCE) from the eighteenth to the twentieth dynasty8,9. They are characterized by a very complex agriculture (use of irrigation systems) and animal domestication8. Their biological and ethnic affiliation remains debated with archaeological evidence showing a mix of Nubian and Egyptian cultures: they could be C-group assimilated to the Egyptian culture, “Egyptianized Nubians”9 or Egyptian migrants8,9,14.

Meroitic Nubians (100 BCE–350 CE) are associated with a fully farming and animal husbandry adaptation with a highly sophisticated culture displaying evidence of seasonal strategies, trade, and use of the waterwheel which increased the potential of food production and led to larger population sizes9,10,14,16. For almost a thousand years between the Pharaonic and Meroitic horizons, there is a lack of archaeological evidence known as the “Nubian Hiatus”, which is probably due to a drop of the Nile River making the area uninhabitable9,13. Debates persist whether Meroitic Nubians were descendants of C-group and/or Pharaonic Nubians or whether they represent a new ethnic group8.

Several models have been proposed to explain the biological variation among Nubian populations during the Holocene. The “population continuity” hypothesis suggested by Greene17, proposed population continuity from the Mesolithic through to the Christian period based on the analysis of craniofacial and dental data. Despite the fact that there is no physical continuity in occupation with some gaps in the archaeological record as mentioned above, this model emphasizes cultural and biological continuity with no evidence of a major population replacement over a period of 12,000 years9,17. This hypothesis has received support from different studies based on cranial measurements6,9,18,19, dental traits17,20,21,22 and archaeological artefacts13. Carlson and Van Gerven6 highlighted the importance of selective pressures in Lower Nubians proposing the “masticatory-functional hypothesis”, which suggests that a reduction in functional demands relating to mastication led to an alteration of facial morphology and a reduction of dental and mandibular dimensions in later farming populations. This hypothesis supposes that the shift to agriculture led to an increasing reliance on softer and processed foodstuffs, which is corroborated by independent evidence from dental microwear as well as from molecular studies of crop plant, and that consequently agriculturalists experience shorter and less intensive chewing2,5,6. However other studies found the morphological divergence among groups to be too great to be accounted for by continuity or adaptive change, suggesting the presence of a post-Pleistocene biological discontinuity due to population movements along the Nile corridor. This “population influx” hypothesis is mainly supported by dental variation studies7,23,24 but has also some support from cranial25 and postcranial analyses26.

Cranial morphology is shaped by both neutral evolutionary forces and adaptation to extrinsic factors27,28,29,30,31. While a consensus has emerged in recent years that global patterns of cranial shape variation can be explained to a large extent on the basis of a neutral model of population diversification27,28,29,31, previous research has emphasized that a response to modifications in masticatory behaviours could significantly influence overall skull morphology6,32,33,34,35,36,37 and the mandible in particular6,35,36. As such, craniometric data can be employed as a proxy for population biodistance, facilitating the investigation of both population history and adaptation to selective pressures in order to comprehend the biological processes involved during the transition from hunter-gathering to farming. Focusing on the Nile corridor is particularly interesting because of the richness of its archaeological and osteological evidence, the presence of cultural interactions and development of complex societies prior and during the time of the Egyptian Kingdom. Here, using 3D geometric morphometrics for the first time to quantify and visualize overall cranial size and shape variation across Nubian prehistory, our study aimed to evaluate both cranial and mandibular morphological patterns among Nubian series from 11,000 BCE to 350 CE (Table 1). Specifically, we tested the following hypotheses:

  1. i

    The population continuity hypothesis, according to which, we would expect to observe a relative homogeneous morphological pattern among all chrono-cultural groups with no clear distinction based on chronology and strong overlap in morphological variability among all groups.

  2. ii

    The masticatory-functional hypothesis, according to which, we would expect strong morphological differences localized in the mandible between dietary groups (hunter-gatherers, early farmers, intensive farmers) as well as a significant decrease in size and robustness of the face between hunter-gatherers and farmers.

  3. iii

    The population influx hypothesis, according to which, we would expect a significant differentiation between the Mesolithic specimens and the agricultural groups. We expect to see these morphological differences most strongly in overall skull morphology not only in the region related with masticatory processes but especially in the cranial morphology which is assumed to reflect population history more than dietary adaptation.

