Figure 1 : Regional difference of the abundance of SVZ progenitors in the ferret cerebral cortex during development.

From: An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals

Figure 1

Immunostaining using the cerebral cortex of ferret embryos. The cerebral cortex was prepared at the indicated time points, and coronal sections were subjected to immunostaining for Tbr2 (ad), Pax6 (eh) and Sox2 (i). The areas within the boxes in (a,e,i) were magnified and are shown in the lower panels (1′, 1″, 2′, 2″, 3′ and 3″). ((a), top) The schematic drawings representing coronal sections we analyzed. Immunohistochemical images within the boxes are shown below. d, dorsal; v, ventral; m, medial; l, lateral. ((a), bottom) Tbr2-positive IPCs were more abundant in certain cortical areas (2′, 3′) than others (2″, 3″). (b) A representative image at E36 containing four ROIs (b1–b4) used for quantification of Tbr2 signal intensities. (c) Tbr2 signal intensities within the ROI b2 and b4 were measured along the radial axis and plotted against the distance from the ventricular surface. Note that Tbr2 signal intensities in the OSVZ were different between ROI b2 and b4, while those in the ISVZ were almost the same. (d) Tbr2 signal intensities in the OSVZ of four ROIs (b1–b4). (e) Pax6-positive oRGs were more abundant in certain cortical areas (2′, 3′) than others (2″, 3″). (f) A representative image at E36 containing three ROIs (f1–f3) used for quantification of Pax6 signal intensities. (g) Pax6 signal intensities within the ROI f2 and f3 were measured along the radial axis and plotted against the distance from the ventricular surface. Note that Pax6 signal intensities in the SVZ were different between ROI f2 and f3, while those in the VZ were almost the same. (h) Pax6 signal intensities in the SVZ of three ROIs (f1–f3). (i) Sox2-positive oRGs were more abundant in certain cortical areas (2′, 3′) than others (2″, 3″). V, ventricular surface; P, pial surface. Scale bars = 500 μm ((a,e,i,) upper; (b,f)) and 100 μm ((a,e,i,) lower).