Figure 2 : Tbr2 is required for the production of both IPCs and oRGs in the developing ferret cortex.

From: An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals

Figure 2

(a) Dorsal views of the electroporated brain. Arrows indicate GFP-positive transfected areas. (b) IUE was performed at E30, E33 or E37, and coronal sections of the cerebral cortex were prepared 3 days later. The sections were stained with anti-Tbr2 antibody and Hoechst 33342. CP, cortical plate; IZ, intermediate zone; OSVZ, outer subventricular zone; ISVZ, inner subventricular zone; VZ ventricular zone. Scale bar = 100 μm. (c,d) Quantification of Tbr2-positive cells in GFP-positive transfected cells in (b). (em) pCAG-DN-Tbr2 and pCAG-GFP were introduced to the ferret cerebral cortex by using IUE at E33. Coronal sections of the cerebral cortex were prepared at E36 and stained with Hoechst 33342 plus either anti-Tbr2 antibody (eg), anti-Pax6 antibody (hj) or anti-Sox2 antibody (km). It should be noted that the anti-Tbr2 antibody used here recognizes endogenous Tbr2 but does not recognize transfected DN-Tbr2. (e) Tbr2 immunohistochemistry. The numbers of Tbr2-positive cells were reduced by DN-Tbr2 in the OSVZ and the ISVZ. (f,g) Quantification of GFP-positive cells co-localized with Tbr2 in the OSVZ (f) and in the ISVZ (g). The proportions of GFP-positive cells that co-localized with Tbr2 were significantly decreased by inhibiting Tbr2 function (n = 3 animals; *p < 0.05; Student’s t-test). (h) Pax6 immunohistochemistry. The numbers of Pax6-positive cells were reduced by DN-Tbr2 in the SVZ. (i,j) Quantification of GFP-positive cells co-localized with Pax6 in the OSVZ (i) and in the ISVZ (j). The proportions of GFP-positive cells that co-localized with Pax6 were significantly decreased by inhibiting Tbr2 function (n = 3 animals; *p < 0.05; Student’s t-test). (k) Sox2 immunohistochemistry. The numbers of Sox2-positive cells were reduced by DN-Tbr2 in the SVZ. (l,m) Quantification of GFP-positive cells co-localized with Sox2 in the OSVZ (l) and in the ISVZ (m). The proportions of GFP-positive cells that co-localized with Sox2 were significantly decreased by inhibiting Tbr2 function (n = 3 animals; *p < 0.05; Student’s t-test). Bars present mean ± SD. Scale bar = 50 μm.