Table 1 Samples and related information.

Results

Overall cranial and mandibular shape variation underlines a strong distinction between the Mesolithic specimens and the other four cultural groups. Patterns of between-group differentiation among the four post-Mesolithic groups are more complex and differ between analyses based on either the cranium or the mandible.

Regarding size variation, Mesolithic Nubians show a larger average size for both the cranium and the mandible (Fig. 2). However significant differences in size between Mesolithic Nubians and all other cultural group were only found for the mandible (Supplementary Tables S3 and S4). No significant differences in size were detected among the early and late Nubian farmers for either the cranium or the mandible. Crania with a bigger centroid size corresponding to Mesolithic specimens have relatively wider zygomatic processes and smaller faces and vaults (Fig. 3). Bigger mandibles, also corresponding to Mesolithic specimens, had a wider and more robust corpus compared with smaller mandibles (Fig. 3). However allometric effects are very low for both the cranium and the mandible (Supplementary information; Tables S5 and S6).

Figure 2
figure 2

Boxplot of the centroid sizes by cultural horizon for the cranium (left) and the mandible (right).

Figure 3
figure 3

Impact of allometric effects observed on the crania and the mandibles.

Concerning shape variation, the distinction between Mesolithic specimens and the remaining groups is very clear on the first axis of both between-group principal components analyses performed on the cranium and on the mandible explaining 53% and 60% of the total variance, respectively (Fig. 4). Shape differences are pronounced between Mesolithic hunter-gatherers and post-Mesolithic early and late farmers, particularly in the mandible. Mesolithic Nubians have shorter, wider and more upright ramus and coronoid process, longer mandibular condyle and deeper, wider and upright corpus. They also exhibit lower and wider cranial vaults, shorter and wider faces, much wider zygomatics, more pronounced alveolar prognathism, more projected glabella, longer mastoid processes, lower and wider orbital apertures and a smaller nasal aperture. Mahalanobis distances between Mesolithic Nubians and any other cultural group were also larger for both the cranium and the mandible (Table 2; Fig. 5).

Figure 4
figure 4

Between-group PCA based on Procrustes residuals of landmark configurations and shape differences observed for the cranium and the mandible.

Table 2 Mahalanobis distances between cultural groups for the cranium (upper triangle) and the mandible (lower triangle).
Figure 5
figure 5

Neighbor-Joining trees based on Mahalanobis distances with bootstrap values for the cranium (left) and the mandible (right).

There is no such clear morphological differentiation between early and late farmer groups. Again shape differences are more pronounced for the mandible than for the cranium. Pharaonic and Meroitic Nubians tend to be distinguished from A-group and C-group specimens by exhibiting shorter, wider and less upright corpus. C-group specimens, contrary to the ones associated with Pharaonic and Meroitic cultural horizon, tend to have higher vaults, longer mastoid processes, smaller nasal apertures and slightly narrower faces. A-group specimens are in an intermediate position. Mahalanobis distances for both the cranium and the mandible indicate that A-group Nubians are slightly closer to C-group Nubians than any other cultural horizon (Table 2).

The cultural groups differ significantly in both cranial and mandibular shape variation (Table 3). The coefficient of determination was slightly higher for the cranium than for the mandible (R2 = 12.6% and 9.9%, respectively) when examining group affinity versus shape variation using MANOVA. The same analysis computed using the three dietary groups also demonstrates that diet has a significant impact on cranial and mandibular shape variation. The coefficient of determination was slightly higher when early farmers (A-group and C-group) and late farmers (Pharaonic and Meroitic) are considered as two groups rather than being grouped together (R2 = 8.7% compared with 6.1% for the cranium and R2 = 8% compared with 5.7% for the mandible). However, when the Mesolithic sample was excluded, the results for the MANOVA were significant only for the mandible with a smaller coefficient of determination (R2 = 5% for the cultural groups and R2 = 2.7% for the dietary groups).

Table 3 Results of MANOVA performed on the cranium and mandible from the chrono-cultural groups.

