Figure 5 | Scientific Reports

Figure 5

From: Efficient production of multi-modified pigs for xenotransplantation by ‘combineering’, gene stacking and gene editing

Figure 5

Reduced cytokine-induced upregulation of E-selectin and MHC class-II and caspase 8 induction in multi-transgenic GGTA1 KO porcine aortic endothelial cells.

Porcine aortic endothelial cells from a wild-type animal and multi-transgenic GGTA1-deficient piglet 779 were cultured with medium or cytokines. (A) Upregulation of E-selectin after 2 days exposure to 50 ng/mL TNFα. (B) Upregulation of porcine MHC class-II after 3 days stimulation with 8 ng/mL or 50 ng/mL IFNγ (N = 3). p < 0.05, two-tailed unpaired t-test. Data are shown as mean ± standard deviation (SD). (C) Luminescence assay for induction of caspase 8 activity in kidney fibroblasts after 24 h 20 ng/mL TNFα 10 μg/mL cycloheximide.

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