Cumulative egg release (n = 8 ± SE) under (A,E) high tide between 8:00 and 12:00 h; (B,F) high tide between 12:01 and 16:00 h; (C,G) high tide between 16:01 and 20:00 h; and (D,H) constant immersion (no tidal regime). Black and white bars represent the means in each tank. Dark grey bars represent the means in tank with no tidal regime. Different letters above bars indicate significant differences (PERMANOVA, p < 0.05). Light grey shading indicates the night periods and longitudinal lines (black dotted lines) separate the timing of high and low tide periods.