Discussion

Our results clearly depict a strong craniofacial and mandibular distinction in size and shape components between Mesolithic hunter-gatherers and early and late farmers. Mesolithic Nubians are characterized by a greater overall size and more robust mandibles, faces and zygomatics. These observations are congruent with previous studies highlighting a reduction of the facial robusticity from Mesolithic to A-group Nubians6,9,10,19,38. However, there is no significant diachronic pattern of reduction in cranial vault height and length6,9,10,19. Mesolithic Nubians tend to have lower vaults, greater alveolar prognathism, more projected glabella, and lower and wider orbits38. However, major shape changes described here concern the shape of the face and mandible, i.e., cranial and mandibular regions involved in the masticatory process, which is in agreement with morphological patterns found among hunter-gatherer populations from other regions33,35,36,39. Facial and mandibular robusticity suggests the presence of heavy chewing muscles and larger teeth, morphological characteristics expected for populations whose subsistence strategy is based on hunting-gathering6,34. We also confirm a reduction of both craniofacial and mandibular sizes which is consistent with other studies at a worldwide scale6,10,11,34,40.

While cranial and mandibular patterns in our analyses both indicate a strong distinction of Mesolithic Nubians and close morphological affinities between early and late farmers, the two anatomical datasets differ in terms of the pattern of differentiation found among cultural horizons. In evaluating our results alongside the three hypotheses outlined previously, we can draw the following conclusions. Neither the cranial nor the mandibular results align with the predictions of the “population continuity” hypothesis throughout the entire chronological sequence. The MANOVA results consistently found significant distinction between the Mesolithic and the other four cultural groups, and between early farmers and late farmers in the case of the mandible. However our results do not suggest any strong morphological differentiation occurring through time from the A-group to Meroitic cultural horizons, which is in agreement with previous studies that report no drastic change in skeletal series spanning from the A-group to the Christian period6,9,10,19. Nevertheless, the morphological distances between the A-group and C-group samples are relatively smaller, which is consistent with the hypothesis that C-group Nubians are direct descendants from A-group Nubians9,13,14. Similarly, shared morphological affinities between Pharaonic and Meroitic specimens support the notion that Meroitic Nubians were descendants from Pharaonic Nubians9,13,14. These two groups present shorter, wider and less upright corpus and less high vaults, morphological patterns also found in other farming populations36. However, the hypothesis that Pharaonic Nubians were descendants of the C-group Nubians that assimilated into Egyptian culture14 is not supported by our results which do not show any particular close affinities between these two groups. All these observations are more congruent with the general hypothesis of a regional continuity from A-group (non-intensive farmers) to Meroitic Nubians (intensive farmers) underlined by both biological and archaeological evidence6,7,9,10,13,19,32. Although we detect a significant strong distinction between Mesolithic and later Nubians, given the potential impact of selective pressures plus the temporal gap that could have been as much as 4,700 years, we cannot totally reject the presence of a relative biological continuity since the Mesolithic with no evidence of major population replacement. In case of a discontinuity with external arrivals of new group(s), we could expect morphological changes in overall skull morphology and not only concentrated in cranial regions related to mastication.

Our results do support the predictions of the masticatory-functional hypothesis as we see a significant difference among all three dietary groups (hunter-gatherers, early farmers, and late farmers) in mandibular variation. Also, the pattern of size and shape differences among these groups is consistent with the masticatory-functional hypothesis, given a trend towards decreasing size and robusticity throughout the transition from hunter-gathering to farming. Our results also show that mandibular morphology, which is presumed to be subject to selective (or non-neutral) pressures6,35,36,37, continues to be significantly impacted by cultural and economic variation from 3,200 BCE to 350 CE in parallel with the evolution of a mixed subsistence strategy to one fully dependent on farming9,13,14. The evidence for the cranium is more ambiguous, as MANOVA found significant differences between Mesolithic hunter-gatherers and farming groups, but no significant differences among the four farming groups. However, the main differentiation between hunter-gatherers and farmers lay in the relative width and robusticity in the facial skeleton, which is consistent with adaptive change due to masticatory-induced phenotypic plasticity6,41.

Finally the cranial results align with the predictions of the “population influx” hypothesis at the point of transition from hunter-gathering to farming, as suggested by the strong distinction between the Mesolithic sample and all four later cultural groups, but little differentiation among the four farming groups. Although cranial shape variation is mainly concentrated on the face, there is a slight difference observed in the vault height between Mesolithic and post-Mesolithic samples (Fig. 4). MANOVA results could support the “population influx” hypothesis between the Mesolithic Nubians and the beginning of the A-group as well as a late regional continuity in cranial shape among the four post-Mesolithic groups, as the pattern of cranial variation is assumed to reflect population history rather than adaptation due to diversifying natural selection28,29,31,36. This is also reinforced by the Neighbor-joining analysis and Mahalanobis distances, which shows a strong difference between the Mesolithic and all later groups (Fig. 5; Table 2), but does not strongly distinguish among the farming groups, despite considerable temporal variation among them. According to the “population influx” hypothesis, the large differentiation that we see in both cranial and mandibular shape between the Mesolithic and the A-group could be explained by an arrival of new people at the advent of the shift from hunting-gathering to farming, with additional adaptive changes in the masticatory apparatus (especially the mandible) as the reliance on farming intensified. This is consistent with previous cranial studies which have identified sharp cranial shape differentiation in the early Neolithic period of Europe with the influx of farmers from the Near East and Levant42,43,44. However, the differences between the cranial and mandibular results suggest that as the reliance on farming intensified throughout the later periods, the shape of the mandible continued to be affected in accordance with the masticatory-functional hypothesis, generating systematic differences in mandibular shape between the Mesolithic, early farmer, and late farmer groups.

Taken together, our results suggest a dramatic shift in cranial morphology between the Mesolithic and the A-group cultural group, with little perceptible change in cranial shape between A-group and the later farming groups. In the case of the mandible, we observe the largest morphological change between the Mesolithic and the A-group, but also see morphological differentiation between the early farmers (A- and C-group) and the later farming groups (Pharaonic and Meroitic specimens). While we test three hypothetical scenarios in this study, as had been suggested on the basis of previous research, it is worth noting that these three scenarios are not mutually exclusive. In situ masticatory adaptation with population continuity or alternatively with an influx of people is theoretically possible as well as a migration of farmers and biological continuity thereafter. Our data support the functional-masticatory hypothesis and demonstrate that the biomechanical changes associated with dietary change strongly affect the mandible and aspects of facial morphology but not have any clear discernible effect on the rest of the cranial structure. Yet further studies including larger comparative samples and the combination of morphometric analyses with ancient DNA are needed to precise biological continuity between Mesolithic and A-group or the influx of people from outside Lower Nubia.

In conclusion, our study corroborates major biological changes during the transition from hunting to farming in the lower Nubian region. It also demonstrates the differential impact of selective pressures in different skull regions and highlights that the mandible, in contrast to the cranium, reflects adaptation to subsistence strategy rather than patterns of variation related to neutral evolutionary forces such as gene flow and genetic drift. Our results give strong support to both the masticatory-functional hypothesis with morphological changes especially concerning the mandible and cranial regions involved in the mastication, and the “population continuity” hypothesis among post-Mesolithic groups based on the overall shape of the cranium.

Methods

Material

We analysed 69 crania and 97 mandibles of adult specimens of both sexes (Table 1). Data included in the present study were collected from skeletal remains associated with the Mesolithic (11,000–8,000 BCE), A-Group (3,300–2,800 BCE), C-Group (2,300–1,800 BCE), Pharaonic (1,800–1,200 BCE) and Meroitic (100 BCE–350 CE) cultural horizons from South Egypt/North Sudan located on the upper Nile between the first cataracts (Fig. 1). Mesolithic samples are from Wadi Halfa (Sudan) during the expedition to the Sudan led by the University of Colorado in 1964. A-group, C-group, Pharaonic and Meroitic samples are from Faras (Egypt) to Gamai (Sudan) during the Scandinavian Joint Expedition8. All specimens were anatomically considered adult with fully fused spheno-occipital synchodroses. Sub-adult specimens were excluded since their cranial morphology is not fully developed. Likewise adults displaying poor preservation or pathologies that could affect cranial shape were also excluded from the analyses. Crania and mandibles were collected from the same specimens and sex was equally balanced as much as possible. Sex was assessed with standard osteological techniques45.

Morphometric data

Each cranium and each mandible were digitized with a 3D surface scanner (Nextengine HD device; www.nextengine.com). One of the main advantages of 3D scanning is the possibility to get a virtual high-quality archive of osteological remains. Shape data were then collected in the form of three-dimensional coordinates of cranial landmarks. Thirty-nine and thirty-three homologous landmarks were respectively placed on each cranium and each mandible (Fig. 6) by a single observer (M.G.) using the Landmark Editor software46. Landmarks were chosen in order to reflect the overall skull shape and belong to all three types defined by Bookstein47. Anatomical description of all landmarks is presented in Tables S1 and S2.

Figure 6
figure 6

Facial and lateral views of a cranium and a mandible with 39 and 33 landmarks, respectively.

Repeatability was assessed through six non-consecutive extractions on ten specimens. The intra-observer error for each landmark (mean 0.8 mm) was below reported standard errors in craniometrics48. Missing bilateral landmarks were estimated by mirroring-imaging49. Missing landmarks in the sagittal plane or bilateral landmarks missing on both sides were estimated by the Thin-Plate-Spline interpolation50 which permits the mapping of missing points from available landmark configurations to the incomplete specimens in a way that the deformation between complete and incomplete specimens is as smooth (i.e. bending energy is minimized) as possible51. As recommended by Couette and White52, all specimens have less than 20% of missing data. The estimation of missing landmarks was performed using R53,54.

Geometric morphometrics and data processing

Landmark data was processed by 3D geometric morphometric methods. All craniofacial and mandibular landmark configurations were subjected to generalized Procrustes analysis (GPA55,56) which permits the extraction of geometric shape separate from overall (isometric) size. Landmark configurations were translated to a common centroid, scaled to unit centroid size, rotated by least squares fitting and then subjected to tangent space projection. This study focused on the symmetric component of shape variation, so all analyses are based on the averaged Procrustes coordinates of each landmark configuration and its mirror image in order to remove the effects of asymmetry57. All multivariate analyses were performed in R53. Differences in size between samples were studied by means of ANOVA tests and Tukey’s honestly significant differences (HSD). The pattern of shape variation and morphological differences were examined with between-group PCA which emphasizes among-group differences and is based on a between-groups variance-covariance matrix58. Among-group shape differences were visualized along the PCA axes. The PC scores from a normal total variance-covariance PCA required to account for 95% of the total variance were used to perform MANOVA as well as to generate the among-groups Mahalanobis distances. Neighbor-Joining trees (NJ59) were computed based on Mahalanobis distances using bootstrapping (1000 replications) to test the reliability of the clusters. MANOVA were then applied to test if there were significant morphological differences between samples from the five cultural horizons as well as from the three dietary groups. The allometric effect was tested using linear regressions using PC scores as dependent variables and the centroid size as independent variable.

Additional Information

How to cite this article: Galland, M. et al. 11,000 years of craniofacial and mandibular variation in Lower Nubia. Sci. Rep. 6, 31040; doi: 10.1038/srep31040 (2016).

References

  1. 1

    Bellwood, P. First Farmers: The Origins of Agricultural Societies. (Wiley-Blackwell, New York, 2004).

  2. 2

    Pinhasi, R. & Stock, J. The Bioarchaeology of the Transition to Agriculture (Wiley-Liss, New York, 2011).

  3. 3

    Thorpe, I. G. N. The origins of Agriculture in Europe (Routledge, London, 1996).

  4. 4

    Bar-Yosef, O. & Meadow, R. The origin of agriculture in the Near East. Last Hunters, First Farmers: New Perspectives on the Prehistoric Transition to Agriculture (eds Price, T. D. & Gebauer, A. B. ) 39–94 (School of American Research Press, Santa Fe, 1995).

  5. 5

    Green, D. L. & Armelagos, G. J. The Wadi-Halfa Mesolithic population. Department of Anthropology, University of Masachuetts, Amherst Research Report No. 11 (1972).

  6. 6

    Carlson, D. S. & Van Gerven, D. P. Masticatory function and post-Pleistocene evolution in Nubia. Am. J. Phys. Anthropol. 46, 495–506 (1977).

    CAS  Article  Google Scholar 

  7. 7

    Irish, J. D. Population Continuity vs. Discontinuity Revisited: Dental Affinities Among Late-Paleolithic Through Christian-Era Nubians. Am. J. Phys. Anthropol. 128, 520–535 (2005).

    Article  Google Scholar 

  8. 8

    Nielsen, O. V. Human remains: metrical and non-metrical anatomical variation. Odense, Denmark: Scandinavian Joint Expedition to Sudanese Nubia (1970).

  9. 9

    Carlson, D. S. & Van Gerven, D. P. Diffusion, biological determinism, and biocultural adaptation in the Nubian corridor. Am. Anthropol. 81, 561–580 (1979).

    Article  Google Scholar 

  10. 10

    Martin, D. L., Armelagos, G. J. & Van Gerven, D. P. The effects of socioecomomic change in prehistoric Africa: Sudanese Nubia as a case study in Paleopathology and the Origins of Agriculture (eds Cohen, M. N. & Armelagos, G. J. ) 193–214 (Academic Press, New York, 1984).

  11. 11

    Armelagos, G. J., Van Gerven, D. P., Goodman, A. H. & Calcagno, J. M. Post-Pleistocene Facial Reduction, Biomechanics and Selection Against Morphologically Complex Teeth: A Rejoinder to Macchiarelli and Bondioli. Hum. Evol. 4, 1–7 (1989).

    Article  Google Scholar 

  12. 12

    Sherif, N. M. Nubia before Napata in General history of Africa II: ancient civilizations of Africa (ed. Moktar, G. ) 245–277 (Berkeley: University of California Press, 1981).

  13. 13

    Adams, S. 1981. The importance of Nubia: a link between central Africa and the Mediterranean in General history of Africa II: ancient civilizations of Africa (ed. Moktar, G. ) 226–244 (Berkeley: University of California Press, 1981).

  14. 14

    Newman, J. L. The peopling of Africa: a geographic interpretation (New Haven: Yale University Press, 1995).

  15. 15

    Beckett, S. & Lovell, N. C. Dental Disease Evidence for agricultural intensification in the Nubian C-Group. Int. J. Osteoarchaeol. 4, 223–239 (1994).

    Article  Google Scholar 

  16. 16

    Hakem, A. A. The civilization of Napata and Meroe in General history of Africa II: ancient civilizations of Africa (ed. Mokhtar, G. ) 198–325 (Berkeley: University of California Press, 1981).

  17. 17

    Green, D. L. Dental Anthropology of Early Egypt and Nubia. J. Hum. Evol. 1, 315–324 (1972).

    Article  Google Scholar 

  18. 18

    Burnor, D. R. & Harris, J. E. Racial continuity in Lower Nubia: 12,000 to the Present. Proc. Indiana Acad. Sci. 77, 113–121 (1967).

    Google Scholar 

  19. 19

    Small, M. F. The Nubian Mesolithic: a consideration of the Wadi Halfa remains. J. Hum. Evol. 10, 159–162 (1981).

    Article  Google Scholar 

  20. 20

    Green, D. L., Ewing, G. H. & Armelagos, G. J. Dentition of a Mesolithic population from Wadi Halfa, Sudan. Am. J. Phys. Anthropol. 27, 41–56 (1967).

    Article  Google Scholar 

  21. 21

    Smith, P. & Shegev, M. The dentition of Nubians from Wadi Halfa, Sudan: an evolutionary perspective. J. Dent. Assoc. S. Afr. 43, 539–541 (1988).

    Google Scholar 

  22. 22

    Larsen, C. S. Bioarchaeology. (Cambridge: Cambridge University Press, 1997).

  23. 23

    Irish, J. D. & Turner, C. G. II . West African dental affinity of late Pleistocene Nubians: peopling of the Eurafrican-South Asian triangle II. Homo 41, 42–53 (1990).

    Google Scholar 

  24. 24

    Irish, J. D. Dental morphological affinities of Late Pleistocene through recent sub-Saharan and North African peoples. Bull. Mém. Soc. Anthropol. Paris 10, 237–272 (1998).

    Article  Google Scholar 

  25. 25

    Groves, C. P. & Thorne, A. The terminal Pleistocene and early Holocene populations of northern Africa. Homo 50, 249–262 (1999).

    Google Scholar 

  26. 26

    Holliday, T. W. Body size and proportions in the Late Pleistocene western Old World and the origins of modern humans (University of New Mexico, 1995).

  27. 27

    Relethford, J. H. & Harpending, H. C. Craniometric variation, genetic theory, and modern human origins. Am. J. Phys. Anthropol. 95, 249–270 (1994).

    CAS  Article  Google Scholar 

  28. 28

    Roseman, C. C. Detecting interregionally diversifying natural selection on modern human cranial form by using matched molecular and morphometric data. Proc. Natl. Acad. Sci. 101, 12824–12829 (2004).

    CAS  ADS  Article  Google Scholar 

  29. 29

    Harvati, K. & Weaver, T. D. Human Cranial Anatomy and the Differential Preservation of Population History and Climate Signatures. Anat Rec 288A, 1225–1233 (2006).

    Article  Google Scholar 

  30. 30

    Betti, L., Balloux, F., Hanihara, T. & Manica, A. The Relative Role of Drift and Selection in Shaping the Human Skull. Am. J. Phys. Anthropol. 141, 76–82 (2010).

    PubMed  Google Scholar 

  31. 31

    von Cramon-Taubadel, N. Evolutionary insights into global patterns of human cranial diversity: population history, climatic and dietary effects. J. Anthropol. Sci. 91, 1–36 (2014).

    Google Scholar 

  32. 32

    Carlson, D. S. Temporal variation in Prehistoric Nubian crania. Am. J. Phys. Anthropol. 45, 467–484 (1976).

    CAS  Article  Google Scholar 

  33. 33

    González-José, R. et al. Functional-cranial approach to the influence of economic strategy on skull morphology. Am. J. Phys. Anthropol. 128, 757–771 (2005).

    Article  Google Scholar 

  34. 34

    Sardi, M. L., Novellino, P. S. & Pucciarelli, H. M. Craniofacial morphology in the Argentine center-west: Consequences of the transition to food production. Am. J. Phys. Anthropol. 130, 333–343 (2006).

    Article  Google Scholar 

  35. 35

    Paschetta, C. et al. The influence of masticatory loading on craniofacial morphology: a test case across technological transitions in the Ohio valley. Am. J. Phys. Anthropol. 141, 297–314 (2010).

    Article  Google Scholar 

  36. 36

    von Cramon-Taubadel, N. Global human mandibular variation reflects differences in agricultural and hunter-gatherer subsistence strategies. Proc. Nat. Acad. Sci. 108, 19546–19551 (2011).

    CAS  ADS  Article  Google Scholar 

  37. 37

    Noback, M. L. & Harvati, K. The contribution of subsistence to global human cranial variation. J. Hum. Evol. 80, 34–50 (2015).

    Article  Google Scholar 

  38. 38

    Pudlo, A. Population of Nubia up to the 16th century BC. Anthropol. Rev. 62, 57–66 (1999).

    Google Scholar 

  39. 39

    Pinhasi, R. & von Cramon-Taubadel, N. A Craniometric Perspective on the Transition to Agriculture in Europe. Hum. Biol. 84, 45–66 (2012).

    Article  Google Scholar 

  40. 40

    Pinhasi, R., Eshed, V. & Shaw, P. Evolutionary Changes in the Masticatory Complex Following the Transition to Farming in the Southern Levant. Am. J. Phys. Anthropol. 135, 136–148 (2008).

    CAS  Article  Google Scholar 

  41. 41

    Calgano, J. M. Dental reduction in Post-Pleistocene Nubia. Am. J. Phys. Anthropol. 70, 349–363 (1986).

    Article  Google Scholar 

  42. 42

    Pinhasi, R. & von Cramon-Taubadel, N. Craniometric data supports demic diffusion model for the spread of agriculture into Europe. PLoS One 4, e6747 (2009).

    ADS  Article  Google Scholar 

  43. 43

    von Cramon-Taubadel, N. & Pinhasi, R. Craniometric data support a mosaic model of demic and cultural Neolithic diffusion to outlying regions of Europe. Proc. R. Soc. B. 278, 2874–2880 (2011).

    Article  Google Scholar 

  44. 44

    von Cramon-Taubadel, N., Stock J. & Pinhasi, R. Skull and limb morphology differentially track population history and environmental factors in the transition to agriculture in Europe. Proc. R. Soc. B. 280, 20131337 (2013).

    Article  Google Scholar 

  45. 45

    White, T. D. & Folkens, P. A. The Human Bone Manual (Elsevier, Amsterdam, 2005).

  46. 46

    Wiley, D. F. Landmark v 3.0. Institute for Data Analysis and Visualization, University of California, Davis (2005).

  47. 47

    Bookstein, F. L. Morphometric Tools for Landmark Data: Geometry and Biology. (Cambridge University Press, New York, 1991).

  48. 48

    Bräuer, G. Osteometrie in: Anthropologie: Handbuch der vergleichenden Biologie des Menschen, Band 1 (eds Knusmann, R. & Martin, R. ) 160–231 (Stuttgart, 1988).

  49. 49

    Mardia, K. V. & Bookstein, F. L. Statistical assessment of bilateral symmetry of shapes. Biometrika 87, 285–300 (2000).

    MathSciNet  Article  Google Scholar 

  50. 50

    Gunz, P., Mitteroecker, P., Neubauer, S., Weber, G. H. & Bookstein, F. L. Principles for the virtual reconstruction of hominin crania. J. Hum. Evol. 57, 48–62 (2009).

    Article  Google Scholar 

  51. 51

    Mitteroecker, P. & Gunz, P. Advances in geometric morphometrics. Evol. Biol. 36, 235–247 (2009).

    Article  Google Scholar 

  52. 52

    Couette, S. & White, J. 3D geometric morphometrics and missing data. Can extant taxa give clues for the analysis of fossils primates? C.R. Palevol. 9, 423–433 (2010).

    Article  Google Scholar 

  53. 53

    R Development Core Team. R: a language and environment for statistical computing. Version 2.15.0. Vienna: R Foundation for Statistical Computing (2015).

  54. 54

    Schlager, S. Morpho: calculations and visualizations related to geometric morphometrics. R package version 0.21. Available at: http://sourceforge.net/project/morpho-rpackage/ (2012).

  55. 55

    Gower, J. C. Generalized Procrustes analysis. Psychometrika 40, 33–51 (1975).

    MathSciNet  Article  Google Scholar 

  56. 56

    Rohlf, F. J. Statistical power comparisons among alternative morphometric methods. Am. J. Phys. Anthropol. 111, 463–478 (2000).

    CAS  Article  Google Scholar 

  57. 57

    Klingenberg, C. P., Barluenga, M. & Meyer, A. Shape analysis of symmetric structures: quantifying variation among individuals and asymmetry. Evol 56, 1909–1920 (2002).

    Article  Google Scholar 

  58. 58

    Mitteroecker, P. & Bookstein, F. Linear discrimination, ordination, and the visualization of selection gradients in modern morphometrics. Evol. Biol. 38, 100–114 (2011).

    Article  Google Scholar 

  59. 59

    Saitou, N. & Nei, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425 (1987).

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We are grateful to the following institutions: University of Colorado (Boulder) and Panum Institute (Copenhagen) and to Niels Lynnerup for facilitating access to the collections. We thank Julien Corny and two anonymous reviewers for their constructive comments on our manuscript. This research was supported by RP’s Irish Research Council Advanced Research Project Grant (RPG2013-2).

Author information

Affiliations

Authors

Contributions

M.G., N.V.C.-T. and R.P. designed the research. M.G. sampled and analysed the data. M.G., D.P.V.G., N.V.C.-T. and R.P. wrote the paper. All authors discussed the results and approved the final manuscript.

Corresponding author

Correspondence to Manon Galland.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Galland, M., Van Gerven, D., Von Cramon-Taubadel, N. et al. 11,000 years of craniofacial and mandibular variation in Lower Nubia. Sci Rep 6, 31040 (2016). https://doi.org/10.1038/srep31040

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1038/srep31040

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing